Identification of Caveolae-like Structures on the Surface of Intact Cells Using Scanning Force Microscopy

Lucius, Hans; Friedrichson, Tim; Kurzchalia, Teymuras V.; Lewin, Gary R.

doi:10.1007/s00232-003-2029-5

Cited by 13 publications

(14 citation statements)

References 30 publications

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“…In this work we obtain the domain sizes from the walking diffusion model and find 130 nm for the slow fraction and 250 nm for the fast fraction. These values are in agreement with scanning force microscopy experiments on living CHO cells by Lucius et al (209). They find two populations of pits with mean diameters of around 100 nm and 200 nm.…”

Section: Discussionsupporting

confidence: 92%

Membrane heterogeneity – from lipid domains to curvature effects

Semrau¹,

Schmidt²

2009

Soft Matter

102

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Section: Discussionsupporting

confidence: 92%

Membrane heterogeneity – from lipid domains to curvature effects

Semrau¹,

Schmidt²

2009

Soft Matter

102

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“…Lucius et al (2003) show a distribution of diameters from 60nm to 500nm. The mean of the distributions illustrated in Figures 3A and 3B therein yields an average radius of 85-90 nm.…”

Section: A Parameter Valuesmentioning

confidence: 99%

A continuum receptor model of hepatic lipoprotein metabolism

Tindall¹,

Wattis

O’Malley

et al. 2009

Journal of Theoretical Biology

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“…Caveolar bulbs possess a diameter spanning 50-100 nm [10,11], although they can be larger (up to 1 μm) when multiple bulbs combine to form so-called caveosomes and become widespread on cell membranes in subsequent stages after initial wound healing [10,12,13]. Several methods have been explored to visualize their structure, including transmission and scanning electron microscopy, scanning force microscopy, and immunofluorescence, to better understand their involvement in wound healing [7,9,11,13].…”

Section: Introductionmentioning

confidence: 99%

Label-free in vitro visualization and characterization of caveolar bulbs during stimulated re-epithelialization

2014

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Tip-enhanced Raman scattering (TERS) was paired with real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) to characterize lipid aggregates during stimulated re-epithelialization using an in vitro wound healing model. In this study, lipid fluctuations in the plasma membrane of epidermal keratinocytes were studied at multiple time points post-wounding. TERS measurements for the first time were also combined with sample analysis after initial wounding and 24 h of wound healing. This enabled simultaneous visualization and characterization of caveolar bulb distribution during wound healing stages, providing noninvasive insight into their associated lipid structure and coating protein, caveolin, in the nanometer range. The combination of Raman spectroscopy and scanning probe microscopy in TERS gives access to topographic and chemical structure information in a single experiment. It is the intrinsic specificity and sensitivity of TERS that enable this discrete detection of cell surface components on the nanometer scale. In contrast with competing biochemical methods, the applied technique does not interfere with the cellular composition, enabling lipid structure analysis without digestion or detergents, and displayed great potential for future biological in vivo studies.

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Identification of Caveolae-like Structures on the Surface of Intact Cells Using Scanning Force Microscopy

Cited by 13 publications

References 30 publications

Membrane heterogeneity – from lipid domains to curvature effects

Membrane heterogeneity – from lipid domains to curvature effects

A continuum receptor model of hepatic lipoprotein metabolism

Label-free in vitro visualization and characterization of caveolar bulbs during stimulated re-epithelialization

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