Identification of Baiying (Herba Solani Lyrati) commodity and its toxic substitute Xungufeng (Herba Aristolochiae Mollissimae) using DNA barcoding and chemical profiling techniques

Li, Ming; Au, Ka-Yee; Lam, Hilary; Cheng, Ling; Jiang, Ren‐Wang; But, Paul Pui‐Hay; Shaw, Pang‐Chui

doi:10.1016/j.foodchem.2012.06.049

Cited by 31 publications

(22 citation statements)

References 24 publications

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“…Therefore, the use of rbc L and mat K DNA barcodes regions as standard barcodes region together with ITS and psb A -trn H regions serving as complementary barcodes has helped tremendously in identifying herbal medicines and their adulterants [220]. Further standard DNA barcodes regions which have been used in authentication of HMP include trn L- trn F [125] and rpo C1 [146]. …”

Section: Dna Barcodingmentioning

confidence: 99%

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Abubakar

Salleh²,

Shamsir³

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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In the last two decades, there has been a tremendous increase in the global use of herbal medicinal products (HMPs) due to their claimed health benefits. This has led to increase in their demand and consequently, also, resulted in massive adulteration. This is due to the fact that most of the traditional methods cannot identify closely related species in a process product form. Therefore the urgent need for simple and rapid identification methods resulted in the discovery of a novel technique. DNA barcoding is a process that uses short DNA sequence from the standard genome for species identification. This technique is reliable and is not affected by external factors such as climates, age, or plant part. The difficulties in isolation of DNA of high quality in addition to other factors are among the challenges encountered using the DNA barcoding in the authentication of HMP. These limitations indicated that using DNA barcoding alone may ineffectively authenticate the HMP. Therefore, the combination of DNA barcoding with chromatographic fingerprint, a popular and generally accepted technique for the assessment and quality control of HMP, will offer an efficient solution to effectively evaluate the authenticity and quality consistency of HMP. Detailed and quality information about the main composition of the HMPs will help to ascertain their efficacy and safety as these are very important for quality control.

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Section: Dna Barcodingmentioning

confidence: 99%

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Abubakar

Salleh²,

Shamsir³

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…The PCR conditions for psbA-trnH were 30 cycles of denaturation at 94°C for 1 min, annealing at 55°C for 1 min, and extension at 72°C for 1.5 min. 14,[23][24][25] PCR products were separated and detected by 1.5% agarose gelelectrophoresis. Purified products were sequenced in both directions in a 3730XL sequencer (Applied Biosystems, U.S.A.).…”

Section: Sampling Of Plant Materialsmentioning

confidence: 99%

Comparative Analysis of DNA Barcoding and HPLC Fingerprint to Trace Species of Phellodendri Cortex, an Important Traditional Chinese Medicine from Multiple Sources

Zhang

et al. 2016

Biological & Pharmaceutical Bulletin

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Phellodendri Cortex is derived from the dried barks of Phellodendron genus species, has been extensively used in traditional Chinese medicine. The cortex is divided into two odorless crude drugs Guanhuangbo and Huangbo. Historically, it has been difficult to distinguish their identities due to a lack of identification methods. This study was executed to confirm the identity and to ensure the species traceability of Phellodendri Cortex. In the current study, analysis is based on the internal transcribed spacer (ITS) and psbA-trnH intergenic spacer (psbA-trnH) barcodes and HPLC fingerprint was carried out to guarantee the species traceability of Guanhuangbo and Huangbo. DNA barcoding data successfully identified the three plants of the Phellodendron genus species by ITS psbA-trnH, with the ability to distinguish the species origin of Huangbo. Moreover, the psbA-trnH data distinguished Guanhuangbo and Huangbo except to trace species. The HPLC fingerprint data showed that Guanhuangbo was clearly different from Huangbo, but there was no difference between the two origins of Huangbo. Additionally, the result of hierarchical clustering analysis, based on chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, palmatine and berberine, was consistent with the HPLC fingerprint analysis. These results show that DNA barcoding and HPLC fingerprint can discriminate Guanhuangbo and Huangbo. However, DNA barcoding is more powerful than HPLC fingerprint for species traceability in the identification of related species that are genetically similar. DNA barcoding is a useful scientific tool to accurately confirm the identities of medicinal materials from multiple sources. Key words Phellodendri Cortex; DNA barcoding; HPLC fingerprint; species traceabilityPhellodendri Cortex is derived from the dried barks of Phellodendron amurense RUPR., Phellodendron chinense SCHNEID. and the variant Phellodendron chinense var. glabriusculum SCHNEID. (Rutaceae).1) It is commonly used in traditional oriental medicine, and it has demonstrated a wide range of biological and pharmacological activities-antimicrobial, anti-inflammatory and anticancer-in numerous studies.2,3) In the Chinese Pharmacopoeia (2000 version), P. amurense and P. chinense are listed as the original plants of Phellodendri Cortex.4) However, P. amurense is listed as an endangered species and is strictly protected by law because this resource has decreased dramatically. 5) Additionally, there are differences between P. amurense and P. chinense regarding plant morphology and chemical composition. Thus, to control the quality of the herb and to protect the wild resource of P. amurense, Phellodendri Cortex is officially listed in the Chinese Pharmacopoeia (2005 version) as two different crude drugs, Guanhuangbo and Huangbo. 6) Guanhuangbo is derived from the P. amurense bark, while Huangbo is derived from the P. chinense bark. Since the wild resource of P. chinense is insufficient, the cultivated herb of P. chinense var. glabriusculum is also utilized in the production of crud...

