Identification of blaIMP-22 in Pseudomonas spp. in urban wastewater and nosocomial environments: biochemical characterization of a new IMP metallo-enzyme variant and its genetic location

Pellegrini, Cristina; Mercuri, Paola Sandra; Celenza, Giuseppe; Galleni, Moreno; Segatore, Bernardetta; Sacchetti, Elisa; Volpe, Roberto; Amicosante, Gianfranco; Perilli, Mariagrazia

doi:10.1093/jac/dkp061

Cited by 45 publications

(32 citation statements)

References 24 publications

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“…Exposure of wildlife to untreated wastewater may present a pathway for transfer of human-derived bacteria, and the antibiotic resistance genes they carry, to wildlife populations (Pellegrini et al 2009). Ultimately, this may lead to colonization by microorganisms atypical of the natural habitats of wildlife species (Power et al 2013).…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

Delport

Harcourt

Beaumont

et al. 2015

Journal of Wildlife Diseases

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ABSTRACT:Greater interaction between humans and wildlife populations poses significant risks of anthropogenic impact to natural ecosystems, especially in the marine environment. Understanding the spread of microorganisms at the marine interface is therefore important if we are to mitigate adverse effects on marine wildlife. We investigated the establishment of Escherichia coli in the endangered Australian sea lion (Neophoca cinerea) by comparing fecal isolation from wild and captive sea lion populations. Fecal samples were collected from wild colonies March 2009-September 2010 and from captive individuals March 2011-May 2013. Using molecular screening, we assigned a phylotype to E. coli isolates and determined the presence of integrons, mobile genetic elements that capture gene cassettes conferring resistance to antimicrobial agents common in fecal coliforms. Group B2 was the most abundant phylotype in all E. coli isolates (n537), with groups A, B1, and D also identified. Integrons were not observed in E. coli (n521) isolated from wild sea lions, but were identified in E. coli from captive animals (n516), from which class I integrases were detected in eight isolates. Sequencing of gene cassette arrays identified genes conferring resistance to streptomycin-spectinomycin (aadA1) and trimethoprim (dfrA17, dfrB4). Class II integrases were not detected in the E. coli isolates. The frequent detection in captive sea lions of E. coli with resistance genes commonly identified in human clinical cases suggests that conditions experienced in captivity may contribute to establishment. Identification of antibiotic resistance in the microbiota of Australian sea lions provides crucial information for disease management. Our data will inform conservation management strategies and provide a mechanism to monitor microorganism dissemination to sensitive pinniped populations.

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Section: Introductionmentioning

confidence: 99%

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

Delport

Harcourt

Beaumont

et al. 2015

Journal of Wildlife Diseases

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“…According to the sequences, the Pc promoter of this integron was a variant designated PcW TGM-10 , providing strong expression of the gene cassette array, but associated with weak integrase IntI activity (15). This could possibly explain the stable integron content observed during a 6-year period with a likely repetitive independent acquisition of the integron from environmental sources (7,21). The cassette array of this class 1 integron was unusual in two aspects: first, although the coexistence of different ␤-lactamases is frequent in MBL-carrying integrons, in this one, two neighboring gene cassettes encoded two different ␤-lactamases (VIM-2 and OXA-10), a situation similar to the one described for P. aeruginosa isolates harboring VIM-6 and OXA-10 in Asia (16); second, a remnant of the qacF gene, seldomly reported in integrons harboring MBL genes, could have a residual function in promoting transcription of adjacent genes, as already described for other qac genes (qacE⌬1 and qacH) (7,9,13).…”

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Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

Sardelić

Bedenić

Colinon-Dupuich

et al. 2012

Antimicrob Agents Chemother

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f One hundred sixty-nine nonreplicate imipenem-resistant Pseudomonas aeruginosa strains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried the bla VIM-2 gene located in a novel class 1 integron composed in its variable part of the bla VIM-2 -bla oxa-10 -⌬qacF-aacA4 genes. Metallo-␤-lactamase-producing strains belonged to sequence types ST235 and ST111.

