Identification of Bovine Serum Albumin as an IgE-Reactive Beef Component in a Dog with Food Hypersensitivity against Beef

Ohmori, Kitaro; Masuda, Kenichi; Kawarai, Shinpei; Yasuda, Nobutaka; Sakaguchi, Masahiro; Tsujimoto, Hajime

doi:10.1292/jvms.69.865

Cited by 19 publications

(29 citation statements)

References 11 publications

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“…In this study, an increase in beef-specific IgE was detected in 5 of the 13 dogs with FA, 5 of the 7 dogs with CAD, and 1 of the 5 healthy dogs, and no other food allergens were detected with such high frequency. This relatively high frequency of beef-specific IgE detection might also be associated with vaccination, as previously reported [18,19]. Because beef-specific IgE can be found in dogs receiving vaccination, it is difficult to diagnose beef allergy with the IgE test alone.…”

Section: Discussionsupporting

confidence: 52%

“…In this study, we found no relationship between the clinical diagnosis of FA and the identification of food allergens using the IgE test. It has been reported that IgE production against beef components occurred in dogs that received vaccination because the vaccine products can contain fetal bovine serum [18,19]. In this study, an increase in beef-specific IgE was detected in 5 of the 13 dogs with FA, 5 of the 7 dogs with CAD, and 1 of the 5 healthy dogs, and no other food allergens were detected with such high frequency.…”

Section: Discussionmentioning

confidence: 43%

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Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy

Fujimura

Masuda

Hayashiya

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Two different allergy tests, antigen-specific immunoglobulin E quantification (IgE test) and flow cytometric analysis of antigen-specific proliferation of peripheral lymphocytes (lymphocyte proliferation test), were performed to examine differences in allergic reactions to food allergens in dogs with food allergy (FA). Thirteen dogs were diagnosed as FA based on clinical findings and elimination diet trials. Seven dogs clinically diagnosed with canine atopic dermatitis (CAD) were used as a disease control group, and 5 healthy dogs were used as a negative control group. In the FA group, 19 and 33 allergen reactions were identified using the serum IgE test and the lymphocyte proliferation test, respectively. Likewise, in the CAD group, 12 and 6 allergen reactions and in the healthy dogs 3 and 0 allergen reactions were identified by each test, respectively. A significant difference was found between FA and healthy dogs in terms of positive allergen detection by the lymphocyte proliferation test, suggesting that the test can be useful to differentiate FA from healthy dogs but not from CAD. Both tests were repeated in 6 of the dogs with FA after a 1.5-to 5-month elimination diet trial. The IgE concentrations in 9 of 11 of the positive reactions decreased by 20-80%, whereas all the positive reactions in the lymphocyte proliferation test decreased to nearly zero (P<0.05), suggesting that lymphocytes against food allergens may be involved in the pathogenesis of canine FA.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 43%

Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy

Fujimura

Masuda

Hayashiya

et al. 2011

The Journal of Veterinary Medical Science

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“…5 In a previous report, IgG and muscle phosphoglucomutase were identified as major cross-reactive allergens in dogs with food allergy that had detectable serum allergen-specific IgE to beef, lamb and cow's milk. 6 Bovine serum albumin (Bos d 6) also has been reported to be an allergen in beef-allergic dogs 7 and, given its abundance in cow's milk, 8 this evolutionarily conserved serum albumin could be an additional source of cross-reactivity between these two foods.…”

