Identification of Cadmium-responsive Kandelia obovata SOD family genes and response to Cd toxicity

Pan, Chenglang; Lu, Haoliang; Yu, Jinfeng; Liu, Jingchun; Liu, Yumei; Yan, Chongling

doi:10.1016/j.envexpbot.2019.02.018

Cited by 29 publications

(21 citation statements)

References 54 publications

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“…The wheat roots had higher SOD activity under Cd stress and this result was due to intensities of Mn-SOD and Cu/Zn-SOD1-3. The findings of the current experiment were in line with the results of Pan et al [15]. Also, this induction in SOD activity was maintained by GLA application.…”

Section: The Activities Of Sod and Noxsupporting

confidence: 93%

Does Exogenously Applied Gallic Acid Regulate the Enzymatic and Non-Enzymatic Antioxidants in Wheat Roots Exposed to Cadmium Stress?

Konakci

2019

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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The aim of the current study was to determine whether gallic acid (GLA) triggers the growth, osmoregulation and antioxidant system related to defense mechanisms in wheat roots to cadmium (Cd)induced oxidative stress. For this purpose, wheat plants were hydroponically grown for 21 (d) and were treated with GLA (GLA1-2; 25 and 75 M), Cd stress (Cd1-2; 100 and 200 M) and their combination for 7 d. The significant reduction in growth (RGR) and osmotic potential () was observed under stress. After GLA applications in response to stress, RGR,  and proline (Pro) increased, except for 200 M Cd plus 75 M GLA. Under stress, hydrogen peroxide (H2O2) was induced by the activated superoxide dismutase (SOD) activity but, NADPH oxidase (NOX) had no contribution on the accumulation of H2O2. Despite of the increased catalase (CAT) and glutathione reductase (GR), H2O2 did not eliminate and then lipid peroxidation (TBARS content) was induced with the decreased scavenging capacity of hydroxyl radical (OH ) under stress. Besides, to remove of H2O2 content produced by SOD, H2O2 could effectively scavenge through CAT activity in combination form of GLA and Cd. On the other hand, GLA did not induce the enzymes and non-enzymes related to Asada-Halliwell cycle (ascorbate peroxidase (APX), GR, dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), reduced and oxidized contents of glutathione (GSH and GSSG contents). Under high Cd concentration, GLA2 could not eliminate H2O2 content because of increased NOX activity and then in this group (Cd2+GLA2) the scavenging capacity of OH  did not change and TBARS content increased.

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Section: The Activities Of Sod and Noxsupporting

confidence: 93%

Does Exogenously Applied Gallic Acid Regulate the Enzymatic and Non-Enzymatic Antioxidants in Wheat Roots Exposed to Cadmium Stress?

Konakci

2019

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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“…Sunflower seeds under Cd stress showed reduced biomass, carotenoid, and low chlorophyll concentration with an increase Cd in the shoot and root. Pan et al [186] examined the genetic insight of high Cd-tolerant Kandelia obovata, a mangrove plant, and reported two genes KoFSD2 and KoCSD3 that showed greater SOD levels and differentially maintained the reactive oxygen mechanism when overexpressed in Nicotiana benthamiana under Cd toxicity.…”

Section: Antioxidant Defense: a Key Mechanism Of Cadmium Tolerance Anmentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

185

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Cadmium (Cd) is one of the most toxic metals in the environment, and has noxious effects on plant growth and production. Cd-accumulating plants showed reduced growth and productivity. Therefore, remediation of this non-essential and toxic pollutant is a prerequisite. Plant-based phytoremediation methodology is considered as one a secure, environmentally friendly, and cost-effective approach for toxic metal remediation. Phytoremediating plants transport and accumulate Cd inside their roots, shoots, leaves, and vacuoles. Phytoremediation of Cd-contaminated sites through hyperaccumulator plants proves a ground-breaking and profitable choice to combat the contaminants. Moreover, the efficiency of Cd phytoremediation and Cd bioavailability can be improved by using plant growth-promoting bacteria (PGPB). Emerging modern molecular technologies have augmented our insight into the metabolic processes involved in Cd tolerance in regular cultivated crops and hyperaccumulator plants. Plants’ development via genetic engineering tools, like enhanced metal uptake, metal transport, Cd accumulation, and the overall Cd tolerance, unlocks new directions for phytoremediation. In this review, we outline the physiological, biochemical, and molecular mechanisms involved in Cd phytoremediation. Further, a focus on the potential of omics and genetic engineering strategies has been documented for the efficient remediation of a Cd-contaminated environment.

