Identification of c-Fos as a mitotic phosphoprotein: regulation of c-Fos by Aurora-A

Abstract

The c-Fos has been implicated in the regulation of gene expression under a variety of stimuli. It is known that c-Fos undergoes protein phosphorylation, which may subsequently modulate diverse functions in cells. However, less is known about the role and phosphorylation status of c-Fos during mitosis. Here, we showed that c-Fos exhibited an electrophoretic mobility up-shift as detected by SDS-PAGE during mitosis, which is an indication of protein phosphorylation. Aurora-A, but not Aurora-B or -C, serves as one of the kinases catalyzing the mitotic phosphorylation of c-Fos. The mobility up-shift was partially abolished by introducing siRNA or a catalytically inactive form of Aurora-A. Moreover, ectopic expression of the wild type, but not the catalytically inactive form of Aurora-A resulted in the alteration of c-Fos complex formation, suggesting Aurora-A is engaged in the regulation of c-Fos protein-protein interaction. These findings imply that c-Fos may undergo cell cycle dependent phosphorylation, in which some kinases including Aurora-A play a role in catalyzing the post translational modification of c-Fos.

Biology of Aurora A kinase: Implications in cancer manifestation and therapy

“…The functional significance of this phosphorylation is not yet known, but it has been predicted that its modification spanning the dimerization domain might alter the protein-protein interaction, which regulates cFos dimerization with other members. 68 So far, no changes in its stability or the transactivation ability are known to occur when Aurora A was overexpressed.…”

Cranberry Proanthocyanidin and Its Microbial Metabolite 3,4‐Dihydroxyphenylacetic Acid, but Not 3‐(4‐Hydroxyphenyl)‐Propionic Acid, Partially Reverse Pro‐Inflammatory microRNA Responses in Human Intestinal Epithelial Cells

“…Though, in inflammatory conditions, which may more closely emulate conditions like inflammatory bowel disease or cancer where the gut microbiota is in a dysbiotic state (reviewed in Ref. [58]), the microbial conversion of PAC into its metabolites was not a requisite of anti‐inflammatory activity at the miRNA level.…”

Gut Microbiota: Influence on Carcinogenesis and Modulation Strategies by Drug Delivery Systems to Improve Cancer Therapy

“…[ 65 ] Other bacterial species (e.g., Enterococcus faecalis and Bacteroides subspecies) also impair epithelial barrier integrity to facilitate pro‐tumoral bacterial invasion and biofilm formation. [ 24 ]…”

“…Studies have shown that microbiota play a crucial role in human physiological functions including nutrient absorption, [ 22 ] intestinal intrinsic neural networks regulation, [ 23 ] intestinal barrier maintenance, [ 24 ] metabolism, [ 25 ] immunity, and inflammation. [ 26 ] Dysbiosis and eubiosis have been used to describe the imbalance or balance of normal microbial communities, respectively.…”