Identification of Bacteria Indigenous Selected Rawa Pening Lake Owns Best Degradation Capability of the Organophosphate Pesticides (Malathion and Profenofos)

Isworo, Slamet; Purwanto, Purwanto; Sabdono, Agus

doi:10.3923/ajaps.2016.41.52

Cited by 1 publication

(3 citation statements)

References 6 publications

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“…The tools and materials used is: Water sampler, test tube, petri dish, winkler tube, ose needle, bunsen, beaker glass, funnel, separating funnel, erlenmeyer, measuring cup, spatula, volume pipette, propipet, filter paper, hot plate, vortex, magnetic stirrer, autoclave, distillers, ovens, shakers, incubators, refrigerators, electric balances, analytical balances, desiccators, incubators and spectrophotometers [12]…”

Section: Tools and Materialsmentioning

confidence: 99%

“…Centrifuge again at 12,000 rpm for 10 minutes. The supernatant containing DNA was transferred into a new Eppendorf tube and ready for the DNA amplification process" [12]. (5'-TACGGYTACCTTGTTACGACTT-3') were used for the 16S rDNA PCR (30).…”

Section: Identification Of Bacteria Based On 16s-rrna Gene Analysismentioning

confidence: 99%

“…Agarose, TAE1x Buffer, Extracted DNA, EtBr. Forward and reverse specific primer, Green Go Taq Master Mix[12].…”

mentioning

confidence: 99%

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Bioremediation Tofu Liquid Waste Based on Chemical Oxygen Demand (COD) Parameters

Isworo

Oetari

Prabowo

et al. 2022

ARRB

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Background and Objective: The majority of liquid waste generated by the tofu industry in Semarang exceeds the recommended limit. This condition disturbs the ecology of the surrounding environment. so a solution is needed to manage it. The use of bacteria in bioremediation technology has been proven to reduce pollutants produced by the tofu industry. The purpose of this study was to identify specific bacteria and test their ability to bioremediate pollutants from tofu wastewater using the Chemical Oxygen Demand (COD) parameter. Methodology: Methodology: The study was exploratory and experimental in nature, with the variable ability of degradative bacteria degrading tofu industrial wastewater based on COD parameters. Bacterial identification was performed using the 16S-rRNA Gene Analysis method, genomic DNA extraction with the Quick-DNA Bacterial Miniprep Kit, PCR amplification, and bi-directional sequencing. Results: The results of the identification of the selected bacteria show that they are molecules of the type nucleic acid with a query length of 1394. with a lineage report and a 93% phylogenetic tree as Bacillus subtilis, Bioremediation test shows that tofu waste can be degraded. The degradation percentage is 17%. Conclusion: Bacillus subtillis, a bacterium that degrades tofu industrial wastewater, was found as an indigenous bacteria.

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Section: Tools and Materialsmentioning

confidence: 99%

Section: Identification Of Bacteria Based On 16s-rrna Gene Analysismentioning

confidence: 99%

See 1 more Smart Citation

Bioremediation Tofu Liquid Waste Based on Chemical Oxygen Demand (COD) Parameters

Isworo

Oetari

Prabowo

et al. 2022

ARRB

View full text Add to dashboard Cite

show abstract

Identification of Bacteria Indigenous Selected Rawa Pening Lake Owns Best Degradation Capability of the Organophosphate Pesticides (Malathion and Profenofos)

Cited by 1 publication

References 6 publications

Bioremediation Tofu Liquid Waste Based on Chemical Oxygen Demand (COD) Parameters

Bioremediation Tofu Liquid Waste Based on Chemical Oxygen Demand (COD) Parameters

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