Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system

Ma, Rongrong; Sun, Jing; Fang, Wenhong; Dong, Yanjun; Ruan, Jiming; Yang, Xianle; Hu, Kun

doi:10.1007/s10695-018-0554-5

Cited by 1 publication

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The yeast two-hybrid system enables the in vivo detection of interacting proteins in order to reveal the biological roles of a known protein (12). Following a series of optimizations and development by Fields and Song (13), the yeast two-hybrid system was considered to bea classical method ofidentifying and studying protein-protein interactions.…”

Section: Identification Of Hela Cell Proteins That Interact Withmentioning

confidence: 99%

Identification of HeLa cell proteins that interact with Chlamydia�trachomatis glycogen synthase using yeast two‑hybrid assays

Sun

et al. 2020

Mol Med Report

View full text Add to dashboard Cite

Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (Glga) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. in order to improve understanding of the roles of Glga in chlamydial pathogenesis, four proteins that interact with Glga, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin-like actin-capping protein and apolipoprotein a-i binding protein were identified using yeast two-hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of Glga in C. trachomatis infection.

show abstract

Section: Identification Of Hela Cell Proteins That Interact Withmentioning

confidence: 99%

Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system

Cited by 1 publication

References 47 publications

Identification of HeLa cell proteins that interact with Chlamydia�trachomatis glycogen synthase using yeast two‑hybrid assays

Identification of HeLa cell proteins that interact with Chlamydia�trachomatis glycogen synthase using yeast two‑hybrid assays

Contact Info

Product

Resources

About