Identification of autoantibody reactive integral brain membrane antigens

Narendran, A.; Hoffman, Steven A.

doi:10.1016/0022-1759(88)90178-0

Cited by 20 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…associations between ANA, dsDNA, splenomegaly, brain atrophy, and neurodegeneration) supports this notion (Sakic et al, 2000a;Ballok et al, 2003Ballok et al, , 2006. Binding of brain-reactive autoantibodies (BRAs) to CNS tissue also appears to have detrimental consequences Hoffman et al, 1978Hoffman et al, , 1988Narendran and Hoffman, 1988;Sidor et al, 2005). While the mechanism by which circulating BRAs access the brain is not well understood (Hoffman and Harbeck, 1989), aberrant behavioral and emotional manifestations in human and murine forms of lupus does suggest that multiple CNS antigens and sites are targeted (Hoffman and Madsen, 1990;Hoffman et al, 1987;Crimando and Hoffman, 1992;Sakic et al, 1994;Huerta et al, 2006;DeGiorgio et al, 2001;Kowal et al, 2004).…”

Section: Prelude To Cns Damage: Disruption Of the Blood-brain Barriermentioning

confidence: 89%

Neuroimmunopathology in a murine model of neuropsychiatric lupus

Ballok

2007

Brain Research Reviews

View full text Add to dashboard Cite

Animal models are extremely useful tools in defining pathogenesis and treatment of human disease. For many years researchers believed that structural damage to the brain of neuropsychiatric (NP) patients lead to abnormal mental function, but this possibility was not extensively explored until recently. Imaging studies of NP-systemic lupus erythematosus (SLE) support the notion that brain cell death accounts for the emergence of neurologic and psychiatric symptoms, and evidence suggests that it is an autoimmunity-induced brain disorder characterized by profound metabolic alterations and progressive neuronal loss. While there are a number of murine models of SLE, this article reviews recent literature on the immunological connections to neurodegeneration and behavioral dysfunction in the Fas-deficient MRL model of NP-SLE. Probable links between spontaneous peripheral immune activation, the subsequent central autoimmune/inflammatory responses in MRL/MpJ-Tnfrsf6 lpr (MRL-lpr) mice and the sequential mode of events leading to Fas-independent neurodegenerative autoimmune-induced encephalitis will be reviewed. The role of hormones, alternative mechanisms of cell death, the impact of central dopaminergic degeneration on behavior, and germinal layer lesions on developmental/regenerative capacity of MRL-lpr brains will also be explored. This model can provide direction for future therapeutic interventions in patients with this complex neuroimmunological syndrome.

show abstract

Section: Prelude To Cns Damage: Disruption Of the Blood-brain Barriermentioning

confidence: 89%

Neuroimmunopathology in a murine model of neuropsychiatric lupus

Ballok

2007

Brain Research Reviews

View full text Add to dashboard Cite

show abstract

“…The technique to extract integral membrane proteins from the brain has been previously described [24]. A two month C3H/HeJ brain was used in the BRAA ELISA.…”

Section: Methodsmentioning

confidence: 99%

Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays

2014

View full text Add to dashboard Cite

BackgroundAn accurate method that can diagnose and predict lupus and its neuropsychiatric manifestations is essential since currently there are no reliable methods. Autoantibodies to a varied panel of antigens in the body are characteristic of lupus. In this study we investigated whether serum autoantibody binding patterns on random-sequence peptide microarrays (immunosignaturing) can be used for diagnosing and predicting the onset of lupus and its central nervous system (CNS) manifestations. We also tested the techniques for identifying potentially pathogenic autoantibodies in CNS-Lupus. We used the well-characterized MRL/lpr lupus animal model in two studies as a first step to develop and evaluate future studies in humans.ResultsIn study one we identified possible diagnostic peptides for both lupus and altered behavior in the forced swim test. When comparing the results of study one to that of study two (carried out in a similar manner), we further identified potential peptides that may be diagnostic and predictive of both lupus and altered behavior in the forced swim test. We also characterized five potentially pathogenic brain-reactive autoantibodies, as well as suggested possible brain targets.ConclusionsThese results indicate that immunosignaturing could predict and diagnose lupus and its CNS manifestations. It can also be used to characterize pathogenic autoantibodies, which may help to better understand the underlying mechanisms of CNS-Lupus.

show abstract

“…The protocol used for the extraction of the integral membrane proteins used in the ELISA and Western blotting techniques was previously described by (Narendran and Hoffman, 1988). Briefly, the integral membrane proteins were suspended in phosphate buffer saline (PBS), if being used for ELISA, or 1.0M Tris (pH 6.8) if being used for Western blotting.…”

Section: Methodsmentioning

confidence: 99%

Circulating brain-reactive autoantibodies and behavioral deficits in the MRL model of CNS lupus

Williams

Šakić

Hoffman

2010

Journal of Neuroimmunology

Self Cite

View full text Add to dashboard Cite

Brain reactive autoantibodies (BRAA) are hypothesized to play a role in the neuropsychiatric manifestations that accompany systemic lupus erythematosus (SLE). The present study tests the proposed relation between circulating BRAA and behavioral deficits in lupus-prone MRL/lpr mice. Two age-matched cohorts born at different times were used to test the relationship in the context of altered disease severity. Significant correlations between autoimmunity and behavior were detected in both cohorts. These results are the first to report correlations between behavior and autoantibodies to integral membrane proteins of brain, supporting the hypothesis that BRAA contribute to the behavioral dysfunction seen in lupus.

show abstract

Identification of autoantibody reactive integral brain membrane antigens

Cited by 20 publications

References 27 publications

Neuroimmunopathology in a murine model of neuropsychiatric lupus

Neuroimmunopathology in a murine model of neuropsychiatric lupus

Diagnosis and early detection of CNS-SLE in MRL/lpr mice using peptide microarrays

Circulating brain-reactive autoantibodies and behavioral deficits in the MRL model of CNS lupus

Contact Info

Product

Resources

About