DOI: 10.14264/uql.2019.886
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Identification of candidate genes for blackleg resistance in the new Brassica juncea canola

Abstract: Brassica species are at the high risk of severe crop loss due to pathogens, especially Leptosphaeria maculans (the causal agent of blackleg). To date, only two blackleg resistance genes, Rlm2 and LepR3, have been cloned, both from Brassica napus, which are allelic variants encoded by the LepR3/Rlm2 locus on chromosome A10. LepR3 confers resistance to avirulence gene AvrLm1, which is also recognised by Rlm1; while Rlm2 interacts with AvrLm2. In this study, seven LepR3/Rlm2 allelic variants have been isolated fr… Show more

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Cited by 3 publications
(3 citation statements)
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“…Leaf samples from subsequent generations were collected in tubes and DNA was extracted using the 2 × CTAB method (Wang, 2007). For detection of the ALS mutations defined in For amplicon sequencing, PCR was completed using the VBO19 primer pair to amplify all three camelina ALS gene copies.…”
Section: Methodsmentioning
confidence: 99%
“…Leaf samples from subsequent generations were collected in tubes and DNA was extracted using the 2 × CTAB method (Wang, 2007). For detection of the ALS mutations defined in For amplicon sequencing, PCR was completed using the VBO19 primer pair to amplify all three camelina ALS gene copies.…”
Section: Methodsmentioning
confidence: 99%
“…In addition, as with the case of Rlm1 and LepR3, it is unclear if Rlm5 and Rlm9 are allelic variants or independent genes (Ghanbarnia et al 2018). Rlm6, which has been genetically mapped onto A07 and B04 chromosomes of B. juncea (Yang 2018) and interacts with AvrLm6, is now utilized in canola cultivars. Due to overlap in the resistance genes mapped by different groups and falling into the same linkage group, Rlm6 is likely to be the same gene as Jlm1 (Brun et al 2010).…”
Section: Major Resistance Genesmentioning
confidence: 99%
“…Earlier, this gene was reported to have two alleles, LepR4a and LepR4b , each having different levels of resistance [ 86 ]. In B. juncea , another gene Rlm6 has also been genetically mapped onto two different chromosomes, A07 and B04 [ 91 ]. Both LepR4 and Rlm6 are yet to be cloned.…”
Section: The Current Resistance Genes Go Beyond Simple Allelismmentioning
confidence: 99%