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“…Four DNA barcodes (rbcL, matK, psbA-trnH, and ITS2) have been successfully used in the identification of many different species of phytomedicinals. 33,34 Plant DNA barcodes have been used efficiently to identify species richness in unknown floras, 35 identify traditional Tibetan medicinal plant Gentianopsis paludosa, 16 and discover complex plant groups of Indian Berberis species. 36 DNA barcoding system has many prospective uses not only in the identification but also in forensic science, verification of herbal medicines, and foodstuffs, resolving ambiguity of species in plant systematics.…”

Section: Application Of Dna Barcoding For the Analysis Of Phytomedicimentioning

confidence: 99%

“…The international trade in herbal products is a major force in the global economy and the demand is increasing in both developing and developed nations. 34 Recent reports indicate that herbal products available to consumers in the market place may be contaminated or substituted with alternative plant species and ingredient substitutions that are not listed on the labels. 17,54 A number of safety-related issues have emerged globally because of the inaccurate identification of herbal materials.…”

Section: Herbal Drug Authentication By Dna Barcodingmentioning

confidence: 99%

“…34,[55][56][57][58][59] Herbal product substitution has been reported for many individual plant species due to their morphological similarity like Echinacea sp., Chamomilla tincture, 60 Phyllanthus, 61,62 tea, 63 and nutraceuticals. 64,65 Although there is limited research available, the frequency of product mislabeling in herbal drugs has been estimated to be in the range from 14% to 33%.…”

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confidence: 99%

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DNA barcoding: a genomic-based tool for authentication of phytomedicinals and its products

Balachandran¹,

Saravanan²,

Ramalingam³

2015

BTAT

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DNA barcoding helps to identify the plant materials based on short, standardized gene sequences in a rapid, accurate, and cost-effective manner. Recent reports reveal that DNA barcoding can be used for the assignment of unknown specimens to a taxonomic group, authentic identification of phytomedicinals, and in plant biodiversity conservation. Research indicates that there is no single universal barcode candidate for identification of all plant groups. Hence, comparative analysis of plant barcode loci is essential for choosing a best candidate for authenticating particular medicinal plant genus/families. Currently, both chloroplast/nuclear regions are used as universal barcodes for the authentication of phytomedicinals. A recent advance in genomics has further enhanced the progress in DNA barcoding of plants by the introduction of high-throughput techniques like next generation sequencing, which has paved the way for complete plastome sequencing that is now termed as super-barcodes. These approaches could improve the traditional ethno-botanical and scientific knowledge of phytomedicinals and their safe use. Hence, current focus is on the investigation of phytomedicinals and herbal product integrity and authenticity through DNA barcoding with the goal of protecting consumers from potential health risks associated with product substitution and contamination.

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Identification of Baiying (Herba Solani Lyrati) commodity and its toxic substitute Xungufeng (Herba Aristolochiae Mollissimae) using DNA barcoding and chemical profiling techniques

Cited by 31 publications

References 24 publications

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

Comparative Analysis of DNA Barcoding and HPLC Fingerprint to Trace Species of Phellodendri Cortex, an Important Traditional Chinese Medicine from Multiple Sources

DNA barcoding: a genomic-based tool for authentication of phytomedicinals and its products

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