Identification of complex, naturally occurring flavonoid glycosides in Vicia faba and Pisum sativum leaves by HPLC-DAD-ESI-MSn and the genotypic effect on their flavonoid profile

Determination of lignans and phenolic components of Schisandra chinensis (Turcz.) Baill. using HPLC-ESI-ToF-MS and HPLC-online TEAC: Contribution of individual components to overall antioxidant activity and comparison with traditional antioxidant assays

“…The assignment of different sugar substitutions at the flavonoid aglycon was carried out in accordance with previous studies of Ablajan et al (2006), Neugart, Rohn, and Schreiner (2015), and Vallejo, Tomás-Barberán, and Ferreres (2004). These studies demonstrated that the first fragmentation of the pseudomolecular ion As a peculiarity, compounds 12′, and 15′ which were identified as quercetin-3-O-galactoside and quercetin-3-O-xyloside, presented a characteristic fragmentation behaviour.…”

Responses of flavonoid profile and associated gene expression to solar blue and UV radiation in two accessions of Vicia faba L. from contrasting UV environments

“…In our experiment, quercetin glycosides were the main flavonoids in Aurora and ILB938, whereas kaempferol glycosides were the main flavonoids in the leaves of German winter (Hiverna and Nordica) and spring (Fuego and Espresso) cultivars of V. faba grown under greenhouse conditions, with quercetin having a relatively minor role. 32 The differences may be due to the absence of UV treatment in the earlier experiment and/or genetic differences between the tested germplasm. The role of blue light in increasing the concentration of flavonoids in the epidermis Table 2 Flavonoid and phenolic acid compounds identified by HPLC-MS n in the leaves of accessions Aurora and ILB938 of V. faba, abbreviation for the compounds, significances of the main effects of accession and filter as well as of the interaction of accession and filter, and weightings in princi a The first number in the label stands for the number of sugars attached to the aglycone, and when "acetyl" is in the label, the compound is acetylated.…”

“…The flow rate was 0.85 ml min −1 and the detector wavelengths were 280 nm, 320 nm, 330 nm, 370 nm and 520 nm. The hydroxycinnamic acid derivatives and glycosides of flavonoids were identified as deprotonated molecular ions and characteristic mass fragment ions according to Schmidt et al 31 and Neugart et al 32 by HPLC-DAD-ESI-MS n using a Bruker Amazon SL ion trap mass spectrometer (Agilent Technologies, Waldbronn, Germany) in negative ionisation mode. Nitrogen was used as the dry gas (10 l min −1 , 325°C) and the nebulizer gas (40 psi) with a capillary voltage of −3500 V. Helium was used as the collision gas in the ion trap.…”

Intercropping Induces Changes in Specific Secondary Metabolite Concentration in Ethiopian Kale (Brassica carinata) and African Nightshade (Solanum scabrum) under Controlled Conditions

“…To analyze the phenolic compounds, 20 mg lyophilized powdered leaf sample was extracted according to Neugart et al (2015) . For the quantitative analysis of flavonoid glycosides and hydroxycinnamic acid derivatives, an HPLC series 1100 (Agilent Technologies, Waldbronn, Germany) was used as described in Neugart et al (2017) .…”