Identification of Bacteria Associated with a Periodontal Disease in Thai Patients Based on Next-Generation Sequencing

Payungporn, Sunchai; Arirachakaran, Pratanporn; Poomipak, Witthaya; Praianantathavorn, Kesmanee; Charalampakis, Georgios; Poovorawan, Yong

doi:10.5812/jjm.13646

Cited by 6 publications

(5 citation statements)

References 34 publications

(43 reference statements)

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“…Several ASVs are more abundant in the supragingival biofilm of periodontitis subjects, such as Tannerella forsythia, Fusobacterium nucleatum subsp. vincentii, Porphyromonas endodontalis, Campylobacter gracilis, Eikenella corrodens, Leptotrichia hongkongensis, Desulfobulbus HMT 041, and Treponema amylovorum were previously associated with periodontal pockets [1,10,42,47,[52][53][54].…”

Section: Discussionmentioning

confidence: 98%

Oral Dysbiosis in Severe Forms of Periodontitis Is Associated With Gut Dysbiosis and Correlated With Salivary Inflammatory Mediators: A Preliminary Study

Kawamoto

Borges

Ribeiro

et al. 2021

Front. Oral. Health

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Inflammation is a driven force in modulating microbial communities, but little is known about the interplay between colonizing microorganisms and the immune response in periodontitis. Since local and systemic inflammation may play a whole role in disease, we aimed to evaluate the oral and fecal microbiome of patients with periodontitis and to correlate the oral microbiome data with levels of inflammatory mediator in saliva.Methods: Nine patients with periodontitis (P) in Stage 3/Grade B and nine age-matched non-affected controls (H) were evaluated. Microbial communities of oral biofilms (the supra and subgingival from affected and non-affected sites) and feces were determined by sequencing analysis of the 16SrRNA V3–V4 region. Salivary levels of 40 chemokines and cytokines were correlated with oral microbiome data.Results: Supragingival microbial communities of P differed from H (Pielou's evenness index, and Beta diversity, and weighted UniFrac), since relative abundance (RA) of Defluviitaleaceae, Desulfobulbaceae, Mycoplasmataceae, Peptostreococcales-Tissierellales, and Campylobacteraceae was higher in P, whereas Muribaculaceae and Streptococcaceae were more abundant in H. Subgingival non-affected sites of P did not differ from H, except for a lower abundance of Gemellaceae. The microbiome of affected periodontitis sites (PD ≥ 4 mm) clustered apart from the subgingival sites of H. Oral pathobionts was more abundant in sub and supragingival biofilms of P than H. Fecal samples of P were enriched with Acidaminococcus, Clostridium, Lactobacillus, Bifidobacterium, Megasphaera, and Romboutsia when compared to H. The salivary levels of interleukin 6 (IL-6) and inflammatory chemokines were positively correlated with the RA of several recognized and putative pathobionts, whereas the RA of beneficial species, such as Rothia aeria and Haemophilus parainfluenzae was negatively correlated with the levels of Chemokine C-C motif Ligand 2 (CCL2), which is considered protective. Dysbiosis in patients with periodontitis was not restricted to periodontal pockets but was also seen in the supragingival and subgingival non-affected sites and feces. Subgingival dysbiosis revealed microbial signatures characteristic of different immune profiles, suggesting a role for candidate pathogens and beneficial organisms in the inflammatory process of periodontitis.

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Section: Discussionmentioning

confidence: 98%

Oral Dysbiosis in Severe Forms of Periodontitis Is Associated With Gut Dysbiosis and Correlated With Salivary Inflammatory Mediators: A Preliminary Study

Kawamoto

Borges

Ribeiro

et al. 2021

Front. Oral. Health

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“…One of the major species in this niche is P. gingivalis, which is a well-known and well-studied pathogen associated with periodontitis [20]. P. catoniae, in contrast, was isolated mostly in control subjects [21][22][23]. The genus Porphyromonas has been described in various other body sites including: lungs [11,24], gut [25][26][27], skin [28,29], stomach [10,30], vagina [31] and nose [10,32].…”

Section: The Genus Porphyromonasmentioning

confidence: 99%

“…Studies investigating the oral microbiome in periodontitis compared to healthy controls also found this contrast between P. gingivalis and POTG. P. catoniae was reported to be a member of the healthy oral microbiome, and P. gingivalis as a member of the core microbiome in case of periodontitis [20][21][22][23].…”

Section: Oral Diseasesmentioning

confidence: 99%

Porphyromonas: A neglected potential key genus in human microbiomes

2021

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“…matruchotii KCTC 19325 isolated from human dental plaque to discover new antibacterial metabolites. This strain was initially discovered in 1925 and recognized as the most predominant strain in dental plaque on tooth surfaces. − AntiSMASH analysis on this bacterial genome revealed that there is a BGC for lanthipeptide, the same class of molecule as cebulantin previously discovered by the bioactivity-HiTES method (Table S1). As a result, we identified imidafenacin-induced antibacterial metabolites and also found that C.…”

Section: Introductionmentioning

confidence: 75%

Characterization of Chemical Interactions between Clinical Drugs and the Oral Bacterium, Corynebacterium matruchotii, via Bioactivity-HiTES

Lee,

Kim,

Lee

et al. 2024

ACS Chem. Biol.

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In the field of natural product research, the rediscovery of already-known compounds is one of the significant issues hindering new drug development. Recently, an innovative approach called bioactivity-HiTES has been developed to overcome this limitation, and several new bioactive metabolites have been successfully characterized by this method. In this study, we applied bioactivity-HiTES to Corynebacterium matruchotii, the human oral bacterium, with 3120 clinical drugs as potential elicitors. As a result, we identified two cryptic metabolites, methylindole-3-acetate (MIAA) and indole-3-acetic acid (IAA), elicited by imidafenacin, a urinary antispasmodic drug approved by the Japanese Pharmaceuticals and Medical Devices Agency (PMDA). MIAA showed weak antibacterial activity against a pulmonary disease-causing Mycobacterium conceptionense with an IC 50 value of 185.7 μM. Unexpectedly, we also found that C. matruchotii metabolized fludarabine phosphate, a USFDA-approved anticancer drug, to 2fluoroadenine which displayed moderate antibacterial activity against both Bacillus subtilis and Escherichia coli, with IC 50 values of 8.9 and 20.1 μM, respectively. Finally, acelarin, a prodrug of the anticancer drug gemcitabine, was found to exhibit unreported antibacterial activity against B. subtilis with an IC 50 value of 33.6 μM through the bioactivity-HiTES method as well. These results indicate that bioactivity-HiTES can also be applied to discover biotransformed products in addition to finding cryptic metabolites in microbes.

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Identification of Bacteria Associated with a Periodontal Disease in Thai Patients Based on Next-Generation Sequencing

Abstract: Background: Oral diseases depend on the relationship between host and various species of a bacterial community. The propagation of pathogenic bacteria within the mouth can cause periodontitis.

Cited by 6 publications

References 34 publications

Oral Dysbiosis in Severe Forms of Periodontitis Is Associated With Gut Dysbiosis and Correlated With Salivary Inflammatory Mediators: A Preliminary Study

Oral Dysbiosis in Severe Forms of Periodontitis Is Associated With Gut Dysbiosis and Correlated With Salivary Inflammatory Mediators: A Preliminary Study

Porphyromonas: A neglected potential key genus in human microbiomes

Characterization of Chemical Interactions between Clinical Drugs and the Oral Bacterium, Corynebacterium matruchotii, via Bioactivity-HiTES

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