Identification of chicken FSHR gene promoter and the correlations between polymorphisms and egg production in Chinese native hens

Abstract

ContentsEgg production is an important economic trait in poultry, and it is of great significance to study the key genes and functional SNPs that affect egg laying performance. Follicle‐stimulating hormone (FSH) plays an important physiological role in the reproductive performance of humans and animals by binding to its receptor (FSHR). Studies have shown that there are many transcriptional regulatory elements in the 5′ flanking region of the FSHR gene that interact with transcription factors to regulate FSHR transcription. In this study, DNA sequencing was used to identify SNPs in the FSHR promoter sequence in both Dongxiang and Suken chickens. To detect the activity of the chicken FSHR gene promoter, we analysed the characteristics of the sequence and constructed three deletion vectors. We confirmed that the region (−18/−544) was the core promoter. Furthermore, five polymorphisms, including a 200‐bp indel at −869, C−1684T, C−1608T, G−368A and T−238A, were detected in both the Dongxiang and Suken chickens. The age at first egg (AFE) for different genotype of −869 indel in Suken chicken was significantly different (p < 0.01). For SNP C−1684T in Dongxiang chickens, the CC genotype had higher egg number at 43 weeks of age (E43) than that of the TC genotype (p < 0.05). For SNP C−1684T in Suken chickens, the TC genotype had higher AFE than that of the CC genotype (p < 0.05). For SNP C−1608T in Suken chickens, the CC genotype had higher AFE than that of the TC genotype (p < 0.05). For SNP G−368A in Suken chickens, the AG genotype had higher AFE than that of the GG genotype (p < 0.05).

Genetic polymorphism in core promoter sequence of ACTRIIB gene and association analysis with growth traits in chicken

“…The SNP association analysis revealed that apart from SNP 1, all the remaining SNPs had significant association with growth traits in both lines of chicken, suggesting the intrigue role of these Likewise, Bhattacharya et al, (2016) found that exons 2 and 4 of activin receptor 2A and activin receptors 2B were polymorphic and strongly associated with certain carcass traits in chicken population. Congruent with our findings, earlier studies reported significant association between single nucleotide polymorphisms and growth traits in innumerable genes among various chicken breeds (Bhattacharya, Chatterjee, Dange, & Bhanja, 2019;Li et al, 2019;Trevisoli et al, 2018).…”

Skeletal Muscle Transcriptome Analysis of Hanzhong Ma Duck at Different Growth Stages Using RNA-Seq

“…SNP is a kind of efficient genetic marker based upon the variability at the nucleotide level and it has been widely used in genetic studies and breeding applications in animal species [ 24 , 25 ]. SNPs are more abundant in organisms, which have greater stability over generations and are more accurately genotyped.…”

Effect of a Diet Supplemented with the Moringa oleifera Seed Powder on the Performance, Egg Quality, and Gene Expression in Japanese Laying Quail under Heat-Stress

“…Conversely, the expressions of selected genes (ESR2 and FSHR) decreased in the T1 and T2 groups, possibly due to heat stress. Li et al [11] have suggested that heat stress induces granulosa cell apoptosis and reduces steroidogenic gene mRNA expressions. Previous studies on mammals have demonstrated that heat stress inhibits the development of ovarian follicles and tends to cause ovarian regression [54,55].…”

“…Heat stress directly affects ovarian function by altering folliculogenesis and the secretion of ovarian hormones, such as prolactin, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) [9]. Ovarian functions and folliculogenesis are mainly controlled by various genes, such as steroidogenic acute regulatory protein (STAR), inhibin subunit alpha (INHA), bone morphogenetic protein 15 (BMP-15), follicle-stimulating hormone receptor (FSHR), and estrogen receptors 1 and 2 (ESR1 and ESR2) [10][11][12][13][14].…”