Identification of cancer stem cells in vincristine preconditioned SGC7901 gastric cancer cell line

Xue, Zhe; Yan, Hui; Li, Juntang; Liang, Shuli; Cai, Xiqiang; Xiong, Chen; Wu, Qiong; Gao, Liucun; Wu, Kaichun; Nie, Yongzhan; Fan, Daiming

doi:10.1002/jcb.23356

Cited by 71 publications

(70 citation statements)

References 54 publications

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“…It was previously reported that synchronous ovarian metastasis was an unfavorable factor correlated with poor survival (14), which was consistent with our study, suggesting that the metastasis-free interval was shortened between primary tumor diagnosis and ovarian metastasis.…”

Section: Univariate Analysis Multivariate Analysis ------------------supporting

confidence: 93%

Clinical characteristics and prognostic analysis of Krukenberg tumor

Zhao

et al. 2015

Molecular and Clinical Oncology

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Abstract. Krukenberg tumor is a rare metastastic tumor of the ovary, characterized by poor prognosis. In order to analyze the clinical characteristics and prognostic factors, we retrospectively investigated 128 patients who were diagnosed with Krukenberg tumor between January, 1990 and December, 2010. The median patient age was 48 years. The median overall survival (OS) of Krukenberg tumor for all patients was 16 months (95% CI: 15-19 months). The median OS among patients with Krukenberg tumors of gastric, colorectal, breast and other origins (including appendix, gallbladder, small intestine and unknown primary) was 11, 21.5, 31 and 19.5 months, respectively (P<0.0001). In the univariate analysis, synchronous metastasis, no chemotherapy, ovarian metastasis beyond the pelvis, ascites and no metastasectomy were identified as significant poor prognostic factors. The multivariate analysis suggested that synchronous metastasis (P=0.0080), pelvic invasion (P=0.0138), ascites (P<0.0001) and no metastasectomy (P=0.0060) were independent factors for predicting unfavorable OS. It was suggested that the prognosis of Krukenberg tumor is dismal and ovarian metastasectomy may prove beneficial. Adequate treatment planning is required for this group of patients. IntroductionKrukenberg tumor is a rare metastatic signet ring cell tumor of the ovary, accounting for 1-2% of all ovarian tumors. The stomach is the primary site in the majority of Krukenberg tumor cases, followed by carcinomas of the colon, appendix and breast, particularly invasive lobular carcinoma (1). The eponym was attributed to this tumor following the description of 5 cases by Friedrich Krukenberg (1871-1946) in 1896, who described it as being common among young women, presenting with ascites, an uneven knobby ovarian surface and lymphatic involvement (2). These tumors are characterised by uncertain pathogenesis, challenging etiological diagnosis and poorer prognosis compared with their primaries. Previously, any metastatic ovarian cancer was referred to as Krukenberg tumor; however, Novak and Gray (3) created new diagnostic criteria to eliminate any confusion. Accordingly, a mucin-secreting signet ring cell carcinoma in the dense fibroblastic stroma of the ovary is referred to as Krukenberg tumor. The diagnosis of Krukenberg tumor is currently based on the diagnostic criteria of the World Health Organization based on the pathological description by Serov and Scully (4). The presence of the following characteristics is required for diagnosis: Stromal involvement, mucin-producing neoplastic signet ring cells and ovarian stromal sarcomatoid proliferation.Krukenberg tumor is considered as a late-stage disease with poor prognosis and may account for 30-40% of metastatic cancers to the ovaries (5). The treatment approach to these metastatic ovarian tumors remains controversial. To date, treatment mainly consists of ovarian metastasectomy, chemotherapy or radiotherapy; however, the optimal treatment has not yet been established. As the biological behavior and clinical...

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Section: Univariate Analysis Multivariate Analysis ------------------supporting

confidence: 93%

Clinical characteristics and prognostic analysis of Krukenberg tumor

Zhao

et al. 2015

Molecular and Clinical Oncology

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“…Briefly, Transwell units with 8.0-µm-pore polycarbonate filters (Corning Incorporated, Corning, NY, USA) were precoated with 50 µl of 1:5 diluted Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). A total volume of 200 µl of gastric cancer cell suspension, which contained ~1x10 5 rapamycin-treated cells, was added to the upper compartment of the precoated units. The units were then transferred to the wells of the culture plate and incubated for 24 h at 37˚C.…”

Section: Methodsmentioning

confidence: 99%

“…In numerous types of cancer, including gastric cancer, CD44 has been associated with increased invasion, metastasis and poor prognosis (3,4). This molecule has also been identified as a marker of stem-like gastric cancer cells (5,6); however, its role in this phenotype remains to be defined.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells

Liang

et al. 2016

Oncology Letters

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Abstract. Hypoxia induces proliferation and invasion in cancer cells via hypoxia-inducible factor (HIF)-1α. The cell adhesion molecule cluster of differentiation (CD) 44 has been associated with increased cell invasion and metastasis. Whether hypoxia regulates the expression of CD44 in gastric cancer cells remains to be established. In the current study, the effects of hypoxia on HIF-1α and CD44 expression levels in human gastric cell lines SGC-7901 and BGC-823 were evaluated. The cells were cultured in 1% O 2 for 1 week and then treated with 20 nM rapamycin for 72 h. Cell viability was evaluated using the Cell Counting kit-8 assay, and cell invasion was detected by the Transwell invasion assay. The protein and messenger (m) RNA expression levels of HIF-1α and CD44 were detected using western blotting and reverse transcription-quantitative polymerase chain reaction, respectively. The results revealed that cell viability and invasion increased under hypoxic conditions, but decreased following rapamycin treatment in SGC-7901 and BGC-823 cells. Hypoxia also increased the protein and mRNA expression levels of HIF-1α and CD44 in these two cell lines. However, this hypoxia-induced increase in HIF-1α and CD44 protein and mRNA expression levels was inhibited by rapamycin. These findings suggest that hypoxia induced the proliferation and invasion of SGC-7901 and BGC-823 cells. Furthermore, CD44 expression levels were potentially associated with HIF-1α expression levels. Therefore, in gastric cancer cells, hypoxia may regulate CD44 expression via HIF-1α in order to promote cell proliferation and invasion.

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“…The PKH-26 hi cells are highly enriched in CSCs (*1:1), and represent the only cell subset capable of reconstituting a mammary tumor; thus, suggesting that breast CSCs undergo a limited number of divisions. Other researchers have also used this strategy to isolate CSCs from different types of human tumors, such as gastrointestinal cancers, glioblastomas, and nasopharyngeal carcinomas [160][161][162][163].…”

Section: Cancer Cell Divisionmentioning

confidence: 99%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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Cancer stem cells (CSCs) constitute a subpopulation of cancer cells that have the potential for self-renewal, multipotent differentiation, and tumorigenicity. Studies on CSC biology and CSC-targeted therapies depend on CSC isolation and/or enrichment methodologies. Scientists have conducted extensive research in this field since John Dick's group successfully isolated CSCs based on the expression of the CD34 and CD38 surface markers. Progress in CSC research has been greatly facilitated by the enrichment and isolation of these cells. In this review, we summarize the current strategies used in our and other laboratories for CSC isolation and enrichment, including methods based on stem cell surface markers, intracellular enzyme activity, the concentration of reactive oxygen species, the mitochondrial membrane potential, promoter-driven fluorescent protein expression, autofluorescence, suspension/adherent culture, cell division, the identification of side population cells, resistance to cytotoxic compounds or hypoxia, invasiveness/adhesion, immunoselection, and physical property. Although many challenges remain to be overcome, it is reasonable to believe that more reliable, efficient, and convenient methods will be developed in the near future.

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Identification of cancer stem cells in vincristine preconditioned SGC7901 gastric cancer cell line

Cited by 71 publications

References 54 publications

Clinical characteristics and prognostic analysis of Krukenberg tumor

Clinical characteristics and prognostic analysis of Krukenberg tumor

Hypoxia regulates CD44 expression via hypoxia-inducible factor-1α in human gastric cancer cells

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

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