Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.)

Perlińska-Lenart, Urszula; Piłsyk, Sebastian; Gryz, Elżbieta; Turło, Jadwiga; Hilszczańska, Dorota; Kruszewska, Joanna S.

doi:10.1007/s00203-020-02002-x

Cited by 30 publications

(19 citation statements)

References 71 publications

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“…For example, Bacillus, belonging to the phylum Firmicutes, was the dominant bacterial genus detected in the T. indicum ascocarp in the present study (28.25%). It was also observed in the ascocarps of other species, such as T. melanosporum (Antony-Babu et al 2014), T. panzhihuaensis (Wan and Liu 2014) and T. aestivum (Urszula et al 2020). Bacillus simplex has been reported to be a member of the plant growth-promoting rhizobacteria (Mesanza et al 2019;Hansen et al 2020).…”

Section: Discussionmentioning

confidence: 94%

“…Rhizobium and Bradyrhizobium, members of the class Alphaproteobacteria, were the other two important genera detected in the T. indicum ascocarps, the presence and predominance of which have been reported in several other tru e species, such as T. borchii (Barbieri et Compared to bacteria, the diversity and abundance of fungi was much lower in the T. indicum ascocarps. Urszula et al (2020) proposed that Tuber FBs selectively recruit microorganisms to their gleba, but the mechanisms of this recruitment remain unknown. As expected, Tuber was present in all the ascocarps and soils inside the samples.…”

Section: Discussionmentioning

confidence: 99%

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Microbial Communities of Ascocarps and Soils in a Natural Habitat of Tuber Indicum

Wang

Guo

et al. 2021

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Truffles are the fruiting bodies of hypogeous fungi in the genus Tuber. Some truffle species usually grow in an area devoid of vegetation, called brûlé, which knowledge about the microbial composition and structure is still limited. Here, we investigated the bacterial and fungal communities of Tuber indicum ascocarps and soils inside and outside a characteristic brûlé from a poplar plantation with no truffle production history in northeastern China using a high-throughput sequencing approach. A predominance of bacterial phylum Proteobacteria was observed in all samples, with Bacillus among the main genera in the ascocarps, while members of Lysobacter and unidentified Acidobacteria were more abundant in the soil. In addition, Gibberella, Fusarium and Absidia were the dominant fungi in the ascocarps, while Tuber were enriched in the ascocarps and soils inside the brûlé. Soil samples from inside the brûlé had a lower bacterial diversity and a greater fungal diversity than did those from outside the brûlé. Furthermore, some mycorrhization helper bacteria (Rhizobium) and ectomycorrhiza-associated bacteria (Lysobacter) were detected, indicating their potential roles in the complex development of underground fruiting bodies and brûlé formation. These findings may contribute to the protection and cultivation of truffles.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Microbial Communities of Ascocarps and Soils in a Natural Habitat of Tuber Indicum

Wang

Guo

et al. 2021

Preprint

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“…associated with Thamnidium elegans is in line with this trend. Diverse Bacillus bacteria were found living with truffles ( Barbieri et al, 2005 ; Perlińska-Lenart et al, 2020 ). Paenibacillus has been reported from Laccaria bicolor ( Bertaux et al, 2003 ), Sebacina vermifera ( Sharma et al, 2008 ), and is known to produce pre-symbiotic and symbiotic interactions with Glomus ( Bidondo et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

et al. 2021

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Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component.

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“…associated with Thamniudium elegans is in line with this trend. Diverse Bacillus bacteria were found living with truffles [109,110]. Paenibacillus has been reported from Laccaria bicolor [111], Sebacina vermifera [112] , and is known to produce pre-symbiotic and symbiotic interactions with Glomus [113] .…”

Section: Discussionmentioning

confidence: 99%

Metabolic potential, ecology and presence of endohyphal bacteria is reflected in genomic diversity of Mucoromycotina

Muszewska

Okrasińska

Steczkiewicz

et al. 2020

Preprint

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We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e. Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e. U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e. M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e. Thamnidium elegans and Mucor saturninus). We complement genome analyses with a description of their digestive capabilities, their cell wall carbohydrate composition, and total lipid profiles. Finally, we link the presence of endohyphal bacteria with observed characteristics.One of the genomes, Thamnidium elegans, harbours a complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to remaining isolates regardless of their evolutionary distance. T. elegans has expanded carbon assimilation capabilities particularly efficiently degrades carboxylic acids, has a higher diacylglycerol: triacylglycerol ratio and phospholipid composition suggesting a more rigid cellular membrane. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several differences particularly in their carbon source preferences and encoded carbohydrate repertoire. All tested Mucoromycotina shares features including the ability to produce 18:3 gamma-linoleic acid and fucose as a cell wall component.Author SummaryIn our paper, we report on the genomic sequences of six Mucoromycotina strains and an associated bacterium from Paenibacillus genus. Mucoromycotina are often studied in pathogenic context albeit their basic biology remains understudied. This manuscript expands on the collection of currently sequenced Mucorales and Umbelopsidales, including the first sequenced Thamnidium isolate, which was sequenced together with a Paenibacillus bacterium. The interaction with a bacterial partner alters the metabolism, cell membrane composition but not the exoskeleton of the fungus. The associated bacterium provided multiple enzymes that significantly expanded the digestive capabilities of the fungal host. Parallel sequencing and phenotyping of Mucorales and Umbelopsidales enabled us to look at the differences of both lineages within Mucoromycotina. We demonstrate that the predicted digestive capabilities are in line with experimental validation. Based on the cell wall composition data and genomic underpinnings of carbohydrate metabolism we were able to confirm the universal presence of fucose in Mucoromycotina cell walls. Fatty acid, phospholipid and acylglycerol composition support the usage of 18:3 gamma-linoleic acid as a chemotaxonomic marker of Mucoromycotina and corroborate TAG as a dominant storage lipid in these organisms.Genomic features, digestive capabilities, fatty acid composition differ between Mucorales and Ubelopsidales pointing at subtle but significant changes in the course of Mucoromycotina radiation.

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Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.)

Cited by 30 publications

References 71 publications

Microbial Communities of Ascocarps and Soils in a Natural Habitat of Tuber Indicum

Microbial Communities of Ascocarps and Soils in a Natural Habitat of Tuber Indicum

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

Metabolic potential, ecology and presence of endohyphal bacteria is reflected in genomic diversity of Mucoromycotina

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