Identification of Cell Proliferation Zones, Progenitor Cells and a Potential Stem Cell Niche in the Intervertebral Disc Region

Henriksson, Helena Barreto; Thornemo, Maria; Karlsson, Camilla; Hägg, Olle; Junevik, Katarina; Lindahl, Anders; Brisby, Helena

doi:10.1097/brs.0b013e3181a95ad2

Cited by 176 publications

(159 citation statements)

References 49 publications

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“…The expression of these markers decreased during aging, but if they are present in mature discs (>18 years), especially galectin-3, this could facilitate disc degeneration. This finding contradicts previous studies, as notochordal cells have a pivotal role in tissue development and regeneration (Henriksson et al, 2009;Minogue et al, 2010b;Pattappa et al, 2012;Risbud et al, 2010;Sakai et al, 2009). Like the cellular response to hydrostatic pressure, in vitro models of NP cells might not reflect in vivo conditions regarding cell-tissue interaction and consequent notochordal cell behavior.…”

Section: Cellular Differentiationcontrasting

confidence: 56%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

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The unique properties of the intervertebral disc (IVD) are evident in its structural complexity and functional importance for spinal support and stability. The contributions of the different cellular and extracellular components to the function of the IVD depend on their distinctive molecular features and pathways. Disruption of these molecular pathways influences the pathological changes involved in IVD degeneration. Therefore, the molecular features of the IVD have been the focus of interest for many researchers seeking to elucidate its normal functioning, potential pathologies, and appropriate therapies. The aim of the present article is to review the molecular aspects of IVD development, specific cellular markers, and the interactions between cellular and extracellular components responsible for homeostasis, degeneration and potential therapies. The literature available via PubMed and Google Scholar was reviewed and the relevant references in review articles were searched manually. Spine

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Section: Cellular Differentiationcontrasting

confidence: 56%

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Aker¹,

Ghannam²,

Alzuabi³

et al. 2017

The Spine Scholar

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“…Previous in vitro studies suggested that the intervertebral disc is a tissue with small cell proliferation and regeneration capacity both in annulus fibrosus and nucleus pulposus [44][45][46]. Cell proliferation and regeneration capacity can be increased by external stimulation to promote the restoration of intervertebral discs.…”

Section: Discussionmentioning

confidence: 99%

Effects of resting modes on human lumbar spines with different levels of degenerated intervertebral discs: a finite element investigation

Fan

Gong

Qiu

et al. 2015

BMC Musculoskelet Disord

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Background: The negative effect of long-term working load on lumbar is widely known. However, insertion of different resting modes on long-term working load, and its effects on the lumbar spine is rarely studied. The purpose of this study was to investigate the biomechanical responses of lumbar spine with different levels of degenerated intervertebral discs under different working-resting modes. Methods: Four poroelastic finite element models of lumbar spinal segments L2-L3 with different grades of disc degeneration were developed. Four different loading conditions represented four different resting frequencies, namely, no rest, one-time long rest, three-time moderate rests, and five-time short rests, on the condition that the total resting time was the same except in the no rest mode. Loading amplitudes of diurnal activities included 100 N, 300 N, and 500 N. Results: With increasing resting frequency, the axial effective stress and fluid loss decreased, whereas the pore pressure and radial displacement increased. Under different resting frequencies, the changing rate of each biomechanical parameter was different. Conclusions: Under a situation of fixed total resting time, high resting frequency was advisable. If sufficient resting frequency was unavailable for healthy people as well as patients with mildly and moderately degenerated intervertebral discs, they could similarly benefit from relatively less resting frequencies. However, one-time rest will not be useful in cases where intervertebral discs were seriously degenerated. Reasonable working-resting modes for different degrees of disc degeneration, which could assist patients achieve a better restoration, were provided in this study.

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“…Using a panel of markers validated by the International Society for Cell Therapy as bone marrow MSC markers (CD90?, CD73?, CD105?, CD166?, CD45-, CD34-, CD14-, HLA-DR-), Risbud and colleagues [65] identified cells within human degenerate discs, that were capable of undergoing differentiation along the chondro-, osteo-and adipogenic lineages, while Blanco and colleagues [66] identified cells capable of chondro and osteogenic differentiation. Using BrdU labelling, Henriksson and colleagues [67] located in vivo a population of progenitor cells in the AF border that showed proliferation in a stem cell niche-like pattern.…”

Section: T (Brachyury) Ratmentioning

confidence: 99%

An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration

2014

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Cell-based regenerative medicine therapies have been proposed for repairing the degenerated intervertebral disc (a major cause of back pain). However, for this approach to be successful, it is essential to characterise the phenotype of its native cells to guarantee that implanted cells differentiate and maintain the correct phenotype to ensure appropriate cell and tissue function. While recent studies have increased our knowledge of the human nucleus pulposus (NP) cell phenotype, their ontogeny is still unclear. The expression of notochordal markers by a subpopulation of adult NP cells suggests that, contrary to previous reports, notochord-derived cells are retained in the adult NP, possibly coexisting with a second population of cells originating from the annulus fibrosus or endplate. It is not known, however, how these two cell populations interact and their specific role(s) in disc homeostasis and disease. In particular, notochordal cells are proposed to display both anabolic and protective roles; therefore, they may be the ideal cells to repair the degenerate disc. Thus, understanding the ontogeny of the adult NP cells is paramount, as it will inform the medical and scientific communities as to the ideal phenotype to implant into the degenerate disc and the specific pathways involved in stem cell differentiation towards such a phenotype.

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Identification of Cell Proliferation Zones, Progenitor Cells and a Potential Stem Cell Niche in the Intervertebral Disc Region

Cited by 176 publications

References 49 publications

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Molecular Biology and Interactions in Intervertebral Disc Development, Homeostasis, and Degeneration, with Emphasis on Future Therapies: A Systematic Review

Effects of resting modes on human lumbar spines with different levels of degenerated intervertebral discs: a finite element investigation

An understanding of intervertebral disc development, maturation and cell phenotype provides clues to direct cell-based tissue regeneration therapies for disc degeneration

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