Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Xie, Li; Zhang, Jingze; Wan, Yao; Hu, Dongwei

doi:10.1631/jzus.b0900174

Cited by 58 publications

(29 citation statements)

References 33 publications

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“…In some cases, the two species occur in the same plant and in the same lesion, showing the coexistence of the two species of Colletotrichum. This has already been observed in other plant species such as strawberry, chilli pepper, peach, apple and citrus (Pileggi et al 2009;Xie et al 2010). However, the factors involved in this association have not been determined.…”

Section: Discussionsupporting

confidence: 59%

Differential Organ Distribution, Pathogenicity and Benomyl Sensitivity of Colletotrichum spp. from Blackberry Plants in Northern Colombia

Rueda-Hernández

Cardona

Cadavid-Restrepo

et al. 2012

Journal of Phytopathology

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Blackberry anthracnose, caused by Colletotrichum spp., is an important disease of cultivated blackberry in the world. In Colombia, it is the number one limiting factor for commercial production. This study was conducted to determine the species of Colletotrichum infecting blackberry plants as well as the organ distribution, pathogenicity and response to benomyl of the isolated strains. Sixty isolates from stems (n = 20), thorns (n = 20) and inflorescences (n = 20) were identified as Colletotrichum acutatum and Colletotrichum gloeosporioides by a species‐specific polymerase chain reaction (PCR). Both Colletotrichum species were found in the same plant but on different organs. Colletotrichum gloeosporioides species predominated in thorn lesions (n = 16) and C. acutatum in stems (n = 15) and inflorescence (n = 15). Pathogenicity assays on detached blackberry organs demonstrated differences between the two species with an average period of lesion development of 8.7 days for C. gloeosporioides and 10.3 days for C. acutatum. Wound inoculated organs had 90% disease development compared to 17.5% in non‐wounded. All C. acutatum isolates (n = 34) were benomyl tolerant, whereas C. gloeosporioides isolates (n = 26) were 30.7% sensitive and 69.2% moderately tolerant. Phylogenetic analysis with ITS sequences of a subset of 18 strains showed that strains classified as C. gloeosporioides had 100% identity to Colletotrichum kahawae, which belongs to the C. gloeosporioides species complex, whereas C. acutatum strains clustered into two different groups, with high similarity to the A2 and the A4 molecular groups. These data demonstrate for the first time the differential distribution of both species complexes in blackberry plant organs and further clarifies the taxonomy of the strains.

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Section: Discussionsupporting

confidence: 59%

Differential Organ Distribution, Pathogenicity and Benomyl Sensitivity of Colletotrichum spp. from Blackberry Plants in Northern Colombia

Rueda-Hernández

Cardona

Cadavid-Restrepo

et al. 2012

Journal of Phytopathology

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“…are worldwide in distribution, causing important diseases in humans, ornamentals and economic crops [5][6][7] and in humid and sub-humid tropics they occur as saprobes, endophytes or pathogens on leaves, stems, flowers and fruits in susceptible crops [8]. Many economically important diseases including anthracnose have been ascribed to attacks from members of the genus [9,10]. Anthracnose is one of the major important fungal diseases which affect the yield and economic production of cowpea [11], causing up to 50% yield reduction [12].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Composition of Seed and Husk of Cowpea (Vigna unguiculata (L.) Walp.) Infected by Colletotrichum destructivum O’Gara in Storage

Amadioha

Nwazuo

2019

ARRB

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Colletotrichum destructivum was isolated from infected seeds and pods of cowpea (Var. IAR-48) with typical symptoms of anthracnose disease. The fungus during the pathogenesis, reduced the protein, fat, ash, crude fibre, carbohydrate, calcium and phosphorus, and increased the amount of iron, sodium, zinc, magnesium and potassium in the infected seed and husk. The carbohydrate, protein and phosphorus contents in the healthy husk reduced from 55.05%, 11.21% and 171.85 mg to 39.94%, 8.92% and 42.92 respectively in the infected sample whereas potassium and sodium contents in the healthy pod increased from 1.03 mg and 78.29 to 2.90 mg and 100.65 mg respectively in the infected husk. The potassium, sodium, magnesium and iron increased from 1292.25 mg, 0.19 mg, 0.09 mg and 11.00 mg in the healthy seeds to 1536.03 mg, 0.28 mg, 0.21 mg and 13.19 mg respectively in the infected seeds. The fungus caused the depletion of phosphorus from 498. 06 mg in the healthy to 430.17 mg in the infected seed, protein from 24.09% to 17.86%, carbohydrate from 57.02% to 34.35%, fat from 1.70% to 1.33% and crude fibre from 3.94% to 2.61%. The average loss of the major nutrient values; protein, carbohydrate and fat were 28.95% and 22.55% for seed and husk respectively after 8 weeks of planting.

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“…Following a phenol/chloroform extraction, the genomic DNA was precipitated by isopropanol in the presence of sodium acetate. Genomic DNA was visualized in 1% agrose gels (0.01 g/ml) after ethidium bromide staining (Xie et al, 2010).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Ultrastructure and phylogeny of Ustilago coicis

Zhang

Guan

Tao

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Ustilago coicis causes serious smut on Coix lacryma-jobi in Dayang Town, Jinyun County, Zhejiang Province of China. In this paper, ultrastructural assessments on fungus-host interactions and teliospore development are presented, and molecular phylogenetic analyses have been done to elucidate the phylogenetic placement of the taxon. Hyphal growth within infected tissues was both intracellular and intercellular and on the surface of fungus-host interaction, and the fungal cell wall and the invaginated host plasma membrane were separated by a sheath comprising two distinct layers between the fungal cell wall and the invaginated host plasma membrane. Ornamentation development of teliospore walls was unique as they appeared to be originated from the exosporium. In addition, internal transcribed spacer (ITS) and large subunit (LSU) sequence data showed that U. coicis is closely related to Ustilago trichophora which infects grass species of the genus Echinochloa (Poaceae).

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Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China

Cited by 58 publications

References 33 publications

Differential Organ Distribution, Pathogenicity and Benomyl Sensitivity of Colletotrichum spp. from Blackberry Plants in Northern Colombia

Differential Organ Distribution, Pathogenicity and Benomyl Sensitivity of Colletotrichum spp. from Blackberry Plants in Northern Colombia

Biochemical Composition of Seed and Husk of Cowpea (Vigna unguiculata (L.) Walp.) Infected by Colletotrichum destructivum O’Gara in Storage

Ultrastructure and phylogeny of Ustilago coicis

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