Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow

Abstract

Objective-To identify bi-potential precursor cells of erythroid and myeloid development in human bone marrow.Materials and Methods-Cells co-expressing CD13 and CD36 (CD13 + CD36 + ) were investigated by analyzing cell surface marker expression during erythroid development (induced with a combination of cytokines plus erythropoietin [EPO]), or myeloid development (induced with the same cocktail of cytokines plus granulocyte-colony stimulating factor [G-CSF]) of bone marrow derived CD133 cells in liquid cultures. CD13 + CD36 + subsets were also isolated on the 14 th day of cultures and further evaluated for their hematopoietic clonogenic capacity in methylcellulose.Results-Colony-forming analysis of sorted CD13 + CD36 + cells of committed erythroid and myeloid lineages demonstrated that these cells were able to generate erythroid, granulocyte, and mixed erythroid -granulocyte colonies. In contrast, CD13 + CD36 − or CD13 − CD36 + cells exclusively committed to granulocyte/monocyte or erythroid colonies, respectively, but failed to form mixed erythroid -granulocyte colonies; no colonies were detected in CD13 − CD36 − cells with lineagesupporting cytokines. In addition, our data confirmed that EPO induced both erythroid and myeloid commitment, while G-CSF only supported the differentiation of the myeloid lineage.Conclusions-The present data identify some CD13 + CD36 + cells as bi-potential precursors of erythroid and myeloid commitment in normal hematopoiesis. They provide a physiological explanation for the cell identification of myeloid and erythroid lineages observed in hematopoietic diseases. This unique fraction of CD13 + CD36 + cells may be useful for further studies on regulating erythroid and myeloid differentiation during normal and malignant hematopoiesis.

Prospective isolation of human erythroid lineage-committed progenitors

“…CD71 + CD105 − CMPs generated more cells corresponding to E-MEPs and EPs in addition to some CD71 (Fig. 1); thus, it could be that CD71 + CD36 + CD105 − cells within the original CMP and/or MEP fractions are earlier committed E progenitors (e.g., pre-BFU-E), although CD36 is reported to be expressed also on MegKs, monocytes (49), and/or a part of CD13 + CD133 + bipotent myeloerythroid progenitors (50). Moreover, as shown in Fig.…”

The dominant negative β isoform of the glucocorticoid receptor is uniquely expressed in erythroid cells expanded from polycythemia vera patients

“…Differential counts were performed on May-Grünwald-Giemsa-stained cytospin preparations by counting at least 200 cells on randomly selected fields. For flow cytometry, cells were labeled with PE-conjugated CD235a (anti-glycophorin A) and either FITC-conjugated CD36 (an antigen on the thrombospondin receptor 34 ) or CD45 (all from BD Biosciences). Dead cells were identified by staining with propidium iodide (5 g/mL; Sigma-Aldrich).…”

Significance of Alanine Aminopeptidase N (APN) in Bile in the Diagnosis of Acute Cellular Rejection After Liver Transplantation

“…1A). APN is a 150-kD transmembrane protein localized in the bile canaliculi, epithelia of the bile ducts, apical membranes of hepatocytes, mucosal cells of the gall bladder [35], peripheral blood monocytes, granulocytes [36], immature myeloid cells, epithelial cells of the intestine, synaptic membranes in the central nervous system, fibroblasts, endothelial cells, and the brush border membranes of the proximal renal tubular cells [6][7][8]. APN plays a pathologic role in cholelithiasis [35], biliary atresia in infants [37], and cytomegalovirus infection [38].…”