Identification of CD3ɛ, CD4, CD8β splice variants of Atlantic salmon

Maisey, Kevin; Toro-Ascuy, Daniela; Montero, Ruth; Reyes-López, Felipe E.; Imarai, Mónica

doi:10.1016/j.fsi.2011.07.022

Cited by 9 publications

(10 citation statements)

References 60 publications

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“…This is in agreement with past reports showing upregulation of CD4-1 transcripts in lymphoid organs of salmon and trout in response to immunogenic stimuli (16,(48)(49)(50)(51)(52)(53)(54), usually interpreted as an indicator of CD4 + T cell proliferation. Proliferation also has been reported for CD4-1 + cells isolated from ginbuna carp during allogeneic MLR as well as after Ag-specific stimulation (18).…”

Section: Discussionsupporting

confidence: 93%

“…The CD4-1 molecule contains four Ig-like domains (D1-D4), a transmembrane domain, and a cytoplasmic domain (13). Three-dimensional in silico modeling of salmon CD4-1 demonstrated that folding and MHC interaction is highly analogous to that described in mammals during T cell recognition (16). A second CD4-like gene (called CD4-2 or CD4-rel) was first identified in the sea lamprey (Petromyzon marinus) (17) and then in rainbow trout and catfish (13,15).…”

Section: T He Cd4mentioning

confidence: 97%

“…The potentially immunogenic peptide sequence was chosen based on the molecular modeling of the two most distal domains (D1 and D2) of trout CD4-1 using a similar approach as detailed elsewhere (16). As these domains fold as antiparallel b barrels, an attribute of Ig-like domains (Fig.…”

Section: Cd4-1 + T Cells In Troutmentioning

confidence: 99%

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Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

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This study reports the isolation and functional characterization of rainbow trout (Oncorhynchus mykiss) CD4-1+ T cells and the establishment of an IL-15–dependent CD4-1+ T cell line. By using Abs specific for CD4-1 and CD3ε it was possible to isolate the double-positive T cells in spleen and head kidney. The morphology and the presence of transcripts for T cell markers in the sorted CD4-1+CD3ε+ cells were studied next. Cells were found to express TCRα, TCRβ, CD152 (CTLA-4), CD154 (CD40L), T-bet, GATA-3, and STAT-1. The sorted CD4-1+ T cells also had a distinctive functional attribute of mammalian T lymphocytes, namely they could undergo Ag-specific proliferation, using OVA as a model Ag. The OVA-stimulated cells showed increased expression of several cytokines, including IFN-γ1, IL-4/13A, IL-15, IL-17D, IL-10, and TGF-β1, perhaps indicating that T cell proliferation led to differentiation into distinct effector phenotypes. Using IL-15 as a growth factor, we have selected a lymphoid cell line derived from rainbow trout head kidney cells. The morphology, cell surface expression of CD4-1, and the presence of transcripts of T cell cytokines and transcription factors indicated that this is a CD4-1+ T cell line. To our knowledge, this is the first demonstration of the presence of CD4-1+CD3ε+ T cells in salmonids. As in mammals, CD4-1+ T cells may be the master regulators of immune responses in fish, and therefore these findings and the new model T cell line developed will contribute to a greater understanding of T cell function and immune responses in teleost fish.

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Section: Discussionsupporting

confidence: 93%

Section: T He Cd4mentioning

confidence: 97%

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Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

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“…Furthermore, changes affecting predicted exonic splicing enhancer or exonic splicing silencer motif may also compromise the gene function (Fairbrother et al, 2002;Cartegni et al, 2003;Fairbrother et al, 2004;Wang et al, 2004). Importantly, the link between alternative splicing on immunerelated genes in Atlantic salmon has been previously proposed using IPNV (Maisey et al, 2011), suggesting that SNPs affecting these regulatory processes could play a relevant role on the host immune response. Alterations in the immune response associated to SNP located on untranslated regions have been reported in mammals including ccl19 (Muinos-Gimeno et al, 2009;Elton et al, 2010;Cai et al, 2014), itb2 (Bachmann et al, 1997;Mueller et al, 2004;Zhao et al, 2013), and mhc-I (Cree et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar)

Vallejos‐Vidal¹,

Reyes-Cerpa²,

Rivas‐Pardo³

et al. 2020

Front. Genet.

