Identification of bioactive compounds by GC-MS and α-amylase and α-glucosidase inhibitory activity of Rauvolfia tetraphylla L. and Oroxylum indicum (L.) Kurz: an in vitro and in silico approach

Swargiary, Ananta; Daimari, Manita

doi:10.1186/s40816-020-00219-3

Cited by 10 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…α-Amylase and α-glucosidase are two crucial metabolic enzymes linked to diabetes. Several plants and plant-derived phytocompounds are reported to possess α-amylase and α-glucosidase inhibitory properties [31,32] . The present study revealed considerable inhibitory properties of the crude extract of L. crustacea against α-amylase and α-glucosidase enzymes in in vitro experiments.…”

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Analysis and Antihyperglycemic Property of Lindernia crustacea (L.) F. Muell.

Roy¹,

Swargiary²,

Daimari³

2022

IJPS

Self Cite

View full text Add to dashboard Cite

Swargiary et al.: Antihyperglycemic Property of Lindernia crustaceaLindernia crustacea (L.) F. Muell is a small herbaceous plant with several ethnomedicinal values. The present study investigated the phytochemical content and alpha-amylase and alpha-glucosidase inhibitory property of Lindernia crustacea. Methanolic crude extract of plant was obtained following the Soxhlet method. The crude extract was studied for alpha-amylase and alpha-glucosidase inhibitory activity. Phytochemical analysis was carried out using the gas chromatography-mass spectrometry technique. Furthermore, docking study was carried out with the phytocompounds to see the binding affinity with the enzymes. In silico drug-likeness and pharmacological properties were also carried out using Swiss absorption, distribution, metabolism and excretion, and absorption, distribution, metabolism, excretion and toxicity lab tools. The plant extracts showed concentration-dependent inhibition of enzyme activities with half-maximal inhibitory concentration values of 3.11 mg/ml and 548.9 µg/ml for alpha-amylase and alphaglucosidase, respectively. Gas chromatography-mass spectrometry study identified ten phytocompounds with molecular weights ranging from 264.4 to 561 g/mol. Docking study showed 1-(4-Hydroxybenzoyl)-6,7-dimethoxyisoquinoline as the best binding compound with the enzymes. Phytocompounds identified from Lindernia crustacea were predicted to have substantial drug-likeness and absorption, distribution, metabolism, excretion and toxicity properties. The enzyme inhibition study and binding interactions of phytocompounds suggest promising alpha-amylase and alpha-glucosidase inhibitory activity of Lindernia crustacea. Therefore, the aerial part of Lindernia crustacea may be further investigated to know the exact mode of biological actions.

show abstract

Section: Resultsmentioning

confidence: 99%

Gas Chromatography-Mass Spectrometry Analysis and Antihyperglycemic Property of Lindernia crustacea (L.) F. Muell.

Roy¹,

Swargiary²,

Daimari³

2022

IJPS

Self Cite

View full text Add to dashboard Cite

show abstract

“…All the optimized compounds were saved in the mol2 format to carry out the molecular docking studies with the enzymes αamylase, α-glucosidase, and human pancreatic lipase. Acarbose was used as the reference inhibitor for α-amylase and α-glucosidase enzymes (Swargiary and Daimari, 2020), while methoxy undecyl phosphonic acid (MUP) and orlistat were used as the reference inhibitors for the human pancreatic lipase enzyme (Trang et al, 2017).…”

Section: Molecular Docking-ligand Preparationmentioning

confidence: 99%

“…Crystal structures of α-amylase (PDB:2QV4), α-glucosidase (maltase) (PDB: 2QMJ), and human pancreatic lipase (PDB:1LPB) were downloaded from the PDB database (http://www.rcsb.org/pdb). These crystal structures are crystallized with the acarbose ligand used as a reference inhibitor for α-amylase and α-glucosidase, while the MUP and orlistat ligands were used for human pancreatic lipase (Trang et al, 2017;Swargiary and Daimari, 2020). The information of the amino acids of the active site in the enzymes was used as a reference to carry out a targeted coupling in the catalytic sites in each of the crystallized inhibitors.…”

Section: Molecular Docking-ligand Preparationmentioning

confidence: 99%

“…The compounds that presented higher percentage of absorption according to their TPSA values were 4-HSA (90.24%), O4BBA (90.24%), 18-HA (89.15%), cyperine (88, 67%), TOPA (83.39%), azelaic acid (83.26%), and phthalic acid (83.26%). With the results of the evaluation of the pharmacological and toxicological properties, it was observed that those compounds that did not present any toxicological risk and any violation of Lipinski's rules were proposed as possible inhibitors of the enzymes to be evaluated; therefore, the compounds 2,5DHA, cyperin, diospyrol, hypoxyphenone, Frontiers in Pharmacology frontiersin.org lecanoric acid, orsellinic acid, prephenic acid, SDA, and O4BBA were those that were used for the in silico analysis to observe the behavior against these enzymes, comparing them with known inhibitors such as acarbose, orlistat, and MUP (Trang et al, 2017;Swargiary and Daimari, 2020).…”

