Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples

Laurell, Henrik; Bouisson, M; Berthélemy, Philippe; Rochaix, Philippe; Déjean, Sébastien; Besse, Philippe; Susini, Christiane; Pradayrol, Lucien; Vaysse, Nicole; Buscail, Louis

doi:10.3748/wjg.v12.i21.3344

Cited by 68 publications

(36 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…182 The genetic profile of EUS-FNA samples has been analyzed through macro-array analysis that included 1,176 genes associated with cancer, by using commercially available complementary DNA samples. 183 A complex statistical analysis was performed (hierarchical clustering, principal component analysis, significance analysis of microarrays) that allowed initial establishment of the gene expression. The results were then validated through quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry.…”

Section: Molecular Markersmentioning

confidence: 99%

Role of endoscopic ultrasound in the diagnosis and staging of pancreatic cancer

Săftoiu

Vilmann

2008

J of Clinical Ultrasound

View full text Add to dashboard Cite

Early diagnosis of pancreatic cancer remains a difficult task, and multiple imaging tests have been proposed over the years. The aim of this review is to describe the current role of endoscopic ultrasound (EUS) for the diagnosis and staging of patients with pancreatic cancer. A detailed search of MEDLINE between 1980 and 2007 was performed using the following keywords: pancreatic cancer, endoscopic ultrasound, diagnosis, and staging. References of the selected articles were also browsed and consulted. Despite progress made with other imaging methods, EUS is still considered to be superior for the detection of clinically suspected lesions, especially if the results of other cross-sectional imaging modalities are equivocal. The major advantage of EUS is the high negative predictive value that approaches 100%, indicating that the absence of a focal mass reliably excludes pancreatic cancer. The introduction of EUS-guided fine needle aspiration allows a preoperative diagnosis in patients with resectable cancer, as well as a confirmation of diagnosis before chemoradiotherapy for those that are not. This comprehensive review highlighted the diagnostic capabilities of EUS including the newest refinements such as contrast-enhanced EUS, EUS elastography, and 3-dimensional EUS. The place of EUSguided biopsy is also emphasized, including the addition of molecular marker techniques.

show abstract

Section: Molecular Markersmentioning

confidence: 99%

Role of endoscopic ultrasound in the diagnosis and staging of pancreatic cancer

Săftoiu

Vilmann

2008

J of Clinical Ultrasound

View full text Add to dashboard Cite

show abstract

“…It was first isolated as a 25-kDa glycoprotein covalently bound with matrix metalloproteinase-9 in human neutrophils, and elevated tissue NGAL expression has been documented under diverse infectious and inflammatory conditions, such as inflammatory bowel disease, appendicitis, diverticulitis and urinary tract infections [3]. It has been extensively investigated as a biomarker for the early diagnosis of acute kidney injury from diverse etiologies [4], but recent evidence suggests that NGAL expression is induced in many types of human cancer, including breast [5], gastric [6], esophageal squamous cell [7], colorectal [8], pancreatic [9] and ovarian cancer [10]. In previous work, we have demonstrated that increased levels of NGAL appear to be a characteristic of polycythemia vera and essential thrombocythemia patients [11].…”

Section: Introductionmentioning

confidence: 99%

Imatinib Mesylate Therapy Induces Reduction in Neutrophil Gelatinase-Associated Lipocalin Serum Levels and Increase in Leptin Concentrations in Chronic Myeloid Leukemia Patients in Molecular Remission

Alonci

Allegra²,

Russo³

et al. 2011

Acta Haematol

View full text Add to dashboard Cite

The aim of the present study was to determine serum levels of neutrophil gelatinase-associated lipocalin (NGAL) and leptin in patients with chronic myeloid leukemia (CML) at diagnosis and after imatinib therapy when patients achieved a complete molecular remission. The study was conducted on 22 patients with CML in the chronic phase and 10 healthy subjects. The median serum NGAL levels in CML patients at diagnosis were significantly higher compared to age-matched controls. After imatinib therapy, all patients achieved complete molecular remission and NGAL levels decreased and were found significantly lower with respect to the baseline. No significant correlations were found between NGAL levels and other disease parameters. Before imatinib therapy, the median blood leptin levels were not significantly different from those of controls. After therapy with imatinib, all patients in molecular remission presented an increase in leptin levels. Future research is eagerly awaited as it may demonstrate the real role of NGAL and leptin in the onset and progression of CML.

show abstract

“…In most of the common human cancers, NGAL expression is upregulated, for example in colorectal cancer, gastric cancer (Kubben et al, 2007), esophageal squamous cell carcinoma (Zhang et al, 2007), lung adenocarcinoma (Friedl et al, 1999), primary liver carcinoma (Li et al, 2012), breast cancer (Fernández et al, 2005), and thyroid neoplasia (Iannetti et al, 2008), while in a few conditions, NGAL expression is downregulated or remains debatable, for example in pancreatic cancer (Furutani et al, 1998;Laurell et al, 2006;Tong et al, 2008), prostate cancer (Mahadevan et al, 2011), and chronic myeloid leukemia (Villalva et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of neutrophil gelatinase-associated lipocalin (NGAL) in colorectal cancer: a meta-analysis

Wang¹,

Zeng²

2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Growing evidence has implicated that neutrophil gelatinase-associated lipocalin (NGAL) plays a role in a spectrum of human cancers. Several observational studies from different parts of the world have been devoted to elucidate the clinical relationship between NGAL and colorectal cancer. This meta-analysis aimed to explore the overall accuracy of NGAL detection for the diagnosis and prognosis of colorectal cancer. Based on comprehensive literature screening on Pubmed, Ovid, and CNKI databases, our screening covered all published papers until March 2013. The relevant papers were selected according to some stringent inclusion criteria. Essential data were extracted from the recruited papers and further processed by systematic meta-analysis. The meta-analysis included 5 studies for diagnosis of colorectal cancer. Overall, the pooled sensitivity and specificity of all studies were 73% (95%CI = 0.69-0.76) and 89% (95%CI = 0.85-0.93). The pooled positive likelihood ratio and negative likelihood ratio were 5.41 (95%CI = 3.85-7.59) and 0.37 (95%CI = 0.22-0.62). The pooled diagnostic odds ratios was 18.05 (95%CI = 11.77-27.69). The area under the summary receiver operating characteristic curve for the diagnosis of colorectal cancer was 0.87. Meanwhile, 3 studies were included to evaluate the prognostic significance of NGAL overexpression in colorectal cancer patients. The pooled hazard ratio was 2.12 (95%CI = 1.35-3.33). High level of NGAL predicted poor disease-free survival. Thus, NGAL is a potential biomarker for the diagnosis and prognosis of colorectal cancer.

show abstract

Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples

Abstract: Expression profiling is a useful method to identify biomarkers and potential target genes. Molecular analysis of EUS-guided FNA samples in pancreatic cancer appears as a valuable strategy for the diagnosis of pancreatic adenocarcinomas.

Cited by 68 publications

References 34 publications

Role of endoscopic ultrasound in the diagnosis and staging of pancreatic cancer

Role of endoscopic ultrasound in the diagnosis and staging of pancreatic cancer

Imatinib Mesylate Therapy Induces Reduction in Neutrophil Gelatinase-Associated Lipocalin Serum Levels and Increase in Leptin Concentrations in Chronic Myeloid Leukemia Patients in Molecular Remission

Clinical significance of neutrophil gelatinase-associated lipocalin (NGAL) in colorectal cancer: a meta-analysis

Contact Info

Product

Resources

About