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Single-nucleotide polymorphisms (SNPs) are single genetic code variations considered one of the most common forms of nucleotide modifications. Such SNPs can be located in genes associated to immune response and, therefore, they may have direct implications over the phenotype of susceptibility to infections affecting the productive sector. In this study, a set of immune-related genes (cc motif chemokine 19 precursor [ccl19], integrin b2 (itb2, also named cd18), glutathione transferase omega-1 [gsto-1], heat shock 70 KDa protein [hsp70], major histocompatibility complex class I [mhc-I]) were analyzed to identify SNPs by data mining. These genes were chosen based on their previously reported expression on infectious pancreatic necrosis virus (IPNV)-infected Atlantic salmon phenotype. The available EST sequences for these genes were obtained from the Unigene database. Twenty-eight SNPs were found in the genes evaluated and identified most of them as transition base changes. The effect of the SNPs located on the 5'-untranslated region (UTR) or 3'-UTR upon transcription factor binding sites and alternative splicing regulatory motifs was assessed and ranked with a low-medium predicted FASTSNP score risk. Synonymous SNPs were found on itb2 (c.2275G > A), gsto-1 (c.558G > A), and hsp70 (c.1950C > T) with low FASTSNP predicted score risk. The difference in the relative synonymous codon usage (RSCU) value between the variant codons and the wild-type codon (DRSCU) showed one negative (hsp70 c.1950C > T) and two positive DRSCU values (itb2 c.2275G > A; gsto-1 c.558G > A), suggesting that these

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“…In teleosts, CD3 gene has been covered in a few species, such as flounder, fugu and salmon (Alabyev et al, ; Araki, Suetake, Kikuchi, & Suzuki, ; Park, Hirono, Enomoto, Nam, & Aoki, ; Park, Hirono, & Aoki, ; Shang, Sun, Hu, Wang, & Guo, ). Most of the studies have focused on the structure of CD3 (Shang et al, ; Maisey, Toro‐Ascuy, Montero, Reyes‐Lopez, & Imarai, ) and a few on the protein and cellular levels. In addition, like other CD3 chains, CD3ε also has a signal peptide, transmembrane region and highly variable extracellular region and cytoplasmic tail regions.…”

Section: Introductionmentioning

confidence: 99%

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

Kong

et al. 2020

Aquac Res

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CD3 is an important membrane molecule of mature T cells. TCR‐CD3 complex plays important roles in the activation and signal transduction of T cells. In this study, the CD3ε (OnCD3ε) gene was cloned from Nile tilapia (Oreochromis niloticus), and its tissue distribution was detected by qPCR. The expression changes in CD3ε were analysed after three challenges (S. agalactiae, A. hydrophila and poly (I:C)) in vivo and in vitro. The open reading frame (ORF) of OnCD3ε contains 531 bp, encoding 176 amino acids, which found that OnCD3ε consists of conserved amino acid residues and motifs (cysteine residues, CXXC motif, immunoreceptor tyrosine‐based activation motifs and PxPDY). In addition, tissue distribution displayed that OnCD3ε has the highest expression in thymus and significant expression level in mucosal immune‐related tissues (intestine and gills). In vivo, all three challenges could significantly up‐regulate the expression of CD3ε in the head kidney and spleen. For further in vitro assays, after poly (I:C) stimulation, OnCD3ε appeared significant up‐regulation at 3 hr post stimulation, and two pathogenic bacteria also induce significant up‐regulation of OnCD3ε in spleen leucocytes (12 hr). These results suggested that OnCD3ε was likely to get involved in host immune response against pathogenic challenges.

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Identification of CD3ɛ, CD4, CD8β splice variants of Atlantic salmon

Cited by 9 publications

References 60 publications

Isolation and Characterization of Salmonid CD4+ T Cells

Isolation and Characterization of Salmonid CD4+ T Cells

Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar)

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

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