Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase

Abstract

-A bacterial isolate producing lipase, named S31, was isolated from soil and identified as a strain of Burkholderia cepacia. S31 produced high activity of lipase which reached a maximum of 226.1 U/ml by fermenting at 30 °C for 60 h under the induction of olive oil. After purification, the lipase showed a single band of about 35 kDa in SDS-PAGE. The optimum temperature of the lipase was 70 °C and the optimum pH was 9.0. S31 lipase was stable at 40-70 °C and pH 0.5-10.0, as well as in several organic solvents, such as methanol, n-hexane, n-butanol, toluene and ethyl acetate. The presence of some metal ions (Ca 2+ , Mn 2+ , K + , Na + and Mg 2+ ) could activate the enzyme whereas Fe 2+ and Cu 2+ were found to be inhibitory. The lipase could cleave all of the three ester bonds of triglycerides. We conclude that S31 lipase is an alkaline lipase with a variety of highly desirable characteristics for research and industrial application.

An organic solvent and thermally stable lipase from Burkholderia ambifaria YCJ01: Purification, characteristics and application for chiral resolution of mandelic acid

“…Lipase YCJ01 remained 100% and 80% in 25% ethanol and acetonitrile for 30 days (data not shown), while only 43% and 71% residual activity of the lipase from B. cepacia S31 remained for 6 h at the same concentration of 25% ethanol and acetonitrile [9]. The lipase B. cepacia ATCC 25416 retained 89.9%, 61.6% and 52.7% residual activity in 30% methanol, ethanol and isopropanol after incubation for 2 h [22].…”

“…Some lipases, such as that from Bacillus sp. J33 [8], B. cepacia S31 [9], and Acinetobacter sp. RAG-1 [10], have been reported as possessing solvent-tolerance as well as thermostability.…”

Lipases and biosurfactants production by the newly isolated Burkholderia sp.

“…Other studies show that, in generally, bacterial lipases have neutral or alkaline pH optima and are stable over a wide pH range (Gupta et al, 2004;Hasan et al, 2006). The diverse optimal reaction temperature for lipase activity of different Burkholderia strains were reported: 37 °C (Ma et al, 2010), 55 °C (Liu et al, 2006Yang et al, 2007), 60 °C (Liu et al, 2006 and 70 °C (Lu et al, 2009).…”

Alkaliphilic bacteria: applications in industrial biotechnology

“…EH28 and was utilized for the synthesis of a flavoring substance involving organic solvents [5]. Lipase from Burkholderia cepacia S31 functioned optimally at pH 9.0 and temperature of 70°C [158]. It exhibited pH stability (pH 5.0-10.0) and retained excellent activity in many organic solvents.…”