Section: Prediction Of Pharmacokinetic and Toxicological Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distribution

Torres-Benítez,

Ortega-Valencia,

Jara-Pinuer

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

The objective of this research was to characterize the chemical composition of ethanolic extracts of the lichen species Placopsis contortuplicata, Ochrolechia frigida, and Umbilicaria antarctica, their antioxidant activity, and enzymatic inhibition through in vitro and molecular docking analysis. In total phenol content, FRAP, ORAC, and DPPH assays, the extracts showed significant antioxidant activity, and in in vitro assays for the inhibition of pancreatic lipase, α-glucosidase, and α-amylase enzymes, together with in silico studies for the prediction of pharmacokinetic properties, toxicity risks, and intermolecular interactions of compounds, the extracts evidenced inhibitory potential. A total of 13 compounds were identified by UHPLC-ESI-QTOF-MS in P. contortuplicata, 18 compounds in O. frigida, and 12 compounds in U. antarctica. This study contributes to the knowledge of the pool of bioactive compounds present in lichens of temperate and polar distribution and biological characteristics that increase interest in the discovery of natural products that offer alternatives for treatment studies of diseases related to oxidative stress and metabolic syndrome.

show abstract

“…Oroxylum indicum (L.) Kurz belongs to the family Bignoniaceae and is widely distributed throughout Asian counties, including Malaysia, Thailand, Indonesia, India, Vietnam, Philippines, China, Taiwan, and Japan. It is an important culinary herb with several medicinal and culinary purposes ( Swargiary and Daimari, 2020 ). The parts used in traditional medicinal practices are the fruit, seed, leaves, stems, bark and roots.…”

Section: Introductionmentioning

confidence: 99%

LC–TOF-MS/MS and GC-MS based phytochemical profiling and evaluation of wound healing activity of Oroxylum Indicum (L.) Kurz (Beka)

et al. 2022

View full text Add to dashboard Cite

Background: Beka (Oroxylum indicum (L.) Kurz) has been used as a culinary herb and natural remedy by the local communities in Malaysia. The leaf of O. indicum is traditionally used for the treatment of diarrhea, high blood pressure, and improving digestive health.Objectives: The present study was conducted to evaluate the phytochemical constituents and wound healing properties (in vitro and in vivo models) of aqueous and ethanol extracts of O. indicum leaves.Methods: The total phenolic (TPC) and total flavonoid (TFC) contents in the plant extracts were determined by the spectrophotometric methods. Further, the extract was characterized by Liquid Chromatography Time-of-Flight Mass Spectrometry (LC-TOF-MS/MS) and Gas Chromatography-Mass Spectrometry (GC-MS). The wound healing activity was assessed using the in vitro scratch wound-healing assay and in vivo excisional wound model.Results: The results show the ethanol leaves extract had the higher TPC (164 mg GAE/g) when compared with the aqueous leaves extract (30 mg gallic acid equivalents/g). The ethanol leaves extract was also found to have higher TFC (101 mg Catechin equivalents/g) than the aqueous leaves extract (76 mg Catechin equivalents/g). The ethanol leaves extract was then used for further chemical analysis. The LC–TOF-MS/MS analysis showed that the leaves extracts of O. indicum contains many important compounds such as Orientin, Chrysin, Pinoquercetin, Cupressuflavone, Puerarin xyloside, Forsythiaside and Paederoside. In GC-MS analysis, 19 compounds were identified in ethanolic leaves extract. The wound healing studies shows that O. indicum has promising wound healing activity by increasing the rate of wound contraction significantly (p < 0.05).Conclusion: In conclusion, the present study showed that O. indicum leaf contains important phytochemicals and the wound healing potential of the O. indicum extract may probably be as a result of the presence of various phytoconstituents.

show abstract

Identification of bioactive compounds by GC-MS and α-amylase and α-glucosidase inhibitory activity of Rauvolfia tetraphylla L. and Oroxylum indicum (L.) Kurz: an in vitro and in silico approach

Cited by 10 publications

References 40 publications

Gas Chromatography-Mass Spectrometry Analysis and Antihyperglycemic Property of Lindernia crustacea (L.) F. Muell.

Gas Chromatography-Mass Spectrometry Analysis and Antihyperglycemic Property of Lindernia crustacea (L.) F. Muell.

Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distribution

LC–TOF-MS/MS and GC-MS based phytochemical profiling and evaluation of wound healing activity of Oroxylum Indicum (L.) Kurz (Beka)

Contact Info

Product

Resources

About