Identification of biomarkers for lymph node metastasis in early-stage cervical cancer by tissue-based proteomics

Wang, W; Hl, Jia; Jm, Huang; Yc, Liang; H, Tan; Hz, Geng; Ly, Guo; Sz, Yao

doi:10.1038/bjc.2014.92

Cited by 63 publications

(48 citation statements)

References 38 publications

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“…The antibodies were listed in the Additional file 2: Table S6. The positive level of immunohistochemical staining was scored as described [26]. For statistical analysis, the IHC scores (ranging from 0 to 6) were evaluated and the staining score of 4 was defined as the cutoff.…”

Section: Methodsmentioning

confidence: 99%

Tripartite motif containing 62 is a novel prognostic marker and suppresses tumor metastasis via c-Jun/Slug signaling-mediated epithelial-mesenchymal transition in cervical cancer

Chen

Shang

et al. 2016

J Exp Clin Cancer Res

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BackgroundTRIM62 (tripartite motif containing 62) has been found to act as a tumor suppressor of several cancers. However, its precise biological role and related mechanism remain unknown in cervical cancer (CC).MethodsQuantitative Real-time PCR and western blot were adopted to detect the mRNA and protein expression level of TRIM62 in both human CC cell lines and tissues. Immunohistochemistry was used to measure the TRIM62 expression in 30 normal cervical and 189 CC tissues. Univariate and multivariate Cox regression analyses and Kaplan–Meier survival analyses performed to investigate the association between TRIM62 expression and CC patients’ prognosis. The effect of TRIM62 on CC growth and metastasis was studied in vitro and in vivo. Multi-pathway reporter array were utilized to identify the potential signaling manipulated by TRIM62.ResultsTRIM62 was frequently down-regulated in both human CC cells and tissues. Low expression of TRIM62 in CC tissues was associated with aggressive clinicopathological features of CC patients. In addition, TRIM62 was also an independent poor prognostic factor for overall and disease-free survival of CC patients after surgery. Moreover, enforced expression of TRIM62 in CC cells significantly inhibited their abilities of proliferation, migration and invasion in vitro. Besides, subcutaneous xenograft tumor model and xenograft mouse metastatic model respectively displayed that TRIM62 impeded the growth and metastasis of CC in vivo. Furthermore, mechanism study exhibited that TRIM62 could suppress epithelial-mesenchymal transition (EMT) by inhibiting c-Jun/Slug signaling. The inhibitory role of TRIM62 in tumor proliferation might be through regulating cell cycle related proteins CyclinD1 and P27 by targeting c-Jun.ConclusionTRIM62 is a potential prognostic biomarker in CC and suppresses metastasis of CC via inhibiting c-Jun/Slug signaling-mediated EMT.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-016-0445-5) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Tripartite motif containing 62 is a novel prognostic marker and suppresses tumor metastasis via c-Jun/Slug signaling-mediated epithelial-mesenchymal transition in cervical cancer

Chen

Shang

et al. 2016

J Exp Clin Cancer Res

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“…Proteomics analysis is currently considered to be a powerful tool for the global evaluation of protein expression and has been widely applied in studying biomarkers and the molecular pathogenesis of diseases [11].…”

Section: Introductionmentioning

confidence: 99%

Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms

Xie

Chu

et al. 2019

Cardiol J.

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“…SOD2 was chosen for validation as it has been previously linked to cervical carcinogenesis (26). Also, it has been documented as secreted by human hepatocytes (27).…”

Section: Resultsmentioning

confidence: 99%

High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases

Pappa

Kontostathi

Makridakis

et al. 2017

CGP

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Abstract. Background: Oncogenic infection by HPVThe vast majority of cervical cancer incidents are related to a group of 13 high-risk oncogenic human papilloma virus (HPV) types, classified according to sequence similarity, based on established criteria by the International Committee on the Taxonomy of Viruses (1). Types HPV16 and HPV18 represent the most common high-risk types causing cervical cancer (2, 3). Microabrasions on the cervical epithelium surface, allow the entry of HPV and the ensuing infection of the basal membrane epithelium. The initial overexpression of E6 and E7 HPV oncoproteins in the upper layers of the epithelium, leads to the production of viral particles and the establishment of productive infection, associated with the formation of low-grade squamous intraepithelial lesions (LSIL). Subsequent integration of the virus in the host cells, results in progression to high-grade squamous intraepithelial lesions (HSIL), leading eventually to cervical cancer (4-7).Proteomics technologies offer an holistic approach through the identification of many critical proteins, that could facilitate the understanding of biological mechanisms underlying cervical cancer pathogenesis. Proteomic tools have been utilized to study the mechanisms of action of drugs and the discovery of putative biomarkers for cervical cancer (8). However, in the above approaches, only the two-dimensional gel electrophoresis (2DE) coupled to MALDI-TOF has been utilized in most studies so far, whereas the LC-MS/MS technology, a very sensitive technology, has not been widely used. Furthermore, in these few studies where LC-MS/MS was applied, only the intracellular proteome was analyzed, 507

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Identification of biomarkers for lymph node metastasis in early-stage cervical cancer by tissue-based proteomics

Cited by 63 publications

References 38 publications

Tripartite motif containing 62 is a novel prognostic marker and suppresses tumor metastasis via c-Jun/Slug signaling-mediated epithelial-mesenchymal transition in cervical cancer

Tripartite motif containing 62 is a novel prognostic marker and suppresses tumor metastasis via c-Jun/Slug signaling-mediated epithelial-mesenchymal transition in cervical cancer

Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms

High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases

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