Identification of biofilm producing bacterial species causing dental caries and their sensitivity to zoocin A and clove hydroalcoholic extract

Khalili, Haniyeh; Sisakhtpour, Rouhollah; Abdollahzadeh, Sedigheh

doi:10.15171/ipp.2018.27

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Since non-responders possess a high abundance of this bacteria, its acidic products of metabolism along with its propensity to aggregate with other colonizers may contribute to resistance against the anti-bacterial properties of SDF. The species Granulicatella adiacens that was significantly higher in non-responders is frequently found in dental plaque, endodontic infection, and dental abscesses, and is also associated with other serious infections [ 37 ]. By comparing non-responders and SDF-treated subjects using LEfSe, in addition to Granulicatella , the genus Leptotrichia was more abundant in the plaque-infected site.…”

Section: Discussionmentioning

confidence: 99%

Microbial population shift and metabolic characterization of silver diamine fluoride treatment failure on dental caries

Paul

Sierra

et al. 2021

PLoS ONE

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The objective of this pilot study was to describe the microbial profiles present in the plaque and saliva of children who continued to develop new carious lesions following treatment with silver diamine fluoride (“nonresponders”) compared to caries active, caries-free, and children immediately receiving SDF treatment for untreated caries in order to identify potential microbial differences that may relate to a re-incidence of caries. Saliva and plaque samples from infected and contralateral sites were obtained from twenty children who were either caries free, had active carious lesions, were caries active and received SDF treatment immediately before sampling, or had previously received SDF treatment and developed new caries. In total, 8,057,899 Illumina-generated sequence reads from 60 samples were obtained. Reads were processed using the Quantitative Insights Into Microbial Ecology pipeline. Group differences were assessed using Analysis of Variance Models and Tukey Honest Significant Differences. To identify significant taxa between treatment groups, Linear discriminant analysis Effect Size (LefSe) and Analysis of Differential Abundance Taking Sample Variation Into Account were used. Differential abundant analysis indicated that members of the Lachnospiraceae family were significantly enriched in non-responders and the genus Tannerella and species Granulicatella adiances were also highly abundant in this group. LefSe analysis between non-responders and SDF-treated groups revealed that genera Leptotrichia and Granulicatella were enriched in non-responders. We observed the highest abundance of phosphotransferase system and lowest abundance of lipopolysaccharide synthesis in non-responders. The microbiome in dental biofilms is responsible for initiation and progression of dental caries. SDF has been shown to be effective in arresting the progression carious lesions, in part due to its antimicrobial properties. Findings suggest that the differential abundance of select microbiota and specific pathway functioning in individuals that present with recurrent decay after SDF treatment may contribute to a potential failure of silver diamine fluoride to arrest dental caries. However, the short duration of sample collection following SDF application and the small sample size emphasize the need for further data and additional analysis.

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Section: Discussionmentioning

confidence: 99%

Microbial population shift and metabolic characterization of silver diamine fluoride treatment failure on dental caries

Paul

Sierra

et al. 2021

PLoS ONE

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“…Since non-responders possess a high abundance of this bacteria, its acidic products of metabolism along with its propensity to aggregate with other colonizers may contribute to resistance against the anti-bacterial properties of SDF. The species Granulicatella adiacens that was significantly higher in non-responders is frequently found in dental plaque, endodontic infection, and dental abscesses, and is also associated with other serious infections [28]. By comparing non-responders and SDF-treated subjects using LEfSe, in addition to Granulicatella, the genus Leptotrichia was more abundant in the plaque-infected site.…”

Section: Discussionmentioning

confidence: 99%

Microbial Population Shift and Metabolic Characterization of Silver Diamine Fluoride Treatment Failure on Dental Caries

Paul

Sierra

et al. 2020

Preprint

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Objectives: The objective of this pilot study was to describe the microbial profiles present in the plaque and saliva of children who continued to develop new carious lesions following treatment with silver diamine fluoride (nonresponders) compared to caries active, caries- free and children with arrested caries following SDF treatment in order to identify potential microbial differences that may relate to a re-incidence of caries. Methods: Saliva and plaque samples from infected and contralateral sites were obtained from twenty children who were either caries free, had active carious lesions, were caries active and received SDF treatment immediately before sampling, or had previously received SDF treatment and developed new caries. In total, 8,057,899 Illumina-generated sequence reads from 60 samples were obtained. Reads were processed using the Quantitative Insights Into Microbial Ecology pipeline. Group differences were assessed using Analysis of Variance Models and Tukey Honest Significant Differences. To identify significant taxa between treatment groups, Linear discriminant analysis Effect Size (LefSe) and Analysis of Differential Abundance Taking Sample Variation Into Account were used. Results: Differential abundant analysis indicated that members of the Lachnospiraceae family were significantly enriched in non-responders and the genus Tannerella and species Granulicatella adiances were also highly abundant in this group. LefSe analysis between non- responders and SDF-treated groups revealed that genera Leptotrichia and Granulicatella were enriched in non-responders. We observed the highest abundance of phosphotransferase system and lowest abundance of lipopolysaccharide synthesis in non-responders. Discussion: The microbiome in dental biofilms is responsible for the progression of dental caries. SDF has been shown to be effective in arresting the progression carious lesions, thought to be related to its antimicrobial properties. Findings suggest that a differential abundance of select microbiota and specific pathway functioning may contribute to a potential failure of silver diamine fluoride to arrest dental caries.

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“…Chemical cure PMMA was selected to avoid thermal damage to the GO during other curing methods [10]. Streptococcus mutans was targeted in this study as it is one of the basic bacterial types that are able to form cariogenic plaque biofilms [32][33][34]. Testing of MIC for GO dispersion was done aiming to predict the optimal concentration of GO to be used with PMMA to produce antibacterial effect.…”

Section: Discussionmentioning

confidence: 99%

Incorporating nano graphene oxide to poly-methyl methacrylate; antibacterial effect and thermal expansion.

Gamal

Gomaa

Matrawy

2019

Journal of Modern Research

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This in vitro study was performed to evaluate antibacterial effect and thermal expansion of commercial available poly-methyl methacrylate (PMMA) incorporated by nano graphene oxide (GO) in different concentration. Method: Nano graphene oxide in five concentrations 0 %, 0.05 %, 0.1 %, 0.15 % and 1 % by weight was incorporated to PMMA to form groups I-V respectively. To evaluate the antibacterial effect against Streptococcus Mutans, minimal inhibitory concentration (MIC) for GO dispersion was done and agar well diffusion test for groups I-IV was done. Thermal expansion was assessed through measuring the linear coefficient of thermal expansion. Results: For antibacterial effect, all concentrations tested for MIC showed inhibition zone varied from 0.5 to 2mm in diameter. All tested groups evaluated by agar well diffusion method showed no inhibition zones except for group II. Significant reduction in coefficient of thermal expansion was showed by increasing GO concentration. Conclusions: Incorporation of GO in to PMMA at concentration of 0.5 % can inhibit the growth of Streptococcus Mutans. There is an inverse relation between the concentrations of GO and the coefficient of thermal expansion.

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Identification of biofilm producing bacterial species causing dental caries and their sensitivity to zoocin A and clove hydroalcoholic extract

Cited by 3 publications

References 16 publications

Microbial population shift and metabolic characterization of silver diamine fluoride treatment failure on dental caries

Microbial population shift and metabolic characterization of silver diamine fluoride treatment failure on dental caries

Microbial Population Shift and Metabolic Characterization of Silver Diamine Fluoride Treatment Failure on Dental Caries

Incorporating nano graphene oxide to poly-methyl methacrylate; antibacterial effect and thermal expansion.

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