Identification of Auxin Metabolites in Brassicaceae by Ultra-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Revelou, Panagiota-Kyriaki; Kokotou, Maroula G.; Constantinou-Kokotou, Violetta

doi:10.3390/molecules24142615

Cited by 13 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 4-chloroindole-3-acetic acid, an analog of IAA was detected from Microbacterium testaceum Y411 extract at m/z of 209.16 by HRMS analysis. A similar result was reported by Revelou et al. (2019) for plant IAA which found 4-chloroindole-3-acetic acid at m/z ratios 208.0167 and 210.0136.…”

Section: Discussionsupporting

confidence: 88%

“…The FTIR spectrum of the bacterial extract revealed that the detected functional groups present in auxin. Further validation through HRMS results in terms of m/z of IAA from the extracted sample was matched with the commercially available standard compound and found the IAA metabolites ( Revelou et al., 2019 ). The 4-chloroindole-3-acetic acid, an analog of IAA was detected from Microbacterium testaceum Y411 extract at m/z of 209.16 by HRMS analysis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

View full text Add to dashboard Cite

Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…They demonstrated that the colored product was an oxidation of IAA in the presence of Fe II, forming the N-hydroxy-3-indole-acetic acid product, which could be formed from any other indole analog compound and yield other indole derivated. Revelou et al (2019) demonstrated that indole-3-acetic acid (IAA) and its metabolites in plants of the Brassicaceae family belong to IAA amide conjugates with amino acids bearing a free carboxylic group or a methyl ester group. This was achieved through high-resolution mass spectrometry (HRMS) and ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) methodology.…”

Section: Discussionmentioning

confidence: 99%

Comparative study between Salkowski reagent and chromatographic method for auxins quantification from bacterial production

Guardado-Fierros,

Tuesta-Popolizio,

Lorenzo-Santiago

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

IntroductionThe Salkowski reagent method is a colorimetric technique used to determine auxin production, specifically as indole-3-acetic acid (IAA). It was developed to determine indoles rapidly; however, it does not follow Beer's law at high concentrations of IAA. Thus, there could be an overestimation of IAA with the Salkowski technique due to the detection of other indole compounds.MethodsThis study aims to compare the Salkowski colorimetric method versus a chromatographic method to evidence the imprecision or overestimation obtained when auxins, such as indole-acetic acid (IAA), are determined as traits from promoting growth plant bacteria (PGPB), using ten different strains from three different isolation sources. The analysis used the same bacterial culture to compare the Salkowski colorimetric and chromatographic results. Each bacterium was cultivated in the modified TSA without or with tryptophan for 96 h. The same supernatant culture was used in both methods: Salkowski reagent and ultra-performance liquid chromatography coupled with a Mass Spectrometer (LC-MS/MS).ResultsThe first method indicated 5.4 to 27.4 mg L-1 without tryptophan in ten evaluated strains. When tryptophan was used as an inductor of auxin production, an increase was observed with an interval from 4.4 to 160 mg L-1. The principal auxin produced by all strains was IAA from that evaluated by the LC-MS/MS method, with significantly higher concentration with tryptophan addition than without. Strains belonging to the Kocuria genus were highlighted by high IAA production. The indole-3-propionic acid (IPA) was detected in all the bacterial cultures without tryptophan and only in K. turfanensis As05 with tryptophan, while it was not detected in other strains. In addition, indole-3-butyric acid (IBA) was detected at trace levels (13-16 µg L-1).ConclusionsThe Salkowski reagent overestimates the IAA concentration with an interval of 41-1042 folds without tryptophan and 7-16330 folds with tryptophan as inductor. In future works, it will be necessary to determine IAA or other auxins using more suitable sensitive techniques and methodologies.

show abstract

“…AN090126 has been demonstrated to exhibit antimicrobial activity against bacterial and fungal pathogens. In this context regarding biological activities of PGP compounds, this study highlighted the production of different kinds of interesting bioactive molecules such as germicidins (antibiotics, spore germination regulators) [ 56 ]; siderophores such as desferrioxamine E (iron uptake and transport) [ 57 ] and nocardamin (prevents plant defense against infections) [ 58 ]; many antibiotics such as CDA, prodiginines, and actinorhodin [ 59 ]; and tryptophan metabolites are related to IAA (PGP compound) [ 60 ], while kynurenic acid play a role on ethylene/auxin balance [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers

et al. 2023

View full text Add to dashboard Cite

The use of chemical fertilizers and pesticides has caused harmful impacts on the environment with the increase in economic burden. Biofertilizers are biological products containing living microorganisms capable of improving plant growth through eco-friendly mechanisms. In this work, three actinobacterial strains Streptomyces violaceoruber, Streptomyces coelicolor, and Kocuria rhizophila were characterized for multiple plant growth promoting (PGP) traits such as indole acetic acid production, phosphate solubilization, N2-fixation, and drought and salt tolerance. Then, these strains were investigated for their secreted and cellular metabolome, revealing a rich arsenal of bioactive molecules, including antibiotics and siderophores, with S. violaceoruber being the most prolific strain. Furthermore, the in vivo assays, performed on tomato (Solanum lycopersicum L.), resulted in an improved germination index and the growth of seedlings from seeds treated with PGP actinobacteria, with a particular focus on S. violaceoruber cultures. In particular, this last strain, producing volatile organic compounds having antimicrobial activity, was able to modulate volatilome and exert control on the global DNA methylation of tomato seedlings. Thus, these results, confirming the efficacy of the selected actinobacteria strains in promoting plant growth and development by producing volatile and non-volatile bioactive molecules, can promote eco-friendly alternatives in sustainable agriculture.

show abstract

Identification of Auxin Metabolites in Brassicaceae by Ultra-Performance Liquid Chromatography Coupled with High-Resolution Mass Spectrometry

Cited by 13 publications

References 42 publications

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

Comparative study between Salkowski reagent and chromatographic method for auxins quantification from bacterial production

Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers

Contact Info

Product

Resources

About