Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Oliveira, Thais Cristina de Assis; Brito, Maria Aparecida Vasconcelos Paiva; Marval, Marcia Giambiagi-de; Vicentini, Nívea Maria; Lange, C. C.

doi:10.1017/s0022029921000595

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Brazil, for the identification of 380 bacteria isolated from milk samples from bovine patients with mastitis, MALDI-TOF MS showed a typing rate of 95.5%, and the accuracy for identifying Staphylococcus isolates was 93.2% [55].…”

Section: Resultsmentioning

confidence: 99%

Prevalence and sensitivity of contagious and environmental cow mastitis-causing pathogens to antibiotics in Ukrainian farms

Zaritskyi,

Zhuk,

Dreval

et al. 2024

Potr. S. J. F. Sci.

View full text Add to dashboard Cite

Mastitis is considered the most common and problematic disease, resulting in significant economic losses due to reduced milk yields, reduced quantity and quality of milk, treatment costs, and premature culling of animals. One of the traditional methods of treating mastitis in cows is using antibiotics, which leads to the emergence of polyresistant strains of microorganisms, the so-called Superbugs. The emergence of Superbugs, which are not sensitive to most existing antibiotics, is a major concern in veterinary and humane medicine. This study aimed to identify pathogens isolated from the secretion of the mammary gland of cows with mastitis to determine their spread and sensitivity to antibiotics. The samples of secretion from the udder were examined by bacteriological method. The isolates were identified by conventional methods and by the modern method – mass spectrometry (MALDI-TOF MS). The sensitivity of the bacterial isolates to antibiotics was determined by the disc diffusion method (Kirby-Bauer). The results of studies of cow mammary gland secretion samples indicate that 49.2% of the isolates are contagious, and 50.8% are environmental ones. The most common among the isolates of mastitis-causing pathogens were Streptoccocus аgalactiae – 16.9%, Streptococcus uberis – 10.9%, Staphylococcus aureus – 10.7%, E. Coli – 9.6%, Corynebacterium bovis – 7.3%, Staphylococcus haemolyticus – 4.8%, Staphylococcus chromogenes – 3.6%, Streptococcus dysgalactiae – 3.4%. Mastitis is caused by algae and yeast – 1% of all detected pathogens, respectively. According to the results of the determination of the sensitivity of isolates of mastitis-causing pathogens to antibiotics, it was found that most isolates were sensitive to Amoxicillin, Ceftiofur, and Rifampicin, and least of them – to Neomycin, Tylosin, Tilmicosin, Bacitracin.

show abstract

Section: Resultsmentioning

confidence: 99%

Prevalence and sensitivity of contagious and environmental cow mastitis-causing pathogens to antibiotics in Ukrainian farms

Zaritskyi,

Zhuk,

Dreval

et al. 2024

Potr. S. J. F. Sci.

View full text Add to dashboard Cite

show abstract

“…S. aureus and Streptococcus spp. are the most common etiologic agents involved in bovine mastitis [ 194 , 195 ]. In recent years, the emergence of resistance and the increasingly strict regulations on dairy farms regarding the use of these drugs in animal production has forced the development of alternatives, such as bacteriocins, for the control and prevention of this disease [ 196 ].…”

Section: Applications Of Bacteriocin-producing Staphylococc...mentioning

confidence: 99%

Comprehensive Approaches for the Search and Characterization of Staphylococcins

et al. 2023

View full text Add to dashboard Cite

Novel and sustainable approaches are required to curb the increasing threat of antimicrobial resistance (AMR). Within the last decades, antimicrobial peptides, especially bacteriocins, have received increased attention and are being explored as suitable alternatives to antibiotics. Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria as a self-preservation method against competitors. Bacteriocins produced by Staphylococcus, also referred to as staphylococcins, have steadily shown great antimicrobial potential and are currently being considered promising candidates to mitigate the AMR menace. Moreover, several bacteriocin-producing Staphylococcus isolates of different species, especially coagulase-negative staphylococci (CoNS), have been described and are being targeted as a good alternative. This revision aims to help researchers in the search and characterization of staphylococcins, so we provide an up-to-date list of bacteriocin produced by Staphylococcus. Moreover, a universal nucleotide and amino acid-based phylogeny system of the well-characterized staphylococcins is proposed that could be of interest in the classification and search for these promising antimicrobials. Finally, we discuss the state of art of the staphylococcin applications and an overview of the emerging concerns.

show abstract

“…25% of samples recovered. Oliveira et al [33] used MALDI-TOF measurements to identify 380 bacteria isolates from cases of bovine mastitis in Brazil. This survey identified various Streptococcus, Enterococcus, Lactococcus, and Aerococcus species as major players infecting animals.…”

Section: Mycoplasmamentioning

confidence: 99%

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Thompson

2022

Vet World

View full text Add to dashboard Cite

Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a valuable laboratory tool for rapid diagnostics, research, and exploration in veterinary medicine. While instrument acquisition costs are high for the technology, cost per sample is very low, the method requires minimal sample preparation, and analysis is easily conducted by end-users requiring minimal training. Matrix-assisted laser desorption ionization-time-of-flight MS has found widespread application for the rapid identification of microorganisms, diagnosis of dermatophytes and parasites, protein/lipid profiling, molecular diagnostics, and the technique demonstrates significant promise for 2D chemical mapping of tissue sections collected postmortem. In this review, an overview of the MALDI-TOF technique will be reported and manuscripts outlining current uses of the technology for veterinary science since 2019 will be summarized. The article concludes by discussing gaps in knowledge and areas of future growth.

show abstract

Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Cited by 5 publications

References 12 publications

Prevalence and sensitivity of contagious and environmental cow mastitis-causing pathogens to antibiotics in Ukrainian farms

Prevalence and sensitivity of contagious and environmental cow mastitis-causing pathogens to antibiotics in Ukrainian farms

Comprehensive Approaches for the Search and Characterization of Staphylococcins

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Contact Info

Product

Resources

About