Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins

Huang, Jinming; Wang, X.G.; Jiang, Qiang; Sun, Yan; Yang, Chunhong; Ju, Zhihua; Hao, Hai-Sheng; Wang, C.F.; Zhong, Jun; Zhu, Huabin

doi:10.4238/gmr.15027932

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Mastitis is the most common disease in dairy cows. It has high incidence and prevalence within dairy herds ranging from 20 to 60% and causing more than $350 billion in losses in the dairy industry worldwide [ 1 , 2 ]. Mastitis is an inflammation of the mammary glands in the udders of dairy cows that is mainly caused by infection with pathogenic microorganisms such as Streptococcus agalactiae , Escherichia coli and Staphylococcus aureus .…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of bovine mammary epithelial cells after in vitro incubation with S. agalactiae: potential biomarkers

Tong

Sun

Zhang

et al. 2020

Vet Res

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Streptococcus agalactiae is one of the causative agents of subclinical mastitis, a common disease of dairy cows that causes great economic losses in the industry worldwide. It is thought that pathology is mainly due to inflammatory damage of bovine mammary epithelial cells (bMECs); however, the mechanism by which S. agalactiae damages the bMECs is not clear. The aim of this study was to evaluate the inflammatory effects of S. agalactiae on bMECs and the resulting changes in protein profiles. The bMECs were incubated with S. agalactiae for different times and assayed for cell viability by MTT assay, apoptosis by annexin V and propidium iodide dual staining, and morphological and ultrastructural changes by scanning and transmission electron microscopy. Quantitative real-time PCR was used to determine the effect of S. agalactiae on expression of mRNA of inflammatory factors in bMECs and protein levels were quantitated by liquid chromatography/mass spectrometry. Exposure to S. agalactiae significantly decreased the cell viability and triggered apoptosis, as well as up-regulating TNF-α, IL-1β and IL-6 mRNA, and inhibiting IL-8 expression. S. agalactiae also induced morphological and ultrastructural changes. Furthermore, we identified 325 up-regulated and 704 down-regulated proteins in the treated vs control group. All significant differentially expressed proteins (DSEPs) were classified into three major areas by function: biological processes, cellular components and molecular functions. These differentially expressed proteins included enzymes and proteins associated with various metabolic processes and cellular immunity. Pathway enrichment analysis showed that eight down-regulated signaling pathways were significantly enriched. Exposure to even subclinical levels of S. agalactiae can lead to inflammation and bMEC damage. Our data suggest some possible molecular mechanisms for the harmful effects of subclinical mastitis in dairy cows.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of bovine mammary epithelial cells after in vitro incubation with S. agalactiae: potential biomarkers

Tong

Sun

Zhang

et al. 2020

Vet Res

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“…ncbi.mlm.nih.gov). Gen CD14 memiliki peran penting pada infeksi jaringan ambing yang disebabkan oleh bakteri gram negatif (Huang et al 2016). Cluster Diferensiasi 14 (CD14) terletak di monosit, makrofag, neutrofil, dan sel plasma.…”

Section: Pendahuluanunclassified

Ekspresi dan Pathway Analisis Gen CD14 dan IL10 pada Sapi Perah yang Terinfeksi Mastitis Subklinis

Widiono¹,

Gunawan²,

Sumantri³

et al. 2019

JIPTHP

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Mastitis is an inflammation of internal udder tissue caused by bacteria, it decrease milk production both in quality and quantity. This study was aimed to identify expression and pathway analysis of gene CD14 and IL10 as resistance genes in dairy infected with subclinical mastitis. Sample were taken from milk and blood 10 healthy dairy and 10 dairy containing subclinical mastitis used for CMT and RNA isolation used for gene expression analysis. The result showed that expression in healthy dairy CD14 gene had a higher expression (P<0.05). In contrast the IL10 gene had a high expression in infected dairy with subclinical mastitis (P<0.05). CD14 was an early signal receptor that activates a complex nonspecific immune response to induce various immune characteristics such as the production of inflammatory cytokines and chemokines, the production of antimicrobial peptides and so on destroy pathogens. The high expression of IL10 in infected dairy with subclinical mastitis suggested that immune responses were not functioning properly considered due to inhibiton of cytokine transduction signals by excessive production of IL10.

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Bovine neutrophils in health and disease

Bassel

Caswell

2018

Cell Tissue Res

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Bovine neutrophils have similarities to those of other species with respect to mechanisms of their activation and migration into tissue, modulation of immune responses and the balance between microbial killing and host tissue damage. However, bovine neutrophils have biochemical and functional differences from those of other species, which may yield insights about the comparative biology of neutrophils. Neutrophils play protective and harmful roles in the infectious diseases of cattle that occur at times of transition: respiratory disease in beef calves recently arrived to feedlots and mastitis and other diseases of postparturient dairy cows. An important research focus is the mechanisms by which risk factors for these diseases affect neutrophil function and thereby lead to disease and the prospect of genetic or pharmacologic improvement of disease resistance. Further, in keeping with the One Health paradigm, cattle can be considered a model for studying the role of neutrophils in naturally occurring diseases caused by host-adapted pathogens and are thus an intermediary between studies of mouse models and investigations of human disease. Finally, the study of bovine neutrophils is important for agriculture, to understand the pathogenesis of these production-limiting diseases and to develop novel methods of disease prevention that improve animal health and reduce the reliance on antimicrobial use.

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Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins

Cited by 6 publications

References 30 publications

Proteomic analysis of bovine mammary epithelial cells after in vitro incubation with S. agalactiae: potential biomarkers

Proteomic analysis of bovine mammary epithelial cells after in vitro incubation with S. agalactiae: potential biomarkers

Ekspresi dan Pathway Analisis Gen CD14 dan IL10 pada Sapi Perah yang Terinfeksi Mastitis Subklinis

Bovine neutrophils in health and disease

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