Section: Introductionmentioning

confidence: 99%

Serum IgE cross‐reactivity between fish and chicken meats in dogs

Bexley

Kingswell

Olivry

2018

Veterinary Dermatology

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Background In humans, a cross‐reactive clinical allergy has been reported between three chicken and fish meat proteins: beta‐enolase, aldolase A and parvalbumin. Objective To evaluate if IgE cross‐reactivity between chicken and fish also existed in the dog. Animals Sera from dogs with suspected allergic skin disease and with IgE against chicken and fish. Methods and materials Sera were analysed by ELISA and immunoblotting with chicken, white fish (haddock and cod) and salmon extracts. Reciprocal inhibition ELISAs and inhibition immunoblots were then performed. Protein sequencing of bands identified on multiple extracts was determined by mass spectrometry. Results Out of 53 archived canine sera tested by ELISA against chicken, white fish or salmon, 15 (28%), 12 (23%) and 26 (49%), respectively, had elevated IgE against one, two or all three of these extracts. Seven of the triple‐reactive sera were subjected to reciprocal inhibition ELISAs. A >50% inhibition was found between chicken–fish, chicken–salmon and fish–salmon in seven, four and five of seven dogs, respectively. Immunoblotting identified multiple IgE‐binding proteins of identical molecular weights in the three extracts; these were partially to fully cross‐reactive by inhibition immunoblotting. Mass spectrometry identified nine cross‐reactive proteins as: pyruvate kinase, creatine kinase, alpha‐actin, glyceraldehyde‐3‐phosphate dehydrogenase, beta‐enolase, aldolase, malate dehydrogenase, lactate dehydrogenase and triose‐phosphate isomerase 1. All of these have been reported previously as fish, shellfish and/or chicken allergens for humans. Conclusions and clinical importance Whether any of these newly identified IgE cross‐reactive chicken–fish allergens is the cause of clinical allergy needs to be determined in dogs reacting to at least two of these common food sources.

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“…Immunoblotting to detect allergen-specific IgE was performed using a published procedure with modifications. 10 Membranes were rinsed once in deionized water followed by PBS containing 0.1% Tween 20 (PBST). Blocking was performed by submersing the membrane in a 1% solution of cold water fish gelatin (Sigma-Aldrich; St Louis, MO, USA) in PBST (blocking buffer) and agitating on an orbital shaker for 1 h at room temperature.…”

Section: Ige Immunoblottingmentioning

confidence: 99%

“…These studies have identified as putative food allergens in dogs: pyruvate kinase M 1/2, Enolase 3, M-type creatine kinase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase a and triose phosphate isomerase 1 in chicken; bconglycinin in soybean; phosphoglucomutase, the bovine IgG heavy chain, and (to date unidentified) bands of 42, 37, 33, 31 and 27 kDa in milk; ovomucoid and ovalbumin in egg; and the IgG heavy chain and bovine serum albumin in beef. [9][10][11][12][13] Many of these allergens have been identified using archived sera (or pools of archived sera) preselected for IgE reactivity against the food source of interest, often with no clinical information available. Furthermore, this reactivity has generally been determined relative either to healthy dogs, or to dogs pre-selected as nonreactive against the food sources.…”

Section: Introductionmentioning

confidence: 99%

Serum IgE and IgG responses to dietary antigens in dogs with and without cutaneous adverse food reactions

Pucheu‐Haston

Mougeot²

2019

Veterinary Dermatology

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Background It is suspected that many canine cutaneous adverse food reactions (CAFR) are true immunological hypersensitivities; however, few specific dietary allergens have been identified. Objective To compare serum immunoglobulin (Ig)E and IgG reactivity to specific food antigens in privately owned dogs with and without CAFR. Animals Eighteen adult dogs with nonseasonal pruritus recruited from a hospital population. Methods and materials Dogs were fed an extensively hydrolysed poultry‐based diet exclusively for 12 weeks. Serum was collected at the beginning of the trial. Canine atopic dermatitis extent and severity index and pruritus Visual Analog Scale scoring were performed at the beginning and end of the trial. Immunoblotting was performed to identify IgE and/or IgG binding to specific proteins in beef, egg, milk, chicken, pork, soy and wheat extracts. Results A CAFR (defined as an unequivocal relapse of pruritus after dietary challenge) was diagnosed in 10 dogs, with 60% relapsing when fed chicken‐based diets. Binding of subjects’ IgG to almost all proteins in all extracts was seen regardless of reported dietary history. Few proteins were exclusively or predominantly bound by IgE in CAFR dogs. Exceptions included a 42 kDa band (chicken), a 52 kDa band (beef), a 46 kDa band (beef and milk) and a poorly defined high molecular weight protein or proteins (beef and milk). Conclusion This study demonstrated three protein bands and a poorly defined band predominantly recognized by sera from dogs with CAFR relative to non‐CAFR dog sera. Almost all proteins were bound by IgG in all dogs, suggesting prior exposure to unreported foods.

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Identification of Bovine Serum Albumin as an IgE-Reactive Beef Component in a Dog with Food Hypersensitivity against Beef

Cited by 19 publications

References 11 publications

Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy

Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy

Serum IgE cross‐reactivity between fish and chicken meats in dogs

Serum IgE and IgG responses to dietary antigens in dogs with and without cutaneous adverse food reactions

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