Identification of broad-spectrum resistance QTLs against rice blast fungus and their application in different rice genetic backgrounds

Korinsak, Siripar; Wongsaprom, Chanakarn; Jamboonsri, Watchareewan; Sriprakhon, Saengchai; Sirithunya, Kanyanat; Vanavichit, Apichart; Toojinda, Theerayut

doi:10.1007/s12041-021-01357-4

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Moreover, molecular markers have been used effectively to reduce screening and increase the efficiency of transfer of resistance in terms of pace and precision. Over a hundred blast resistance genes have been reported across all the rice chromosomes 1 , 7 . Most of these treatments are race specific and have limited durability.…”

Section: Introductionmentioning

confidence: 99%

Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis

Devanna,

Sucharita,

Sunitha

et al. 2024

Sci Rep

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Rice blast disease is the most devastating disease constraining crop productivity. Vertical resistance to blast disease is widely studied despite its instability. Clusters of genes or QTLs conferring blast resistance that offer durable horizontal resistance are important in resistance breeding. In this study, we aimed to refine the reported QTLs and identify stable meta-QTLs (MQTLs) associated with rice blast resistance. A total of 435 QTLs were used to project 71 MQTLs across all the rice chromosomes. As many as 199 putative rice blast resistance genes were identified within 53 MQTL regions. The genes included 48 characterized resistance gene analogs and related proteins, such as NBS–LRR type, LRR receptor-like kinase, NB-ARC domain, pathogenesis-related TF/ERF domain, elicitor-induced defense and proteins involved in defense signaling. MQTL regions with clusters of RGA were also identified. Fifteen highly significant MQTLs included 29 candidate genes and genes characterized for blast resistance, such as Piz, Nbs-Pi9, pi55-1, pi55-2, Pi3/Pi5-1, Pi3/Pi5-2, Pikh, Pi54, Pik/Pikm/Pikp, Pb1 and Pb2. Furthermore, the candidate genes (42) were associated with differential expression (in silico) in compatible and incompatible reactions upon disease infection. Moreover, nearly half of the genes within the MQTL regions were orthologous to those in O. sativa indica, Z. mays and A. thaliana, which confirmed their significance. The peak markers within three significant MQTLs differentiated blast-resistant and susceptible lines and serve as potential surrogates for the selection of blast-resistant lines. These MQTLs are potential candidates for durable and broad-spectrum rice blast resistance and could be utilized in blast resistance breeding.

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Section: Introductionmentioning

confidence: 99%

Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis

Devanna,

Sucharita,

Sunitha

et al. 2024

Sci Rep

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Molecular and genetic insights into secondary metabolic regulation underlying insect-pest resistance in legumes

Razzaq

Hina

Abbasi³

et al. 2023

Funct Integr Genomics

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Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease

Sahu

Sao

Choudhary³

et al. 2022

Plants

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Rice production needs to be sustained in the coming decades, as the changeable climatic conditions are becoming more conducive to disease outbreaks. The majority of rice diseases cause enormous economic damage and yield instability. Among them, rice blast caused by Magnaportheoryzae is a serious fungal disease and is considered one of the major threats to world rice production. This pathogen can infect the above-ground tissues of rice plants at any growth stage and causes complete crop failure under favorable conditions. Therefore, management of blast disease is essentially required to sustain global food production. When looking at the drawback of chemical management strategy, the development of durable, resistant varieties is one of the most sustainable, economic, and environment-friendly approaches to counter the outbreaks of rice blasts. Interestingly, several blast-resistant rice cultivars have been developed with the help of breeding and biotechnological methods. In addition, 146 R genes have been identified, and 37 among them have been molecularly characterized to date. Further, more than 500 loci have been identified for blast resistance which enhances the resources for developing blast resistance through marker-assisted selection (MAS), marker-assisted backcross breeding (MABB), and genome editing tools. Apart from these, a better understanding of rice blast pathogens, the infection process of the pathogen, and the genetics of the immune response of the host plant are very important for the effective management of the blast disease. Further, high throughput phenotyping and disease screening protocols have played significant roles in easy comprehension of the mechanism of disease spread. The present review critically emphasizes the pathogenesis, pathogenomics, screening techniques, traditional and molecular breeding approaches, and transgenic and genome editing tools to develop a broad spectrum and durable resistance against blast disease in rice. The updated and comprehensive information presented in this review would be definitely helpful for the researchers, breeders, and students in the planning and execution of a resistance breeding program in rice against this pathogen.

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Identification of broad-spectrum resistance QTLs against rice blast fungus and their application in different rice genetic backgrounds

Cited by 3 publications

References 24 publications

Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis

Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis

Molecular and genetic insights into secondary metabolic regulation underlying insect-pest resistance in legumes

Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease

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