Identification of clinical yeasts by Vitek MS system compared with API ID 32 C

Durán-Valle, María Teresa; Sanz-Rodríguez, Nuria; Muñoz-Paraíso, Carmen; Almagro-Moltó, María; Gómez-Garcés, José Luís

doi:10.1093/mmy/myt036

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…Fourteen studies investigated the Vitek MS for yeast identification performance, of which three were financially supported by BioMérieux . All studies except one used a on‐target formic acid extraction.…”

Section: Comparison Of the Four Maldi‐tof Ms Platforms Concerning Funmentioning

confidence: 99%

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Performance of MALDI‐TOF MS platforms for fungal identification

Cassagne

Normand

L’Ollivier

et al. 2016

Mycoses

146

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Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) is increasingly used by clinical microbiology laboratories to cope with the need for rapid, cost-effective and accurate identification of microorganisms. Several research teams have recently succeed in identifying moulds using MALDI-TOF MS, which was first adapted to bacteria, then to yeast identification. Since 2004, different commercial firms have released several ready-to-use MALDI-TOF MS platforms. This review describes the similarities and differences between the commercially available systems. In two parts, we first describe and compare the preprocessing and identification steps between the platforms and then compare the identification efficacy of yeast, moulds and dermatophytes species.

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Section: Comparison Of the Four Maldi‐tof Ms Platforms Concerning Funmentioning

confidence: 99%

“… lowered the confidence level cut‐off to 60%. Only one study used a drastic cut‐off for the confidence value at 95% . The correct identification rates ranged from 84.3% (Mancini et al .…”

Section: Comparison Of the Four Maldi‐tof Ms Platforms Concerning Funmentioning

confidence: 99%

Performance of MALDI‐TOF MS platforms for fungal identification

Cassagne

Normand

L’Ollivier

et al. 2016

Mycoses

146

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“…The material was cultivated on Sabouraud dextrose agar for 72 h at 37 • C. Candida spp. was identified based on germ-tube formation, chlamydospore production, and carbohydrate assimilation using the API ID 32C system (bioMerieux, Marcy l'Etoile, France) [21][22][23].…”

Section: Cultivation and Identification Of Candida Sppmentioning

confidence: 99%

Association between Vitamin D and Candida-Associated Denture Stomatitis

et al. 2020

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Candida-associated denture stomatitis (CADS) is a fungal infection affecting 60–65% of denture wearers. Its etiology is complex and multifactorial and often associated with host immunodeficiency. Evidence exists that vitamin D has potential immunomodulatory and anti-inflammatory effects. The aim of this case–control study was to assess the association between vitamin D levels and CADS. The study included 32 complete denture wearers with CADS and 32 sex- and age-matched complete denture wearers without CADS. The patients were clinically examined, and the severity of denture stomatitis was assessed according to Newton’s classification scale. The serum vitamin D level was determined via the use of an electrochemiluminescence assay. The vitamin D level in the CADS group and control group was 54.68 ± 17.07 and 56.82 ± 17.75 nmol/L, respectively. There was no significant difference between the groups (p = 0.622). Univariate logistic regression analysis showed that the presence of CADS was not associated with hypovitaminosis D (odds ratio (OR) = 1.44; 95% confidence interval (CI) = 0.37–5.54). It can be concluded that vitamin D is not associated with CADS and does not play a significant role in host susceptibility to CADS. This finding suggests that vitamin D screening is not indicated routinely in patients with Candida-associated denture stomatitis.

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“…Conventional identification of yeast is based on morphology and biochemical tests using its ability to metabolize different sugars and related compounds. Such methods take approximately 24‐72 hours and often fail to accurately identify emerging or cryptic species . Molecular identification by sequencing of genomic regions is considered a standard method for identifying yeast to the species level .…”

Section: Introductionmentioning

confidence: 99%

“…However, molecular identification remains expensive, time‐consuming, and relatively labor‐intensive to perform in clinical microbiology laboratories . Due to the above limitations, the introduction of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has been currently in the spotlight and has greatly improved the routine identification of yeast as well as bacteria at species level in the last few years . There are three commercially available MALDI‐TOF MS systems, the Microflex Biotyper (Bruker Daltonics, Bremen, Germany), the VITEK MS (bioMérieux, Marcy l'Etoile, France), and the Andromas (Andromas, Paris, France) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a new matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry system for the identification of yeast isolation

Lee

Park

et al. 2018

Clinical Laboratory Analysis

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Background: Currently, three commercial in vitro diagnostic matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are widely used in clinical laboratories. The ASTA MicroIDSys system (ASTA Inc, South Korea) is a newly developed MALDI-TOF MS system used for the identification of pathogenic microorganisms. In the present study, we assessed the performance of the ASTA MALDI-TOF MS system for the identification of pathogenic yeast from clinical samples. Methods:We tested 284 clinical yeast isolates from various clinical specimens using ASTA MALDI-TOF MS, and the results were compared with those using molecular sequencing of the ITS or D1-D2 regions of rDNA and biochemical assays.Results: A total of 284 isolates were tested and found to be distributed across 14 species including Candida albicans (n = 100) and other yeast species (n = 184). ASTA MALDI-TOF MS correctly identified 95.1% (270/284) of the yeast species compared to molecular sequencing. Among them, 262 isolates showed acceptable MALDI-TOF MS scores (≥140), and 98.1% (257/262) isolates were identified correctly. In addition, among 22 isolates with a MALDI-TOF MS score <140, 59.1% (13/22) of the isolates showed concordance with molecular typing at the species level. Clustering analysis revealed the effectiveness of the new MALDI-TOF MS system for the identification of yeast species.Conclusions: ASTA MALDI-TOF MS showed high accuracy in the identification of yeast species; it involves facile sample preparation and extraction procedures. ASTA MALDI-TOF MS is expected to be useful for yeast identification in clinical microbiology laboratories due to its reliability and cost-effectiveness. K E Y W O R D S identification, MALDI-TOF MS, sequencing, yeast How to cite this article: Lee H, Park J-H, Oh J, et al. Evaluation of a new matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry system for the identification of yeast isolation. J Clin Lab Anal.

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Identification of clinical yeasts by Vitek MS system compared with API ID 32 C

Cited by 10 publications

References 25 publications

Performance of MALDI‐TOF MS platforms for fungal identification

Performance of MALDI‐TOF MS platforms for fungal identification

Association between Vitamin D and Candida-Associated Denture Stomatitis

Evaluation of a new matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry system for the identification of yeast isolation

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