Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis

Abstract

Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams, and one human patient yielded the same BRENDA type when sampled 6 weeks later. The 14 human isolates examined produced 11 distinct BRENDA types. Forty-three C. coli isolates from pigs were represented by 20 BRENDA types. Ten C. coli isolates from the feces of gulls yielded five different BRENDA types. Thirty-two C. coli isolates from live chickens and processed chicken yielded five different BRENDA types. Three human isolates had identical DNA patterns; two were from brothers living in the same house, and the third was from a human with no apparent relationship to the brothers. Another human isolate was identical to a poultry isolate. None of the pig strains had DNA patterns resembling those of human strains, nor were the DNA patterns like those of any strains recovered from poultry or gulls. Four C. coli isolates were subcultured onto agar 23 times over a period of 45 days, and their BRENDA patterns were preserved. BRENDA shows great promise for use in epidemiological studies of C. coli. Campvlobacter (oli was first described as Vibrio coli (7) and was thought to be the etiological agent of swine dysentry (6). Veron and Chatelain (36) classified the genus Cainpylobacteer and renamed V. coli as C. coli. This renaming has recently been accepted by the International Committee on Systematic Bacteriology (27). The feature which distinguishes C. coli from C. jejiuni is its inability to hydrolyze hippurate in the test described by Harvey (9) and Skirrow and Benjamin (30). Although C. (oli is less frequently isolated from cases of human diarrhea than is C. jejiunii (3, 4. 11, 14, 22, 31), there appears to be no obvious difference in disease severity for humans with either of these two species (29). Each year in New Zealand a number of acute enteritis cases in humans are found to be caused by C. (oli (D. M. Norris, personal communication), but their source is un-* Corresponding author.

Effective Recovery of Bacterial DNA and Percent-Guanine-Plus-Cytosine-Based Analysis of Community Structure in the Gastrointestinal Tract of Broiler Chickens

“…Molecular techniques have been employed to monitor for the presence of specific bacterial pathogens in chicken digesta samples (2,6,16,26). In order to place findings from such single-species studies in perspective, some knowledge of the total bacterial community composition of this system must be obtained.…”

DNA fingerprinting ofStreptococcus uberis: a useful tool for epidemiology of bovine mastitis

“…1 as a typing method. Many reports have shown the value of endonuclease digestion of bacterial DNA for typing isolates (2)(3)(4)(5)(6)(12)(13)(14). However, the technique was used for fine discrimination or comparison of groupings obtained by other typing methods.…”

“…This is even more significant when one considers that in a recent finding using a typing system based on lytic phage it was concluded that the differences between the typable bacteria depended on very weak and variable reactions, (Hill, unpublished observations A and B, three and four strains respectively from the same mammary, gland of two cows5 on separate occasions during recurrent infections. [1][2][3][4] represent tracks scanned with an image analyser and plotted in Fig. 3; 5, DNA from an organism which was shown biochemically to be S. bovis; 6, DNA from an organism which was shown biochemically to be S. faeciurn; 7, DNA from S. uberis showing fragment of Irge plasmid at 7-5 Kb.…”

Investigation of zoonotically acquired Campylobacter jejuni enteritis with serotyping and restriction endonuclease DNA analysis

“…Recently, serotyping and chromosomal DNA restriction enzyme analysis have been used successfully to investigate water-or milk-borne C. jejuni and C. coli outbreaks (4,8,13,19). Strains of the same genotype would be expected to have the same restriction patterns, whereas strains of different genotypes could possess genes encoding the biosynthesis of the same thermostable antigenic specificity.…”