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“…Several metallo-␤-lactamases, such as VIM-2 from P. pseudoalcaligenes (18) and P. aeruginosa (19) and IMP-22 from P. fluorescens (12), were recovered from urban water samples. The number of different class A ␤-lactamases with carbapenemase activity is still limited, and the occurrence of most class A carbapenemases is at present sporadic (24), except for KPC enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Carbapenemases (serine-or metallo-␤-lactamases) remain the most common mechanism of resistance to carbapenems in Gram-negative organisms (22,23). To date, most acquired metallo-␤-lactamase (MBL)-encoding genes (bla IMP or bla VIM variants) have been reported from Pseudomonas aeruginosa and very rarely from P. fluorescens (8,12). Class A carbapenemases are either chromosome encoded or plasmid encoded and remain rarely identified in Gram-negative organisms.…”

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confidence: 99%

Novel Ambler Class A Carbapenem-Hydrolyzing β-Lactamase from a Pseudomonas fluorescens Isolate from the Seine River, Paris, France

Girlich

Poirel

Nordmann

2010

Antimicrob Agents Chemother

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A Pseudomonas fluorescens isolate (PF-1) resistant to carbapenems was recovered during an environmental survey performed with water from the Seine River (Paris). It expressed a novel Ambler class A carbapenemase, BIC-1, sharing 68 and 59% amino acid identities with ␤-lactamases SFC-1 from Serratia fonticola and the plasmid-encoded KPC-2, respectively. ␤-Lactamase BIC-1 hydrolyzed penicillins, carbapenems, and cephalosporins except ceftazidime and monobactams. The bla BIC-1 gene was chromosomally located and was also identified in two other P. fluorescens strains isolated from the Seine River 3 months later.Pseudomonas fluorescens is a psychrotrophic bacterium that expresses a chromosomally encoded and inducible Ambler class C ␤-lactamase (10, 15). Carbapenemases (serine-or metallo-␤-lactamases) remain the most common mechanism of resistance to carbapenems in Gram-negative organisms (22,23). To date, most acquired metallo-␤-lactamase (MBL)-encoding genes (bla IMP or bla VIM variants) have been reported from Pseudomonas aeruginosa and very rarely from P. fluorescens (8, 12). Class A carbapenemases are either chromosome encoded or plasmid encoded and remain rarely identified in Gram-negative organisms. Indeed, several class A carbapenemases are chromosomally encoded (NMC-A, SFC-1, SME-1 to -3, IMI-1), with the exception of the emerging KPC ␤-lactamases, which are plasmid encoded (24). The chromosomal location of the bla SFC-1 gene could be the result of a horizontal gene transfer into an environmental Serratia fonticola isolate (5). The bla SME genes from Serratia marcescens are presumed to be chromosomal. Genes encoding NMC-A and IMI ␤-lactamases have been found sporadically, either in clinical or in environmental isolates of Enterobacter cloacae and Enterobacter asburiae from rivers (2). The bla NMC-A gene was found chromosomally located in several clinical isolates. The bla IMI-1 gene was chromosomally located, whereas the bla IMI-2 genes were identified on plasmids. The imiR-imi-2 gene tandem in E. cloacae and E. asburiae appeared to be flanked by transposable elements (2). Class A carbapenemases such as GES-2, GES-5, and KPC-2 have been recently reported from P. aeruginosa (16) but not from other Pseudomonas species.Here, we characterize a novel class A carbapenemase identified from a P. fluorescens environmental isolate. This identification occurred during a survey aimed to study the spread of multidrug-resistant Gram-negative organisms in the environment. MATERIALS AND METHODSBacterial strains and plasmids. P. fluorescens isolate PF-1 was recovered from a water sample from the Seine River in downtown Paris. Water samples (100 ml) were collected in sterile bottles in January 2009 and filtered through nitrocellulose membranes (0.45-m pore size; Millipore), and the filters were resuspended in 1 ml of sterile water. Aliquots (0.1 ml) were plated on MacConkey agar plates supplemented with imipenem (1 g/ml), whereas 10-fold dilutions (0.1 ml) were plated on antibiotic-free MacConkey agar plates for bacterial count...

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Identification of blaIMP-22 in Pseudomonas spp. in urban wastewater and nosocomial environments: biochemical characterization of a new IMP metallo-enzyme variant and its genetic location

Abstract: The finding of the bla(IMP-22) gene in P. fluorescens environmental strains and P. aeruginosa clinical isolate suggests the ongoing spread of bla(MBL) genes in several bacterial species and in different environments.

Cited by 45 publications

References 24 publications

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS INESCHERICHIA COLIISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA)

Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

Novel Ambler Class A Carbapenem-Hydrolyzing β-Lactamase from a Pseudomonas fluorescens Isolate from the Seine River, Paris, France

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