Identification of Bacteria Isolated from an Oligotrophic Lake with Pesticide Removal Capacities

López, Lilia; Pozo, Clementina; Rodelas, B.; Calvo, C.; Juárez, Belén; Martı́nez-Toledo, M.V.; González‐López, Jesús

doi:10.1007/s10646-003-6367-y

Cited by 71 publications

(36 citation statements)

References 29 publications

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“…The purported abilities of various Aeromonas spp. include heavy-metal reduction, oil biodegradation (by producing surfactant to emulsify hydrocarbons, thereby facilitating degradation by a microbial consortium) (47), pesticide removal (63), and selenite reduction (45,83). In addition to the efflux transporters mentioned above, other CDSs in the genome may encode enzymes for the alteration of toxic compounds and facilitate the environmental stability of A. hydrophila.…”

Section: Vol 188 2006mentioning

confidence: 99%

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

et al. 2006

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Section: Vol 188 2006mentioning

confidence: 99%

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

et al. 2006

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“…This fact confers substantial interest to the genus as a potential model system for the investigation of attributes that may correlate with adaptation and evolution of organisms to diverse thermal regimes (Vishnivetskaya et al, 2009). Several studies have shown that some strains of Exiguobacterium possess unique properties of interest for applications in biotechnology, bioremediation, industry and agriculture (Pattanapipitpaisal et al, 2002;Lopez et al, 2005;Okeke et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

Ordoñez

Lanzarotti

Kurth

et al. 2015

Front. Environ. Sci.

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Arsenic exists in natural systems in a variety of chemical forms, including inorganic arsenite (As [III]) and arsenate (As [V]). The majority of living organisms have evolved various mechanisms to avoid occurrence of arsenic inside the cell due to its toxicity. Common core genes include a transcriptional repressor ArsR, an arsenate reductase ArsC, and arsenite efflux pumps ArsB and Acr3. To understand arsenic resistance we have performed arsenic tolerance studies, genomic and bioinformatic analysis of two Exiguobacterium strains, S17 and N139, from the high-altitude Andean Lakes. In these environments high concentrations of arsenic were described in the water due to a natural geochemical phenomenon, therefore, these strains represent an attractive model system for the study of environmental stress and can be readily cultivated. Our experiments show that S17 has a greater tolerance to arsenite (10 mM) than N139, but similar growth in arsenate (150 mM). We sequenced the genome of the two Exiguobacterium and identified an acr3 gene in S17 as the only difference between both species regarding known arsenic resistance genes. To further understand the Acr3 we modeled the 3D structure and identified the location of relevant residues of this protein. Our model is in agreement with previous experiments and allowed us to identify a region where a relevant cysteine lies. This Acr3 membrane efflux pump, present only in S17, may explain its increased tolerance to As(III) and is the first Acr3-family protein described in Exiguobacterium genus.

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“…natatoria and M. luteus are commonly isolated from moist environments. M. luteus is environmentally ubiquitous and is found in biofilms of aquatic ecosystems (8,35), in soil (54), and on human and animal skin (17,29). Cells of M. luteus are gram positive, coccus shaped, arranged in clusters of tetrads, and nonmotile.…”

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Coaggregation by the Freshwater Bacterium Sphingomonas natatoria Alters Dual-Species Biofilm Formation

Min

Rickard

2009

Appl Environ Microbiol

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Coaggregation is hypothesized to enhance freshwater biofilm development. To investigate this hypothesis, the ability of the coaggregating bacterium Sphingomonas natatoria to form single-and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. Attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. Under static and flowing conditions, coaggregating S. natatoria 2.1gfp cells adhered to glass surfaces to form diaphanous single-species biofilms. When glass surfaces were precoated with coaggregation partner Micrococcus luteus 2.13 cells, S. natatoria 2.1gfp cells formed densely packed dual-species biofilms. The addition of 80 mM galactosamine, which reverses coaggregation, mildly reduced adhesion to glass but inhibited the interaction and attachment to glass-surface-attached M. luteus 2. In nature, most biofilms are not composed of one bacterial species but instead contain multiple species (24). These multispecies communities can be responsible for the fouling of ships (9, 44), the corrosion of liquid-carrying vessels (3, 14), and chronic infections in higher organisms (41,42,57). Recent research has demonstrated that in order for multispecies biofilm communities to develop, interbacterial communication is often essential (62) and facilitates the coordination of bacterial activities to promote the formation and to maintain the integrity of multispecies biofilm communities (28,32,60). Interspecies communication can be mediated by chemical or physical means. Mechanisms for chemical communication between different species include the secretion and uptake of metabolic by-products (11, 19), the exchange of genetic material (40), and the production and recognition of interspecies signal molecules such as short peptides (36) and autoinducer-2 (10). Mechanisms for interspecies physical communication can involve cell surface structures such as flagella or fimbriae (31, 48) and also include nonspecific adhesion between bacterial species (5) as well as highly specific coaggregations mediated by lectin-saccharide interactions (48).Coaggregation, the highly specific recognition and adhesion of different bacterial species to one another, was first discovered to occur between human oral bacteria in 1970 (23). Since then, research has shown that coaggregation occurs between specific bacterial species in environments other than the human oral cavity (48). Coaggregation interactions have been detected between bacteria isolated from canine dental plaque (21), the crop of chickens (61), the human female urogenital tract (30), the human intestine (34), and wastewater and freshwater biofilms (27,37,53). In particular, Buswell et al. (8) first demonstrated that coaggregation occurred between 19 freshwater strains that were isolated from a drinking water biofilm. Further studies by Rickard et al. demonstrated that coaggregation between these 19 strains was mediated by growth-phasedependent lectin...

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Identification of Bacteria Isolated from an Oligotrophic Lake with Pesticide Removal Capacities

Cited by 71 publications

References 29 publications

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

Coaggregation by the Freshwater Bacterium Sphingomonas natatoria Alters Dual-Species Biofilm Formation

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Identification of Bacteria Isolated from an Oligotrophic Lake with Pesticide Removal Capacities

Cited by 71 publications

References 29 publications

Genome Sequence of Aeromonas hydrophila ATCC 7966 T : Jack of All Trades

Genome Sequence of Aeromonas hydrophila ATCC 7966 T : Jack of All Trades

Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

Coaggregation by the Freshwater Bacterium Sphingomonas natatoria Alters Dual-Species Biofilm Formation

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Product

Resources

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Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades