Identification of Candida species using CHROM agar.

Khadka, S; Regmi, Pravesh; Giri, Sandip Kumar; Shah, Pradeep Kumar; Mishra, Shyam Kumar

doi:10.55530/ijmbiosnepal.v1i4.15

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…This medium was used as a differential medium with accurate results in the diagnosis of Candida species. This finding is consistent with what was stated by Jain, et al [27] , It also came in accordance with the study of Khadka, et al [28] regarding the isolation and quick identification of Candida species, as one of the most essential media utilized in the field of fungal diagnostics, since the diagnosis is dependent on the medium's coloring.…”

Section: Growth On Hicrome Candida Differential Agarsupporting

confidence: 92%

Candida spp. Associated With COVID-19 And Its Susceptibility To Some Antifungals

Alkhuzaie

Jasim²

2022

Al-Qadisiyah Journal of Pure Science

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This study aimed to conduct a survey of the Candida species associated with COVID-19 viral infection in 150 patients who were admitted to the intensive care unit (ICU) in Al-Diwaniyah Teaching Hospital in Al-Diwaniyah City, Iraq, for a five-month period from October 2021 to February 2022. The results indicated the dominance of Candida spp. over the rest of the isolated fungal species, with 97 isolates (64.66%). C. albicans was shown to be the most abundant species with a percentage of 55.67 percent (P 0.05), compared to the other Candida species that were isolated (Candida tropicalis 13.4 percent, Candida glabrata 12 percent, Candida krusie and Candida parapsilosis with 9.28 percent). In addition, we brought attention to the excellent action of the antifungals amphotericin B, itraconazole, and voriconazole, all of which have a high susceptibility rate.

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Section: Growth On Hicrome Candida Differential Agarsupporting

confidence: 92%

Candida spp. Associated With COVID-19 And Its Susceptibility To Some Antifungals

Alkhuzaie

Jasim²

2022

Al-Qadisiyah Journal of Pure Science

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“…(29%), Candida Glabrata (11%), and Candida Tropicalis (8%). Khadka S et al [ 22 ] reported the most predominant species as Candida albicans 64.7%¸ followed by other Candida species (15/6%), Candida tropicalis 11.7%¸ and Candida glabrata 7.8%.…”

Section: Discussionmentioning

confidence: 99%

“…Candida albicans produces colonies with varying shades of green, Candida tropicalis produces colonies with a blue-gray color, and Candida glabrata produces pink colonies. The other colors produced by Candida , such as purple and yellow, are classified under the group of other types of Candida [ 22 ]. The relationship between blood glucose indices (FBS, HbA1c), IL-10, and IFN-γ and the presence or absence of candidiasis were also evaluated in the study groups.…”

Section: Methodsmentioning

confidence: 99%

The relation between serum levels of interleukin 10 and interferon-gamma with oral candidiasis in type 2 diabetes mellitus patients

et al. 2022

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Background Type 2 Diabetes mellitus (T2DM) is one of the most common endocrine diseases that weakens the immune system. Candida albicans, is part of the natural oral flora and increases in cases of compromised immune systems. The exact cause of the increased prevalence of candidiasis in patients with T2DM is still unclear. The study aimed to correlate serum interleukin 10 (IL-10) and interferon-gamma cytokines (IFN-γ) with oral candidiasis in T2DM. Methods In this case–control study, 81 patients with T2DM and 41 non-diabetic individuals aged 30 to 70 years participated. Demographic information, a Blood sample (for blood glucose and cytokine tests), and an oral cotton swab sample from each individual were obtained. The samples were then incubated in a Sabroud dextrose agar medium. Colony growth was calculated and the type of yeast species in individuals with oral candidiasis was identified by culture in CHROMagar Candida medium. IL-10 and IFN-γ were measured by ELISA kit and the data were analyzed using SPSS-18. Results An overall of 122 participants comprised 73.77% females and 26.22% males. An increase in interleukin-10 by 40% and a decrease in IFN-γ by 6% can increase oral candidiasis prevalence among diabetic patients. Candida albicans was the most prevalent Candida species (spp.) in the diabetic and non-diabetic groups. The presence of oral candidiasis was not associated with HbA1c or FBS levels in both groups. Conclusion In the diabetic population, an increase in IL-10 or a decrease in IFN-γ may be associated with an increased risk of oral candidiasis.

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“…The germ tube formation test was tested by culturing a single candidal colony with human serum at 37℃ for 2 h, as primary identification for C.albicans and C. dubliniensis. A presumptive identification based on the characteristic colony colors of candida colonies was done by subculturing aerobically at 37℃ for 24 h on CHROMagar Candida (75006 Paris, France) [19,20]. For more precision pure colonies were identified up to species based on their carbohydrate assimilation pattern by the VITEK 2 compact (biomerieux, France) yeast identification system [21,22].…”

Section: Sampling and Culturingmentioning

confidence: 99%

Association between Candida species and caries index in children

Najeeb¹,

Muhammad²,

Mustafa³

2023

djm

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Background: candida species is one of the most important opportunistic oral fungal flora that contributed to oral candidal infections. Recently some studies indicate the association between increased caries incidence in children with oral candidal carriage. Objective: To candida species is one of the most important opportunistic oral fungal flora that contributed to oral candidal infections. Recently some studies indicate the association between increased caries incidence in children with oral candidal carriage. Patients and Methods: Sixty-one children with ages 6 to 12 years , DMFT, dmft, and OHI-S were measured and oral swabs were tested on three different surfaces (lingual, occlusal, and buccal) then cultured on SDA as a primary medium, and selective medium on CHROMagar, also germ tube test was done and for more precision, pure colonies were identified up to species based on their carbohydrate assimilation pattern by the VITEK 2 compact Results: 22 (36.1%) children with candida carriage, C.albicans 12, (54%), C.dubliniensis 4,( 18.18%), C.krusei 4, (18.18%), C. tropicalis 2, ( 9.1%). There was no association between candidal species in the oral cavity and caries index (DMFT, dmft). Conclusion: Non-significant weak negative correlation appeared between candidal species in the oral cavity and caries index in children. The most predominant candidal species was C.albicans.

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Identification of Candida species using CHROM agar.

Cited by 3 publications

References 15 publications

Candida spp. Associated With COVID-19 And Its Susceptibility To Some Antifungals

Candida spp. Associated With COVID-19 And Its Susceptibility To Some Antifungals

The relation between serum levels of interleukin 10 and interferon-gamma with oral candidiasis in type 2 diabetes mellitus patients

Association between Candida species and caries index in children

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