Identification of cardiorespiratory toxic components of Nemopilema nomurai jellyfish venom using sequential chromatography methods

Prakash, Ramachandran Loganathan Mohan; Hwang, Du Hyeon; Asirvatham, Ravi Deva; Hong, Il-Hwa; Kang, Changkeun; Kim, Euikyung

doi:10.1016/j.toxicon.2023.107126

Toxicon

2023

DOI: 10.1016/j.toxicon.2023.107126

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Identification of cardiorespiratory toxic components of Nemopilema nomurai jellyfish venom using sequential chromatography methods

Ramachandran Loganathan Mohan Prakash

Du Hyeon Hwang

Ravi Deva Asirvatham

et al.

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2024

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Cited by 4 publications

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Jellyfish stings‐induced cardiac failure was ameliorated through AAG‐mediated glycogen‐driven ATP production

Qin,

Chen,

Liu

et al. 2024

Exploration

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Jellyfish stings have become a common injury among fishermen and divers. Severe jellyfish stings could worsen cardiac function and even cause cardiac complications, ultimately leading to cardiac failure (CF). Currently, there are no effective drugs available. Single cell sequencing revealed alpha‐1 acid glycoprotein (AAG), an energy regulatory protein targeting to glycogen, was highly expressed in jellyfish stings‐induced CF patients. However, the mechanism remains elusive. It is postulated that AAG could increase glycogen metabolism, protecting against jellyfish stings‐induced CF. AAG deletion exacerbated CF, while exogenous and endogenous AAG ameliorated CF. AAG also rescued the decline triggered by the AAG knockout (KO). Intriguingly, AAG improved cardiac function and metabolic adaptation by glycogen‐driven ATP production, shifting mitochondrial/glycolytic ATP production towards glycolysis. Sorted by single‐cell RNA sequencing and spatial transcription technology, CC‐chemokine receptor 5 (CCR5) and Peroxisome proliferator‐activated receptor‐gamma coactivator‐1alpha (PGC‐1α) were differentially expressed. Mechanistically, CCR5 inhibitor MVC abolished AAG's protective effect and PGC‐1α overexpression. Collectively, jellyfish stings‐induced CF was ameliorated through AAG‐mediated glycogen‐driven ATP production, promoting glycolytic/mitochondrial metabolic switches to rely energetically primarily on glycolysis, which might serve as a therapeutic target of CF.

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Jellyfish stings‐induced cardiac failure was ameliorated through AAG‐mediated glycogen‐driven ATP production

Qin,

Chen,

Liu

et al. 2024

Exploration

View full text Add to dashboard Cite

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A new invertebrate NPY-like polypeptide, ZoaNPY, from the Zoanthus sociatus, as a novel ligand of human NPY Y2 receptor rescues vascular insufficiency via PLC/PKC and Src- FAK-dependent signaling pathways

Chen,

Xu,

Zhao

et al. 2024

Pharmacological Research

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Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

Geng,

Wang,

Hou

et al. 2024

Marine Drugs

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The giant jellyfish Nemopilema nomurai sting can cause local and systemic reactions; however, comparative analysis of the tentacle extract (TE) and nematocyst venom extract (NV), and its toxicity, mechanism, and potential intervention are still limited. This study compared venom from TE and NV for their composition, toxicity, and efficacy in vitro and in vivo used RAW264.7 cells and ICR mice. A total of 239 and 225 toxin proteins were identified in TE and NV by proteomics, respectively. Pathological analysis revealed that TE and NV caused heart and liver damage through apoptosis, necrosis, and inflammation, while TE exhibited higher toxicity ex vivo and in vivo. Biochemical markers indicated TE and NV elevated creatine kinase, lactatedehydrogenase, and aspartate aminotransferase, with the TE group showing a more significant increase. Transcriptomics and Western blotting indicated both venoms increased cytokines expression and MAPK signaling pathways. Additionally, 1 mg/kg PACOCF3 (the phospholipase A2 inhibitor) improved survival from 16.7% to 75% in mice. Our results indicate that different extraction methods impact venom activities, tentacle autolysis preserves toxin proteins and their toxicity, and PACOCF3 is a potential antidote, which establishes a good extraction method of jellyfish venom, expands our understanding of jellyfish toxicity, mechanism, and provides a promising intervention.

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Identification of cardiorespiratory toxic components of Nemopilema nomurai jellyfish venom using sequential chromatography methods

Cited by 4 publications

References 51 publications

Jellyfish stings‐induced cardiac failure was ameliorated through AAG‐mediated glycogen‐driven ATP production

Jellyfish stings‐induced cardiac failure was ameliorated through AAG‐mediated glycogen‐driven ATP production

A new invertebrate NPY-like polypeptide, ZoaNPY, from the Zoanthus sociatus, as a novel ligand of human NPY Y2 receptor rescues vascular insufficiency via PLC/PKC and Src- FAK-dependent signaling pathways

Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai

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