Identification of Bacteria Contaminating Musca domestica (Diptera: Muscidae) Collected from Animal Husbandries

Abstract

Background: House flies (Musca domestica L.) may mechanically transmit many microorganisms to humans. Objectives: To identify bacteria contaminating Musca domestica (Diptera: Muscidae) collected from animal husbandries in province of Qom, Iran in the year of 2019. Methods: In the cross-sectional study, house flies were captured by plastic water bottle fly traps and insect nets from four districts of the Qom Province and were immediately transferred to the Department of Medical Entomology, School of Public Health in sterile glass containers, individually. The bacterial isolation of the surface and gut contents of the flies were separately performed by biochemical methods. To confirm Salmonella spp. and Shigella spp., antigen-antibody agglutination reactions were carried out by specific antisera. Results: A total of 23 bacterial species were identified from 160 house fly external surfaces and/or digestive tract were isolated. The most frequently isolated bacteria from the external body and the digestive tract of 160 flies were E. coli (73.8%) and P. aeruginosa (100%), respectively. The most frequently isolated bacteria were P. aeruginosa, Salmonella serogroup D, S. dysenteriae, E. coli, C. freundii, S. aureus, and S. epidermidis. Conclusions: House flies are important in the mechanical transmission of a different range of the pathogens. In addition, because of the geographical features of Qom and isolated pathogens, it seems that the possibility of mechanical disease transmission by the flies can be increased.

Pathogenic Bacteria Isolated from House Flies and Compared with Pathogenic Bacteria Isolated from Children with Diarrhea

“…This agrees with Hussny et al [12] at Thi-Qar University, College of Science in Iraq Kababian et al [14], in Iran this study in Qom Province; Central Iran, Hemmatinezhad et al [17] in Iran and, all studies had a greater percentage of bacterial contamination in the digestive system than in the external surface. This shows that the inner surface may be most crucial for vectoring a wide range of ambient as well as harmful microorganisms.…”

“…Baker et al [13]In Iraq; at the University of Tikrit; the house flies are the source of the vector of contamination in bacterial infections; where the study showed the isolation of 9 types of bacteria which are harmful to people. Kababian et al [14], This research was conducted in Central Iran's Qom Province .Because the location and manner of collecting the flies, as well as the same health system and collection times, are all to blame for the results' convergence.…”

Comparing the Bacterial Flora of Insectary and Filed Strains of Supella longipalpa (Blattaria: Ectobiidae) and their Antibiotic Resistant Pattern in Qom Province, Central Iran

“…Therefore, it can be explained that the bacteria isolated from the two samples in the current research can be considered as food-borne pathogens in the different wards of the hospitals. Similarly, a previous study conducted by our team showed E. coli and P. aeruginosa were the most prevalent bacterial isolates detected in houseflies ( Musca domestica ) ( 41 ).…”

Ecology of zoophilic flies in livestock biocenoses of Ukraine

“…Musca autumnalis De Geer, 1776 may act as the source of trichostrongylate distribution. Muscina stabulans (Fallén, 1817) and Stomoxys calcitrans (Linnaeus, 1758) are known to be mechanical carriers of Ancylostoma caninum (Ercolani, 1859) larvae and Trichuris vulpis (Frölich, 1789) eggs respectively (Paliy et al, 2018;Kababian et al, 2020). The species diversity and the number of zoophilic flies vary depending on the type and age of potential hosts (vertebrates).…”