Identification of common virulence factors present in enterotoxigenic<i>Escherichia coli</i>isolated from diarrhoeal patients in Kolkata, India

Bhakat, D.; Debnath, A. K.; Naik, Roshni; Chowdhury, Goutam; Mukhopadhyay, Asish K.; Chatterjee, Nando K.

doi:10.1111/jam.14090

Cited by 8 publications

(9 citation statements)

References 36 publications

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“…The third prevalent pathotype of this study was ETEC causing moderate-to-severe diarrhea in human. The pro les st/lt and st were the most common toxigenic genotype among ETECs, which agrees with Bhakat et al (2019) [30]. None of the strains carried the lt gene alone, which is in contrast with the global prevalence of the gene where the presence of lt is higher than st or st/lt pro le [31].…”

Section: Discussionsupporting

confidence: 88%

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

Asadi¹,

Ghanbarpour²,

Kalantar-Neyestanaki³

et al. 2021

Preprint

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Background: Intra-intestinal pathogenic strains of Escherichia coli are responsible for mild to severe gastrointestinal lesions, which are mediated by various virulence factors. In this study, we have focused on a comprehensive set of E. coli pathotypes, including STEC, EPEC, EHEC, ETEC, and some hybrid pathotypes including, STEC/ ETEC, EHEC/ ETEC, and EPEC/ ETEC.Methods: Totally, 467 stool samples were obtained from gastrointestinal patients during four years (2016 to 2020). Four pathotypes of E. coli (EPEC, ETEC, EHEC, STEC) were screened due to six virulence genes, including eae, stx1, stx2, st, and lt using the conventional PCR method. Finally, detected E. coli pathotypes were subjected to determine phenotypic and genotypic β-lactam resistance properties.Result: In this study 59/467 (12.63%) strains belonged four pathotypes including STEC (20/59; 50.8%), EPEC (11/59; 18.6%), ETEC (8/59 13.5%), EHEC (5/59; 8.4%) and three novel hybrid pathotypes including STEC/ETEC (3/59; 5%), EHEC/ETEC (1/59; 1.6%), and EPEC/ETEC (1/59; 1.6%). Totally 23/59 (38.9%) isolates were identified as ESBL-producing. Conclusion: Transmission of virulence and antibiotic resistance genes among E. coli strains lead to the emergence of antimicrobial-resistant hybrid pathogenic strains, which is an important health concern. According to food-born and fecal-oral transmission of these E. coli strains, standard methods should be performed to eliminate the possible contamination of food, equipment, and living environment to manure and feces.

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Section: Discussionsupporting

confidence: 88%

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

Asadi¹,

Ghanbarpour²,

Kalantar-Neyestanaki³

et al. 2021

Preprint

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“…In accordance with our findings, a study on the ETEC population in Nepal revealed that CS21 (62.6%) and CS6 (30.2%) were the most prevalent CFs (Margulieux et al, 2018). Another study in our laboratory earlier detected CS21 as the prevalent CF followed by CS6 among clinical ETEC isolates from Kolkata (Bhakat et al, 2018). In Bangladesh, frequently detected CFs were CS5, CS6 and CS1 (Begum et al, 2014).…”

Section: Discussionmentioning

confidence: 79%

Distribution of virulence factors and its relatedness towards the antimicrobial response of enterotoxigenic Escherichia coli strains isolated from patients in Kolkata, India

Mondal

Bhakat

Chowdhury

et al. 2021

J of Applied Microbiology

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Aim: Enterotoxigenic Escherichia coli (ETEC) is one of the most widely recognized diarrhoeal pathogens in developing countries. The advancement of ETEC vaccine development depends on the antigenic determinants of the ETEC isolates from a particular geographical region. So, the aim here was to comprehend the distribution of virulence determinants of the clinical ETEC strains of this region. Additionally, an attempt was made to find any correlation with the antimicrobial response pattern. Methods and results: Multiplex PCR was employed to identify virulence determinants followed by confirmatory singleplex PCR. For observation of antibiotic response, the Kirby-Bauer method was used. Out of 379 strains, 46% of strains harboured both the enterotoxins ST and LT, whereas 15% were LT only. Among the major colonization factors (CFs), CS6 (41%) was the most prevalent followed by CFA/I (35%) and CFA/ III was the lowest (3%). Among the minor CFs, CS21 (25%) was most prevalent, while CS15 showed the lowest (3%) presence. Among the non-classical virulence factors, EatA (69%) was predominant. ETEC strains harbouring CS6 showed resistance towards the commonly used drug Ciprofloxacin (70%). Conclusion: CS6 and elt+est toxin genes co-occurred covering 51% of the isolates. CS21 was found in most strains with est genes (43%). EatA was found to occur frequently when ST was present alone or with LT. CS6-harbouring strains showed an independent correlation to antimicrobial resistance. Significance and Impact of the Study: This study would aid in identifying the commonly circulating ETEC isolates of Kolkata, India, and their prevalent virulence determinants. Knowledge of antibiotic resistance patterns would also help in the appropriate use of antibiotics. Furthermore, the study would aid in identifying the multivalent antigens suitable for region-specific ETEC vaccines with maximum coverage.

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“…The four genes were selected out of many genes reported because of high occurance of these genes in the dirrheagenic E.coli isolates. Recently, the isolates having elt and est genes in dirrheagenic E.coli isolates bore the prevalence of 86% (Bhakat et al, 2019) in India. The shiga like toxin was selected because these are one of the most potent toxin produced by E.coli that causes haemolytic diarrhea or Haemolytic Uremic Syndrome (HUS) in humans.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Escherichia Coli Isolates from Food Animals

Debbarma

Bora

Hazarika

et al. 2019

IJAR

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The present study was carried out for isolation and molecular characterization of Escherichia coli from faecal and meat samples of food animals viz. cattle, pigs and poultry. A total of 66 E. coli isolates were recovered from 420 samples of different food animals and further confirmed by PCR targeting E. coli specific uidA gene. These isolates were sensitive to chloramphenicol and ciprofloxacin and resistant to ampicillin and cloxacillin. Out of 66 isolates, 42 were typed into 13 different ‘O’ serogroups, 13 untypable and remaining 11 were identified as rough. Serogroup O84, O101, O118, O120 and O147 were predominant and serogroup O118 was found to be common in the samples of all 3 species of food animals. Five (7.57%) and 3 (4.54%) of E. coli isolates were found to harbor virulent genes, stx2 and est, respectively. Twenty representative E. coli isolates selected randomly from 20 different locations were subjected to molecular typing by PCR targeting Repetitive Extragenic Palindromic (REP) sequences. The region specific molecular types of E. coli could not be detected by using REP-PCR based discrimination.

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Identification of common virulence factors present in enterotoxigenicEscherichia coliisolated from diarrhoeal patients in Kolkata, India

Abstract: Prevalence of ETEC virulence factors would help in tracking ETEC globally and suggests the need of a multivalent ETEC vaccine.

Cited by 8 publications

References 36 publications

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

The Emergence of Extended-Spectrum β-Lactamase Producing and Hybrid Pathotypes of Escherichia Coli Isolates from Diarrheic Human Cases: A New Public Health Concern in Iran

Distribution of virulence factors and its relatedness towards the antimicrobial response of enterotoxigenic Escherichia coli strains isolated from patients in Kolkata, India

Molecular Characterization of Escherichia Coli Isolates from Food Animals

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