Identification of Blueberry Varieties Using Random Amplified Polymorphic Dna Markers

Arce-johnson, P.; Ríos, Mónica Aguinaga; Zúñiga, Martha C.; Vergara, Erika

doi:10.17660/actahortic.2002.574.33

Cited by 5 publications

(3 citation statements)

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“…Aruna et al (1995) developed a cultivar hierarchical key for the identification of 19 rabbiteye cultivars based on 11 RAPD markers amplified from four RAPD primers (OPC12, OPC17, OPG09, and OPG12). Arce-Johnson et al (2002) used two RAPD primers to identify the five most important Chilean highbush cultivars. RAPD analysis can be used to identify many useful polymorphisms rapidly and efficiently, and has tremendous potential for cultivar identification (Lu et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers

Cho¹,

Park²,

Kim³

et al. 2017

horts

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Blueberry cultivars have traditionally been identified based on the evaluation of sets of morphological characters; however, distinguishing closely related cultivars remains difficult. In the present study, we developed DNA markers for the genetic fingerprinting of 45 blueberry cultivars, including 31 cultivars introduced from the United States Department of Agriculture. We obtained 210 random amplified of polymorphic DNA (RAPD) markers using 43 different primers. The number of polymorphic bands ranged from three (OPG-10 and OPQ-04) to eight (OPR-16), with an average of five. A cluster analysis performed with the unweighted pair group method using arithmetic averages produced genetic similarity values among the blueberry cultivars ranging from 0.53 to 0.85, with an average similarity of 0.68. A dendrogram clustered the 45 blueberry cultivars into two main clusters, with a similarity value of 0.65. Cluster I consisted of four rabbiteye cultivars (Pink Lemonade, Alapaha, Titan, and Vernon) and the Ashworth northern highbush cultivar. Cluster II consisted of 31 northern highbush cultivars, eight southern highbush blueberry cultivars, and Northland half-highbush blueberry cultivar. Fifty five RAPD fragments selected were sequenced to develop sequence-characterized amplified region (SCAR) markers, resulting in the successful conversion of 16 of 55 fragments into SCAR markers. An amplified polymorphic band has the same size as the RAPD fragment or smaller according to the primer combinations in the 16 SCAR markers. Among these markers, a combination of 11 SCAR markers provided sufficient polymorphisms to distinguish the blueberry cultivars investigated in this study. These newly developed markers could be a fast and reliable tool to identify blueberry cultivars.

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Section: Resultsmentioning

confidence: 99%

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers

Cho¹,

Park²,

Kim³

et al. 2017

horts

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“…RAPD markers were the first to be applied and were proven to be able to differentiate Vaccinium corymbosum L. and Vaccinium angustifolium Ait. cultivars by different authors (Cho et al, 2017;Burgher-Maclellan and Mackenzie, 2004;Arce-Johnson et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Allergen coding genes and miRNA-based DNA fingerprints in revealing differences among Vaccinium corymbosum L. and Vaccinium myrtillus L.

Žiarovská,

Ražná,

Urbanová

et al. 2023

JCEA

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DNA-based marker fingerprinting techniques are developed with the aim of their effective use in the identification or comparative studies of plant genomes. In the genus Vaccinium, DNA markers help resolve taxonomic relationships among the species included in it. In this study, the allergen coding genes and micro RNAs were used as DNA markers and applied for the characterization of the fingerprints of Vaccinium corymbosum L. and Vaccinium myrtilus L. Both techniques were found to be effective in distinguishing the analysed Vaccinium accessions and in the generating of polymorphism and in the case of Bet v 1 Based Amplicon Polymorphism and Profilin Based Amplicon Polymorphism, separated fingerprints were obtained for Vaccinium myrtilus L.

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“…(Goulao et al, 2001). As well, RAPD analysis has been successfully used in Vaccinium spp., mainly for cultivar identification and relatedness studies (Arce-Johnson et al, 2002;Aruna et al, 1995;Levi and Rowland, 1997;Polashock and Vorsa, 1997).…”

Section: Introductionmentioning

confidence: 99%

An Overview of RAPD Analysis to Estimate Genetic Relationships in Lowbush Blueberry

Burgher-MacLellan

MacKenzie

2004

Small Fruits Review

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Randomly amplified polymorphic DNA (RAPD) analysis, a simple dominant molecular marker technique, has been used extensively for cultivar identification and relatedness studies in many perennial woody species. Thus, this technique should provide genetic information for lowbush blueberry (Vaccinium angustifolium Ait.). Young leaves of lowbush blueberry from field clones with varying phenotype were collected for DNA extraction. Pre-screening of RAPD primers resulted in 11 polymorphic primers and 140 consistent RAPD fragments. Eight primers were selected as useful for our study, the fragments scored and the data analyzed with Genstat5 to calculate similarity, produce dendrograms and perform a principal coordinate analysis. The RAPD analysis was able to identify distinct field clones. Average genetic similarity among field clones was 68% reflecting expected genetic variation.

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Identification of Blueberry Varieties Using Random Amplified Polymorphic Dna Markers

Cited by 5 publications

References 0 publications

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers

Allergen coding genes and miRNA-based DNA fingerprints in revealing differences among Vaccinium corymbosum L. and Vaccinium myrtillus L.

An Overview of RAPD Analysis to Estimate Genetic Relationships in Lowbush Blueberry

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