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“…The Cu/ZnSODs, Fe-and MnSODs bind to Cu/Zn, Fe, and Mn metal cofactors depending on the cellular localization and the availability of metal ions, respectively (Tyagi et al, 2017). In addition, under conditions of Mn or Cu restriction, the activity of MnSOD or Cu/ZnSOD are significantly inhibited, which suggested that the content of each metal ion in the subgroup of SOD proteins may determine the equilibrium between these isozymes (del Río et al, 1991) Gene expression analyses of SOD family genes have demonstrated that SODs have diverse expression patterns in different tissues of plants (Lin & Lai, 2013;Feng et al, 2016;Pan et al, 2019). In this study, analysis of tissue-specific expression of ZmSODs was performed using RNA-seq data (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For the three main types of SODs identified in plants, Cu/ZnSOD is the most abundant, and is mainly distributed in the chloroplasts, plants cytoplasm, peroxisomes, and/or the extracellular space (Song et al, 2018). FeSOD is considered the oldest type of SOD, and is mainly localized in the chloroplast with a few proteins found in the cytoplasm, whereas MnSOD typically has a mitochondrial localization (Pan et al, 2019;Perry et al, 2010). With the development of second-generation sequencing technologies and increased elucidation of the multiple functions of SOD genes, SOD gene families have been identified at the genome-wide level in various plants (Wang et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the superoxide dismutase (SOD) gene family in Zostera marina and expression profile analysis under temperature stress

Zang

Shang

et al. 2020

PeerJ

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Superoxide dismutases (SODs) serve as the first line of defense in the plant antioxidant enzyme system, and play a primary role in the removal of reactive oxygen species (ROS). However, our understanding of the functions of the SOD family in Zostera marina is limited. In this study, a systematic analysis was conducted on the characteristics of the SOD genes in Z. marina at the whole-genome level. Five SOD genes were identified, consisting of two Cu/ZnSODs, two FeSODs, and one MnSOD. Phylogenetic analysis showed that ZmSOD proteins could be divided into two major categories (Cu/ZnSODs and Fe-MnSODs). Sequence motifs, gene structure, and the 3D-modeled protein structures further supported the phylogenetic analysis, with each subgroup having similar motifs, exon-intron structures, and protein structures. Additionally, several cis-elements were identified that may respond to biotic and abiotic stresses. Transcriptome analysis revealed expression diversity of ZmSODs in various tissues. Moreover, qRT-PCR analysis showed that the expression level of most ZmSOD genes trended to decreased expression with the increase of temperature, indicating that heat stress inhibits expression of ZmSODs and may result in reduced ability of ZmSODs to scavenge ROS. Our results provide a basis for further functional research on the SOD gene family in Z. marina, which will help to determine the molecular mechanism of ZmSOD genes in response to environmental stress.

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Identification of Cadmium-responsive Kandelia obovata SOD family genes and response to Cd toxicity

Cited by 29 publications

References 54 publications

Does Exogenously Applied Gallic Acid Regulate the Enzymatic and Non-Enzymatic Antioxidants in Wheat Roots Exposed to Cadmium Stress?

Does Exogenously Applied Gallic Acid Regulate the Enzymatic and Non-Enzymatic Antioxidants in Wheat Roots Exposed to Cadmium Stress?

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Genome-wide analysis of the superoxide dismutase (SOD) gene family in Zostera marina and expression profile analysis under temperature stress

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