Identification of blood and wound isolates of C. canimorsus and C. cynodegmi using VITEK2 and MALDI-TOF

Zangenah, Salah; Özenci, Volkan; Boräng, Stina; Bergman, Peter

doi:10.1007/s10096-012-1606-x

Cited by 28 publications

(23 citation statements)

References 29 publications

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“…Capnocytophaga canimorsus strains can also be identified with MALDI‐TOF (matrix‐assisted laser desorption/ionization time‐of‐flight analyser): in one study, it was able to identify six of six blood C. canimorsus strains, 13 of 14 wound strains and two of two ATCC reference strains (Zangenah et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Capnocytophaga cynodegmi , a similar pathogen, may cause several infections, varying from a local wound infection to endocarditis and meningitis, and is more frequently found in the immunocompromised (Khawari et al., ). The clinical characteristics are similar to those of C. canimorsus meningitis and it may be hard to distinguish between C. cynodegmi and C. canimorsus microbiologically (Suzuki et al., ; Zangenah et al., ). As both respond to the recommended empirical antibiotic treatment used for meningitis (Brouwer et al., ), the exact subtyping is clinically less relevant.…”

Section: Discussionmentioning

confidence: 99%

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Capnocytophaga canimorsus Meningitis: Three Cases and a Review of the Literature

Samkar¹,

Brouwer²,

Schultsz³

et al. 2015

Zoonoses and Public Health

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Bacterial meningitis is a disease with a high morbidity and mortality. It may be caused by the zoonotic pathogen Capnocytophaga canimorsus, which is part of the commensal oral flora in dogs and cats. We report three cases of C. canimorsus meningitis in a nationwide cohort study of bacterial meningitis patients and performed a review of the literature. Three episodes of C. canimorsus meningitis were identified in three patients included in a nationwide cohort study from 2006 through 2014. The calculated annual incidence was 0.03 per million adults. When combined with the literature, 33 patients were identified of which 28 were male (85%). The median age was 63 years, and 13 (42%) were immunocompromised, which consisted of alcoholism in 7 (21%). Animal contact could be established in 29 of 30 patients (93%) and consisted of dog bites in 22 of 29 (76%). One patient died (3%) and 8 had neurological sequelae upon discharge (25%), most often hearing loss (n = 6, 19%). Capnocytophaga canimorsus meningitis is associated with dog bites. Although mortality is relatively low, survivors often have neurological sequelae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Capnocytophaga canimorsus Meningitis: Three Cases and a Review of the Literature

Samkar¹,

Brouwer²,

Schultsz³

et al. 2015

Zoonoses and Public Health

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“…C. canis was isolated from a human wound specimen, and is thus here defined as a clinical isolate. These isolates have been described in detail in previous studies from our group [26–28]. The genomes for all 24 strains were used in the bioinformatic analyses, whereas 16 strains were subjected to antibiotic susceptibility testing based on consistent and reproducible growth patterns.…”

Section: Methodsmentioning

confidence: 99%

Genomic analysis reveals the presence of a class D beta-lactamase with broad substrate specificity in animal bite associated Capnocytophaga species

Zangenah

Andersson

Özenci

et al. 2016

Eur J Clin Microbiol Infect Dis

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Capnocytophga canimorsus and Capnocytophga cynodegmi can be transmitted from cats and dogs to humans, and can cause a wide range of infections including wound infections, sepsis, or endocarditis. We and others recently discovered two new Capnocytophaga species, C. canis and C. stomatis, mainly associated with wound infections. The first-line treatment of animal bite related infections is penicillin, and in case of allergy, doxycycline and trimethoprim/sulfamethoxazole. However, there is a lack of antibiotic susceptibility patterns for animal bite associated Capnocytophaga species. Thus, we set out to study the antibiotic profiles against animal bite associated Capnocytophaga species isolated from wound and blood cultures after cat and dog bites and coupled the findings to whole genome sequencing data. A total of 24 strains were included in the study. Phenotypic analysis of antibiotic resistance was performed with E-tests. The web-based tool ‘Resfinder’ was used to identify resistance genes in the whole genome dataset. Two strains of C. cynodegmi and two strains of the recently discovered C. stomatis were resistant to penicillin (MIC > 24 mg/L) and cephalosporins (MIC > 24 mg/L), and three out of these strains also exhibited resistance to imipenem (MIC = 32 mg/L). Genomic analysis revealed that these strains carried a class D beta-lactamase gene, which has not previously been found in Capnocytophaga spp. A class D beta lactamase with broad substrate specificity was found in animal bite associated Capnocytophaga species, which could have important implications when treating wound infections after cat and dog bites. It also suggests that pet animal bacteria can harbour resistance genes with relevance for human infections.

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“…Costs and time-frames for 16S rRNA analyses may be substantial per isolate, depending on sequences and repetitions (Clarridge, 2004;Justesen et al, 2010). There are a number of available conventional diagnostic microbiological tools which were compared for their identification capacity and reliability with MALDI-TOF MS, mostly for clinically relevant bacteria (Risch et al, 2010;van Veen et al, 2010;Saffert et al, 2011;Alvarez-Buylla et al, 2012;El-Bouri et al, 2012;Fang et al, 2012;Meex et al, 2012;Zangenah et al, 2012;Caretto et al, 2013;Jamal et al, 2013;Almuzara et al, 2015, to name a few recent studies).…”

Section: Comparison With Conventional and Molecular Identification Prmentioning

confidence: 99%

Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS

Popović

Kazazić

Strunjak‐Perović

et al. 2017

Environmental Research

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Identification of bacteria in aquatic and environmental applications, for monitoring purposes and research, for health assessments and therapy considerations of farmed and free-living aquatic organisms, still relies on conventional phenotypic and biochemical protocols. Although molecular techniques based on DNA amplification and sequencing are finding ways into diagnostic laboratories, they are time-consuming, costly and difficult in the case of multiplex assays. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate proteomic method reliable for identification of unknown bacteria to the genus and species level. Upon extension of databases, it will certainly find its position in environmental sciences. The paper presents an overview of the principle of the method, its effectiveness in comparison with conventional and molecular identification procedures, and applicability on environmental and aquatic isolates, discussing its advantages and shortcomings, as well as possible future implementations.

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Identification of blood and wound isolates of C. canimorsus and C. cynodegmi using VITEK2 and MALDI-TOF

Cited by 28 publications

References 29 publications

Capnocytophaga canimorsus Meningitis: Three Cases and a Review of the Literature

Capnocytophaga canimorsus Meningitis: Three Cases and a Review of the Literature

Genomic analysis reveals the presence of a class D beta-lactamase with broad substrate specificity in animal bite associated Capnocytophaga species

Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS

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