Identification of bioactives from Astragalus chinensis L.f. and their antioxidant, anti-inflammatory and anti-proliferative effects

Hu, Ting; Liu, Qi-Mei; He, Xin; Huang, Fei; Zhang, Mingwei; Jiang, Jianguo

doi:10.1007/s13197-017-2902-3

Cited by 23 publications

(13 citation statements)

References 22 publications

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“…The NO inhibition activities of AR have already been reported previously. 33 Moreover, formononetin, 34 calycosin, 35 and soyasaponin I 36 have been reported to significantly inhibit the NO production; therefore, the active ingredients of both AR and HR were considered. Ononin, 37 astragaloside IV, 38 and isoastragaloside I 39 in AR and indolelactic acid 40 and afromosin 41 in HR have also reported the function in the NO production inhibition, which may be related to the anti-inflammatory activity of AR and HR.…”

Section: Anti-inflammatory Activity Of Ar and Hrmentioning

confidence: 99%

Metabolomic Determination of Specialized Metabolites Using Liquid Chromatography-Tandem Mass Spectrometry in the Traditional Chinese Medicines Astragali Radix and Hedysari Radix

Bai

Zheng

Han

et al. 2020

Natural Product Communications

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The Traditional Chinese Medicines (TCMs) Astragali Radix (AR) derived from Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao and A. membranaceus (Fisch.) Bge., and Hedysari Radix (HR) derived from Hedysarum polybotrys Hand.-Mazz. (family Leguminosae) are well-known for increasing the tonic effects on “Qi.” A better insight into the specialized (secondary) metabolites is essential to understand the effects of TCM; however, such metabolites remain largely unknown. Here, we performed a metabolomics-based analysis using liquid chromatography-tandem mass spectrometry in 3 plant tissues—periderm, phloem, and xylem—to identify potential bioactive metabolites. Multivariate statistical analysis revealed 29 metabolites showing a significant difference between groups and 10 biomarker candidates of AR and HR. An anti-inflammatory assay showed that the xylem of both AR and HR and the phloem of HR showed higher anti-inflammatory activity than the positive control quercetin in terms of nitric oxide inhibition.

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Section: Anti-inflammatory Activity Of Ar and Hrmentioning

confidence: 99%

Metabolomic Determination of Specialized Metabolites Using Liquid Chromatography-Tandem Mass Spectrometry in the Traditional Chinese Medicines Astragali Radix and Hedysari Radix

Bai

Zheng

Han

et al. 2020

Natural Product Communications

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“…Astragalin was shown to be cytotoxic to cultured human hepatocellular carcinoma HepG2 cells, but not human breast cancer cell line MCF-7 [57]. It also decreased cell viability and increased apoptosis of cultured human lung cancer cell lines (A549 and H1299), but not the normal (BEAS-2B) lung cells [58]. The mechanism for its selectivity toward tumor versus normal cells is unknown, but anti-oxidants can inhibit the growth of tumor cells through cell cycle arrest or apoptosis via depletion of reactive oxygen species because their growth is dependent on H 2 O 2 [14].…”

Section: Bioactive Compounds In Beetrootmentioning

confidence: 99%

Red Beetroot and Betalains as Cancer Chemopreventative Agents

Lechner

Stoner

2019

Molecules

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Carcinogenesis is the process whereby a normal cell is transformed into a neoplastic cell. This action involves several steps starting with initiation and followed by promotion and progression. Driving these stages are oxidative stress and inflammation, which in turn encompasses a myriad of aberrant gene expressions, both within the transforming cell population and the cells within the surrounding lesion. Chemoprevention of cancer with bioreactive foods or their extracted/purified components occurs via normalizing these inappropriate gene activities. Various foods/agents have been shown to affect different gene expressions. In this review, we discuss whereby the chemoprevention activities of the red beetroot itself may disrupt carcinogenesis and the activities of the water-soluble betalains extracted from the plant.

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“…Formononetin (FMN) is a bioactive compound, which is widely distributed in nature and has been reported to possess numerous pharmacological activities, including antitumor, oxygen free radical-scavenging, cell lipid peroxide-reducing and cholesterol-reducing effects (12)(13)(14)(15). In our preliminary experiments, it was demonstrated that, in a rat model, FMN could attenuate balloon-induced neointima formation and reduce platelet-derived growth factor (PdGF) levels…”

Section: Introductionmentioning

confidence: 93%

Formononetin protects against balloon injury‑induced neointima formation in rats by regulating proliferation and migration of vascular smooth muscle cells via the TGF‑β1/Smad3 signaling pathway

et al. 2018

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The present study investigated the effects of formononetin (FMN) against balloon injury‑induced neointima formation in vivo and platelet‑derived growth factor (PDGF)‑BB‑induced proliferation and migration of vascular smooth muscle cells (VSMCs) in vitro, and explored the underlying mechanisms. A rat model of carotid artery injury was established, in order to examine the effects of FMN on balloon injury‑induced neointima formation. Histological observation of the carotid artery tissues was conducted by hematoxylin and eosin staining. VSMC proliferation during neointima formation was observed by proliferating cell nuclear antigen staining. Subsequently, rat aortic VSMCs were isolated, and the effects of FMN on PDGF‑BB‑induced VSMC proliferation and migration were determined using Cell Counting Kit‑8 and Transwell/wound healing assays, respectively. Immunohistochemical and immunocytochemical staining was applied to measure the expression of transforming growth factor (TGF)‑β in carotid artery tissues and VSMCs, respectively. SMAD family member 3 (Smad3)/phosphorylated (p)‑Smad3 expression was examined by western blotting. FMN treatment significantly inhibited the abnormal proliferation of smooth muscle cells in neointima, and alterations to the vascular structure were attenuated. In addition, pretreatment with FMN effectively inhibited the proliferation of PDGF‑BB‑stimulated VSMCs (P<0.05). FMN also reduced the number of cells that migrated to the lower surface of the Transwell chamber and decreased wound‑healing percentage (P<0.05). The expression levels of TGF‑β were decreased by FMN treatment in vivo and in vitro, and Smad3/p‑Smad3 expression was also markedly inhibited. In conclusion, FMN significantly protected against balloon injury‑induced neointima formation in the carotid artery of a rat model; this effect may be associated with the regulation of VSMC proliferation and migration through altered TGF‑β1/Smad3 signaling.

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Identification of bioactives from Astragalus chinensis L.f. and their antioxidant, anti-inflammatory and anti-proliferative effects

Cited by 23 publications

References 22 publications

Metabolomic Determination of Specialized Metabolites Using Liquid Chromatography-Tandem Mass Spectrometry in the Traditional Chinese Medicines Astragali Radix and Hedysari Radix

Metabolomic Determination of Specialized Metabolites Using Liquid Chromatography-Tandem Mass Spectrometry in the Traditional Chinese Medicines Astragali Radix and Hedysari Radix

Red Beetroot and Betalains as Cancer Chemopreventative Agents

Formononetin protects against balloon injury‑induced neointima formation in rats by regulating proliferation and migration of vascular smooth muscle cells via the TGF‑β1/Smad3 signaling pathway

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