Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts

Kim, Jin-Kyeoung; Ryll, Roland; Ishizuka, Yoshiko; Kato, Souichiro

doi:10.1016/s0378-1119(00)00414-5

Cited by 22 publications

(27 citation statements)

References 24 publications

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“…Morphologically, these H2RSP-expressing stromal cells were infiltrated inflammatory cells and proliferating fibroblastic (myofibroblastic) cells. In this regard, IMUP-1, an identical peptide with H2RSP, has been identified as one of the mRNAs upregulated in SV40-immortalized fibroblasts compared to senescent WI-38 fibroblasts [8]. This characteristic of IMUP-1 may be consistent with our present observation that H2RSP was upregulated in proliferating fibroblastic cells in response to tissue injury.…”

Section: Discussionsupporting

confidence: 91%

“…Thus, it is unlikely that H2RSP is directly involved in the regulation of HGF activation, at least in mice, and so far, biological roles of H2RSP have been poorly understood. H2RSP is identical to immortalization-upregulated protein-1 (IMUP-1), which was identified as one of the mRNAs upregulated in SV40-immortalized fibroblasts compared with senescent fibroblasts [8]. Thus, H2RSP/IMUP-1 may have a role in cellular proliferation.…”

Section: Introductionmentioning

confidence: 99%

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Nuclear translocation of H2RSP is impaired in regenerating intestinal epithelial cells of murine colitis model

et al. 2005

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Hepatocyte growth factor activator inhibitor type 2-related small peptide (H2RSP) is a recently identified nuclear peptide that is abundantly expressed in the gastrointestinal tract. In this study, we analyzed the expression of H2RSP in normal and injured intestinal mucosa in a murine experimental colitis induced by oral administration of 2.5% dextran sodium sulfate. Results of immunohistochemistry and in situ hybridization showed that H2RSP was expressed predominantly in the epithelium of normal intestine. Whereas H2RSP was localized in the cytoplasm of cells in the crypt, it was translocated into the nuclei of the surface epithelial cells. In injured intestine, H2RSP was detected in the cytoplasm of regenerating epithelial cells, and the nuclear translocation was impaired even in the surface epithelium. However, the mRNA level was not significantly altered in these cells by real-time reverse transcription-polymerase chain reaction using total RNAs obtained from the fractionated mucosal tissue samples prepared by laser-captured microdissection technique. On the other hand, H2RSP mRNA was significantly upregulated in the stromal cells of injured intestinal mucosa compared with those in normal mucosa, which shows cytoplasmic localization of H2RSP. These circumstantial evidences suggest that the nuclear translocation of H2RSP may be related to a signaling involved in the transition from cellular proliferation to differentiation.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Nuclear translocation of H2RSP is impaired in regenerating intestinal epithelial cells of murine colitis model

et al. 2005

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“…The genes would be regulated independently by distinct transcription factors, although these genes were mapped very closely to each other and chimeric mRNAs were transcribed from both genes in several tissues. Just before submission of this article, an identical mRNA with H2RSP, namely the immortalization-upregulated protein-1 (IMUP-1) has been reported as one of mRNAs upregulated in SV40-immortalized compared to senescent WI-38 fibroblasts (17). Further comparable reporter analyses would clarify the transcriptional regulation of these genes.…”

Section: Discussionmentioning

confidence: 93%

Identification of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2)-Related Small Peptide (H2RSP): Its Nuclear Localization and Generation of Chimeric mRNA Transcribed from both HAI-2 and H2RSP Genes

Itoh

Kataoka

Yamauchi

et al. 2001

Biochemical and Biophysical Research Communications

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“…15 18 To date, little is known about the biological role of H2RSP in vivo. As IMUP-1, an identical protein to H2RSP, was up regulated in SV40-immortalised compared with senescent fibroblasts, 16 H2RSP/IMUP-1 may thus have a role in cellular proliferation or differentiation.…”

mentioning

confidence: 96%

“…14 15 H2RSP is identical to immortalisation up regulated protein-1 (IMUP-1), which has been identified as one of the mRNAs up regulated in SV40-immortalised compared with senescent fibroblasts. 16 The human H2RSP/ IMUP-1 gene consists of four exons spanning approximately 1 kbp, and is located 11 kbp downstream of HAI-2 gene (19q.13.11; brief gene organisation is shown in fig 1C). 15 17 An engineered expression of the deleted series of H2RSP cDNAs fused to enhanced green fluorescent protein in HeLa cells disclosed the nuclear localisation signal in the lysine-rich region of exon 4.…”

mentioning

confidence: 99%

Enhanced expression of hepatocyte growth factor activator inhibitor type 2-related small peptide at the invasive front of colon cancers

Uchiyama

Itoh²,

Naganuma³

et al. 2007

Gut

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Background: Hepatocyte growth factor activator inhibitor type 2-related small peptide (H2RSP) is a small nuclear protein abundantly expressed in the gastrointestinal epithelium. However, its functions remain unknown. Aims: To investigate the expression and localisation of H2RSP in normal, injured and neoplastic human intestinal tissue. Methods: Immunohistochemical examination and in situ hybridisation for H2RSP were performed using normal and diseased intestinal specimens. Its subcellular localisation and effects on the cellular proliferation and invasiveness were examined using cultured cells. Results: In the normal intestine, H2RSP was observed in the nuclei of surface epithelial cells and this nuclear localisation was impaired in regenerating epithelium. In vitro, the nuclear translocation of H2RSP was observed along with increasing cellular density, and an overexpression of H2RSP resulted in a reduced growth rate and enhanced invasiveness. H2RSP expression was down regulated in well-differentiated colorectal adenocarcinomas. However, a marked up regulation of the cytoplasmic H2RSP immunoreactivity was observed in cancer cells at the invasive front. These cells showed low MIB-1 labelling, an enhanced p16 expression and nuclear b-catenin. The number of H2RSP-positive cells in the invasive front of welldifferentiated adenocarcinomas was considerably higher in the cases with lymph node metastases than in node-negative ones. Conclusion: In the normal intestine, the nuclear accumulation of H2RSP is a marker of differentiated epithelial cells. Although H2RSP was down regulated in colorectal adenocarcinomas, a paradoxical up regulation was observed in actively invading carcinoma cells. H2RSP immunoreactivity at the invasive front may serve as a marker of invasive phenotype of well-differentiated colon cancers.

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Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts

Cited by 22 publications

References 24 publications

Nuclear translocation of H2RSP is impaired in regenerating intestinal epithelial cells of murine colitis model

Nuclear translocation of H2RSP is impaired in regenerating intestinal epithelial cells of murine colitis model

Identification of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2)-Related Small Peptide (H2RSP): Its Nuclear Localization and Generation of Chimeric mRNA Transcribed from both HAI-2 and H2RSP Genes

Enhanced expression of hepatocyte growth factor activator inhibitor type 2-related small peptide at the invasive front of colon cancers

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