Identification of Biomarkers Modulating Osteogenic Differentiation With Immune and Inflammation Landscapes in Mesenchymal Stem Cells: A Bioinformatics-based Comprehensive Study

Abstract

Background: Inducing osteogenic mesenchymal stem cell (MSC) differentiation brings a huge potential for clinical bone defect repair. This present study is aimed to identify novel key biomarkers for osteogenesis of MSCs, and analyze their possible regulation roles on immune and inflammation during this process.Results: Seven datasets (GSE159137, GSE159138, GSE114117, GSE88865, GSE153829, GSE63754, GSE73087) were obtained from the gene expression omnibus database, and 200 differentially expressed genes (DEGs) were identified from the train datasets. Biomarkers were screened through Logical regression of the selection operator algorithm, whose expressions and diagnostic performances were verified in the test datasets. FKBP prolyl isomerase 5 (FKBP5), insulin like growth factor binding protein 2, prostaglandin E receptor 2, SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) and transmembrane O-mannosyltransferase targeting cadherins 1 were established to be osteogenesis-related biomarkers. Finally, the DEGs of immune and inflammation in osteogenesis were detected, and their correlation with the hub biomarkers were analyzed. The five selected biomarkers are strongly associated with immune and inflammation related genes.Conclusion: Taken together, our manuscript identified five biomarkers related to stem cells osteogenesis and discussed their potential perspectives in immunoregulation, inflammation and tissue engineering during the process.