Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device

Takakusagi, Yoichi; Takakusagi, Kaori; Kuramochi, Kouji; Kobayashi, Susumu; Sugawara, Fumio; Sakaguchi, Kengo

doi:10.1016/j.bmc.2007.09.002

Cited by 25 publications

(11 citation statements)

References 36 publications

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“…Immobilized phages have been used for the detection of β ‐galactosidase from E. coli with a detection limit of a few nanomoles (Nanduri et al , 2007), and of prostate specific membrane antigen and antibody (Yang et al ., 2006b). A T7 phage display pool was screened for affinity towards the synthetic ligand for FK506‐binding protein and irinotecan (Takakusagi et al ., 2008a), methotrexate (Takakusagi et al ., 2008b), and C10 biotinylated camptothectin (Takakusagi et al , 2007). A similar approach using phage display also led to the identification of peptides with specific binding for SiO 2 and TiO 2 (Chen et al , 2006).…”

Section: Virusesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

163

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Since the publication of the original review of piezoelectric acoustic sensors in this series there has been a consistent, gradual expansion in the number of published papers using 'quartz crystal microbalances' (QCM). Between 2001 and 2009, the number of QCM publications per annum has increased from 49 to 273, with a two-fold increase in papers per annum between 2004 and 2008. Within the field, comparing the time covered by the current to the previous review, there are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase) and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterization of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.

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Section: Virusesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

163

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“…The NHS reactive groups of DSP can easily bind covalently to primary amine groups of streptavidin. For instance, the team of Takakusagi et al [48] combined phage display technology and QCM screening to identify phages binding on a biotinylated target. The biotinylated target camptothecin (CPT), a cytotoxic molecule was fixed onto an avidin coated gold electrode on a QCM device.…”

Section: Immobilization Strategies For Small Molecules For Phage Dispmentioning

confidence: 99%

Phage display as a method for discovering cellular targets of small molecules

Dorst

Mehta

Rouah-Martin

et al. 2012

Methods

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“…Screening a library of folic acid analogues against KB carcinoma cells over-expressing folate receptors resulted in the identification of several folic acid mimetics. Additional approaches are being developed to better control screening conditions [ 147 , 148 ]. Highly sensitive biosensors, such as Biacore (based on surface plasmon resonance) and quartz crystal microbalance apparatuses (based on the piezoelectric effect), not only enable screening against minute amounts of target but, more importantly, also allow the monitoring of phage-target interactions in real time.…”

Section: Future Prospectsmentioning

confidence: 99%

Peptide Phage Display as a Tool for Drug Discovery: Targeting Membrane Receptors

2011

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Ligands selected from phage-displayed random peptide libraries tend to be directed to biologically relevant sites on the surface of the target protein. Consequently, peptides derived from library screenings often modulate the target protein's activity in vitro and in vivo and can be used as lead compounds in drug design and as alternatives to antibodies for target validation in both genomics and drug discovery. This review discusses the use of phage display to identify membrane receptor modulators with agonistic or antagonistic activities. Because isolating or producing recombinant membrane proteins for use as target molecules in library screening is often impossible, innovative selection strategies such as panning against whole cells or tissues, recombinant receptor ectodomains, or neutralizing antibodies to endogenous binding partners were devised. Prominent examples from a two-decade history of peptide phage display will be presented, focusing on the design of affinity selection experiments, methods for improving the initial hits, and applications of the identified peptides.

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Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device

Cited by 25 publications

References 36 publications

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Phage display as a method for discovering cellular targets of small molecules

Peptide Phage Display as a Tool for Drug Discovery: Targeting Membrane Receptors

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