Identification of benzo[a]pyrene-induced cell cycle-associated alterations in MCF-7 cells using infrared spectroscopy with computational analysis

Distinguishing nuclei-specific benzo[a]pyrene-induced effects from whole-cell alterations in MCF-7 cells using Fourier-transform infrared spectroscopy

“…This observation is in agreement with documented observations in quiescent vs. exponential growth phase MCF-7 cells (Pang et al, 2012).…”

“…For instance, induction of apoptosis following exposure to toxic chemicals could explain such nuclei deformity (nuclear and cytoplasmic shrinkage, chromatin condensation, inter-nucleosomal DNA cleavage and plasma membrane blebbing) (Zelig et al, 2009). Cellular responses to B[a]P exposure is time, concentration and cell type dependent (Hockley et al, 2006;Pang et al, 2012). A positive trend observed in treated cells (see ESI Fig.…”

“…B[a]P and other PAHs, are capable of altering cell cycle processes including G 0 /G 1 -phase arrest or its evasion (Khan and Dipple, 2000). Cells at various stages in their cycle have been shown to respond differently to chemical exposures, especially to B [a]P (Hamouchene et al, 2011;Pang et al, 2012). These observations are significant towards understanding changes occurring in specific cell populations (e.g.…”

“…Infrared (IR) spectroscopy, including techniques such as Fourier-transform IR (FTIR) spectroscopy, has enhanced the study of cells and cell cycle processes, including observations at subcellular levels (Hammiche et al, 2005;Pang et al, 2012;Pijanka et al, 2009). Such biospectroscopy techniques provide a rapid, as well as a direct, alternative approach for analysing the cell, and a sensitive, reagent-free method for detecting intracellular changes, including within its subcellular components (Lipiec et al, 2014).…”

Co-exposure of C60 fullerene with benzo[a]pyrene results in enhanced biological effects in cells as determined by Fourier-transform infrared spectroscopy

“…In line with previous investigations, three relatively low doses were employed (B[a]P exposure concentrations at 10 -6 M, 10 -7 M and 10 -8 M; C60 at 0.1 mg/L, 0.01 mg/L and 0.001 mg/L). 32,33 Spectral alterations were associated with effects on AhR-inducible CYP1A1, DNA damage inferred by P21 WAF1/CIP1 and oxidative stress [measured by thiobarbituric acid reactive substances (TBARS) and levels of intracellular reactive oxygen species (ROS)]. This study aimed to gain insights into a binary exposure including a carbon-based NP and chemical contaminant.…”