Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.

Szeliga, Magdalena; Ciura, Joanna; Grzesik, Michalina; Tyrka, Mirosław

doi:10.1016/j.gene.2019.143962

Cited by 7 publications

(1 citation statement)

References 60 publications

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“…Metabolite extracts were generated in the same manner as described above for daffodils (80% methanol extraction). On a separate Veratrum nigrum plant, RNA extraction and cDNA synthesis was performed on leaf base, mid and tip sections (similarly to daffodils, see methods, RNA extraction) and RT-PCR assays were performed to quantify expression of the known verazine pathway gene GABAT1 using Actin as an internal reference, based on the Actin target sequence previously reported (Supplementary Table 4) 109 .…”

Section: Stable Isotope Precursor Feeding Experiments To Track Active...mentioning

confidence: 99%

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

Mehta¹,

Meng²,

Zare³

et al. 2023

Preprint

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Numerous eukaryotic toxins that accumulate in geophytic plants are valuable in the clinic, yet their biosynthetic pathways have remained elusive. A lead example is the >150 Amaryllidaceae alkaloids (AmAs) including galantamine, an FDA-approved treatment for Alzheimer's disease. We show that while AmAs accumulate to high levels in many tissues in daffodils, biosynthesis is localized to nascent, growing tissue at the base of leaves. A similar trend is found for the production of steroidal alkaloids (e.g. cyclopamine) in corn lily. This model of active biosynthesis enabled elucidation of a complete set of biosynthetic genes for the production of AmAs. Taken together, our work sheds light on the developmental and enzymatic logic of diverse alkaloid biosynthesis in daffodil. More broadly, it suggests a paradigm for biosynthesis regulation in monocot geophytes where plants are protected from herbivory through active charging of newly formed cells with eukaryotic toxins that persist as aboveground tissue develops.

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Section: Stable Isotope Precursor Feeding Experiments To Track Active...mentioning

confidence: 99%

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

Mehta¹,

Meng²,

Zare³

et al. 2023

Preprint

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show abstract

A multi-evaluating strategy for raw and processed Veratrum nigrum L.: Fingerprinting combined with quantitative analysis based on multivariate chemometric methods

Yao

Zhang

et al. 2023

Arabian Journal of Chemistry

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A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

Mehta,

Meng,

Zare

et al. 2024

Cell

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Identification of candidate genes involved in steroidal alkaloids biosynthesis in organ-specific transcriptomes of Veratrum nigrum L.

Cited by 7 publications

References 60 publications

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

A multi-evaluating strategy for raw and processed Veratrum nigrum L.: Fingerprinting combined with quantitative analysis based on multivariate chemometric methods

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes

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