Identification of bacillary microbial contaminants and food poisoning agents from ukrainian plant raw materials and products

Pylypenko, Inna; Pylypenko, L.; Yamborko, Anna; Ilyeva, Olena; Kotlyar, Evgeniy; Babenko, Dmytro

doi:10.24263/2304-974x-2017-6-1-3

Cited by 3 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the 53 strains isolated from the cooled dough, 24 strains were B. subtilis, 17 were B. cereus, 10 were B. pumilis, 2 were B. licheniformis. Our studies also show an abundance of bacillary contamination of plant [39,40] and animal products [41,42].…”

Section: Molecular Genetic Methods Of Microbiological Control Of Regumentioning

confidence: 68%

“…Methods for rapid determination of regulated microbiological parameters that identify the presence of heat-resistant food pathogens diseases are based on the determination of molecular genetic features [24, 31-32, 37-38, 44-52]. The analytical studies are performed on the basis of modern literary sources and some of own results [27,34,35,[39][40][41][42].…”

Section: Methodsmentioning

confidence: 99%

“…However, we selected only the nhe A gene for PCR, given its greatest prevalence and detectable visual toxicity, which is associated with a major role in food poisoning [39,41,42]. The PCR with specific primers nhe AF and nhe AR, matched to the site of the nhe A gene, confirmed the belonging of all tested collection strains of B. cereus to the enterotoxigenic species of B. cereus, whereas in PCR analysis of the DNA of the collection species G. stearothermophilus and Paenibacillus polymyxa and in negative control (PCR mixture without DNA), no amplification products were detected.…”

Section: Molecular Genetic Methods Of Microbiological Control Of Regumentioning

confidence: 99%

See 2 more Smart Citations

Methodology for accelerated monitoring and assurance of sanitary quality and food safety

Pylypenko¹,

Pylypenko²,

Yamborko³

et al. 2017

Ukr. food j.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Molecular Genetic Methods Of Microbiological Control Of Regumentioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Section: Molecular Genetic Methods Of Microbiological Control Of Regumentioning

confidence: 99%

See 1 more Smart Citation

Methodology for accelerated monitoring and assurance of sanitary quality and food safety

Pylypenko¹,

Pylypenko²,

Yamborko³

et al. 2017

Ukr. food j.

Self Cite

View full text Add to dashboard Cite

show abstract

Prevalence, Toxin Genes, and Antibiotic Resistance Profiles of Bacillus cereus Isolates from Spices in Antalya and Isparta Provinces in Türkiye

Çürek,

Geniş,

Özden Tuncer

et al. 2023

Indian J Microbiol

View full text Add to dashboard Cite

Identification of Food Pathogens and Determination of Their Distribution Level in Ukrainian Food Products of Animal and Plant Origin by PCR Method

Berhilevych¹,

Pylypenko²,

Касянчук³

et al. 2019

ХНТ

View full text Add to dashboard Cite

The foodborne pathogens cause serious public health problems in each country. In this regard, microbiological investigation is included in food safety management of the food chain. Molecular methods and mostly polymerase chain reaction (PCR) are considered highly sensitive, specific and rapid methods for pathogens detection from raw material and food. This study describes the using of specially designed and highly specific primers for PCR to identify 5 common and especially dangerous causeve agents of food poisoning and disease and to determine their level of distribution in food of animal and plant origin. The studies included the identification of methicillin-resistant Staphylococcus aureus (MRSA) and Cronobacter spp. (E. sakazakii) from raw milk, Shiga toxin-producing strains of Escherichia coli (STEC) from beef and swine carcasses, Bacillus cereus and Clostridium perfringens from various types of plant and animal raw materials and products of its processing - fruits, vegetables, berries, dried and preserved products, food concentrates, half-canned food. A total of 397 food samples were investigated to detect these pathogens using classical bacteriological methods and PCR. It was found that the distribution of foodborne pathogens in the studied products of animal and plant origin was as follows: Staphylococcus aureus (MRSA) and Cronobacterspp. (E. sakazakii) in raw cow milk in 6.5% and 19.4% of cases, respectively; shiga-toxin-producing Escherichia coli (STEC) from beef and pork carcasses in 8.1% and 5.7%; Bacillus cereus and Clostridium perfringens from different types of plant and animal raw materials and their processing products averages 27.5 % and 7.7 %, respectively. The advantages of molecular biological methods to which the PCR method relates, include their speed, as well as the specificity of identification of microorganisms by the features of genetic regions of genes that carry information about their pathogenicity factors. It has been found that the rate of detection of these pathogens when using the PCR method in comparison with classical methods increases at least 5-9 times. This data will be useful for assessing microbiological risk and will help authorities develop strategies to reduce consumer health risks.

show abstract

Identification of bacillary microbial contaminants and food poisoning agents from ukrainian plant raw materials and products

Abstract: Keywords:Bacilli, Food Safety B. cereus PCR Phenotypic

Cited by 3 publications

References 5 publications

Methodology for accelerated monitoring and assurance of sanitary quality and food safety

Methodology for accelerated monitoring and assurance of sanitary quality and food safety

Prevalence, Toxin Genes, and Antibiotic Resistance Profiles of Bacillus cereus Isolates from Spices in Antalya and Isparta Provinces in Türkiye

Identification of Food Pathogens and Determination of Their Distribution Level in Ukrainian Food Products of Animal and Plant Origin by PCR Method

Contact Info

Product

Resources

About