Identification of Bacterial Community Composition in Freshwater Aquaculture System Farming of Litopenaeus vannamei Reveals Distinct Temperature-Driven Patterns

Abstract

Change in temperature is often a major environmental factor in triggering waterborne disease outbreaks. Previous research has revealed temporal and spatial patterns of bacterial population in several aquatic ecosystems. To date, very little information is available on aquaculture environment. Here, we assessed environmental temperature effects on bacterial community composition in freshwater aquaculture system farming of Litopenaeus vannamei (FASFL). Water samples were collected over a one-year period, and aquatic bacteria were characterized by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rDNA pyrosequencing. Resulting DGGE fingerprints revealed a specific and dynamic bacterial population structure with considerable variation over the seasonal change, suggesting that environmental temperature was a key driver of bacterial population in the FASFL. Pyrosequencing data further demonstrated substantial difference in bacterial community composition between the water at higher (WHT) and at lower (WLT) temperatures in the FASFL. Actinobacteria, Proteobacteria and Bacteroidetes were the highest abundant phyla in the FASFL, however, a large number of unclassified bacteria contributed the most to the observed variation in phylogenetic diversity. The WHT harbored remarkably higher diversity and richness in bacterial composition at genus and species levels when compared to the WLT. Some potential pathogenenic species were identified in both WHT and WLT, providing data in support of aquatic animal health management in the aquaculture industry.

Evidence of Increased Antibiotic Resistance in Phylogenetically-Diverse Aeromonas Isolates from Semi-Intensive Fish Ponds Treated with Antibiotics

“…Aeromonas salmonicida and Aeromonas veronii are widely known for their pathogenic attributes toward fish and humans, respectively (Robertson et al, 2014; Tang et al, 2014). A. salmonicida are widespread in non-salmonid fish (Wiklund and Dalsgaard, 1998); and are highly prevalent in skin ulcers in goldfish and pond-bred carp in Israel (Sinyakov et al, 2001).…”

Bacterial composition, abundance and diversity in fish polyculture and mussel-fish integrated cultured ponds in China

“…Understanding bacterial diversity becomes important to improve the application of microbial products as the native bacterial community can sway the abundance and function of any microbes added to the system. The microbial composition has been studied in the water column and sediment of shrimp ponds (Li, Zhang, Juck, Fortin, Greer & Tang ; Tang, Tao, Tan, Mu, Peng, Yang, Tong & Chen ; Zhang, Sun, Liu, Xuan, Jiang, Pan, Zhang, Gong, Lu, Yu, Kumar, Hu, Cao, Xue & Gong ), freshwater crab ponds (Liu, Zhou, He, Yao & Ringo ), seawater fish ponds (Zeng, Ma, Wei, Jiao, Tang, Wu & Jian ; Pereira, Salvador, Arrojado, Silva, Santos, Cunha, Gomes & Almeida ) and lobster tanks (Bourne, Young, Webster, Payne, Salmon, Demel & Hall ; Payne, Hall, Sly & Bourne ). However, knowledge in bacterial diversity in freshwater polyculture and integrated ponds is scarce (Zhou, Wang, Tang & Dai ; Huang, Liu, Li & Wang ).…”

Microbial metagenomics in aquaculture: a potential tool for a deeper insight into the activity

“…; Tang et al . ). Yeast biodiversity has been studied through the analysis of both 16S and 26S rDNA with the objective to obtain better probiotics for fish aquaculture (Navarrete & Tovar‐Ramírez ); 26S has also been used to study fungal communities (Heinrichs et al .…”

“…; Tang et al . ; Martínez‐Córdova et al . ); however, information regarding their unculturable microbial neighbours (i.e.…”