Identification of Boerka Goats Raised in Bali by using Molecular Analysis of IGF1 (Insulin-Like Growth Factor 1) Gene

Abstract

The fulfillment of the lamb demand in Bali, Boerka goats was developed by the local government in 2019. Boerka goats resulting from a cross between male Boer breeds and female Kacang goats are categorized as meat goats and predicted to have good adaptation to unfavorable environments like the Kacang goats and have good meat quality like Boer goats as their parents. The study aims to analyze insulin-like growth factor 1 (IGF1) as a gene related to the growth of Boerka goats. A total of 16 Boerka goats from a group of livestock farmers in Sanda village were used as objects of study. The blood samples were taken in a venoject tube filled with EDTA anticoagulant. DNA total was extracted from each sample and subjected to PCR with IFG-1 gene target by using IGF677F 5'-ATTACAAAGCTGCCTGCCCC-'3 and IGF879R 5'-ACCTTACCCGTATGAAAGGAATATACGT-'3 primers. PCR products were then sequenced and analyzed with the MEGA 11 program. Results of the study showed the IFG-1 gene of Boerka goats compose of nucleotides dominated by adenine base guanine (22.3%), and pyrimidine tyrosine (33.9%). In conclusion, Boerka goats raised in Bali genetically share a clade with Ovis aries and Capra malabari with a bootstrap value is 99% as a type of meat and milk goats so that Boerka goats tend to have characteristics and appearance resembling Ovis aries and Capra malabari goats.