Identification of Contamination Sources and Assessment of Risk Factors Associated with the Occurrence of Escherichia coli O157:H7 on Small-scale Cow-calf Operations in Oklahoma and Louisiana

Jaroni, Divya A.; Saha, Joyjit; Rumbaugh, Kaylee; Marshall, Renita Woods

doi:10.1016/j.jfp.2023.100156

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…The STEC isolates used in the study included, E. coli O157:H7 (ATCC 43895, wild type (WT): LF4, KF10); O26 (CDC 2003-3014, WT: QF6, BF8); O45 (CDC 2000-3039, WT: EF2, AF1); O103 (CDC 2006-3008, WT: GF6, AF10); O111 (CDC 2010C-3114, ATCC: 2440, 2180), O121 (CDC 2002-3211, ATCC: 2219, 2203); and O145 (CDC 99-3311, ATCC: 2208, 1652). The WT isolates were retrieved from the Jaroni laboratory culture collection, originally isolated from bovine feces or cattle farm environment [30]. Biofilm forming capability of these isolates was previously tested in-vitro [31].…”

Section: Bacterial Culturesmentioning

confidence: 99%

Effectiveness of Bacteriophages Against Biofilm-forming Shiga-toxigenic <em>Escherichia coli In-vitro</em> and on Food-Contact Surfaces

Jaroni¹,

Litt²,

Bule³

et al. 2023

Preprint

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(1) Background: Formation of biofilms on food-contact surfaces by Shiga-toxigenic Escherichia coli (STEC) can pose a significant challenge to the food industry, making conventional control-methods insufficient. Targeted use of bacteriophages to disrupt these biofilms could reduce this problem. Previously isolated and characterized bacteriophages (n=52) were evaluated against STEC biofilms in-vitro and on food-contact surfaces. (2) Methods: Phage-treatments (9 logs PFU/ml), in phosphate-buffered-saline, were used individually or as cocktails. Biofilms of STEC (O157, O26, O45, O103, O111, O121, and O145) were formed in 96-well micro-titer plates (7 logs CFU/ml; 24 h) or on stainless-steel (SS) and high-density-polyethylene (HDPE) coupons (9 logs CFU/cm2; 7 h), followed by phage-treatment. Biofilm-disruption was measured in-vitro at 0, 3, and 6 h as a change in optical-density (A595). Coupons were treated with STEC-serotype-specific phage-cocktails or a 21-phage cocktail (3 phages/serotype) for 0, 3, 6 and 16 h and surviving STEC populations enumerated. (3) Results: Of the 52 pages, 77% showed STEC-biofilm disruption in-vitro. Serotype-specific phage-treatments reduced pathogen population within the biofilms by 1.9-4.1 and 2.3-5.6 logs CFU/cm2, while the 21-pahge cocktail reduced it by 4.0 and 4.8 logs CFU/cm2 on SS and HDPE, respectively. (4) Conclusions: Bacteriophages can be used to reduce STEC and their biofilms.

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Section: Bacterial Culturesmentioning

confidence: 99%

Effectiveness of Bacteriophages Against Biofilm-forming Shiga-toxigenic <em>Escherichia coli In-vitro</em> and on Food-Contact Surfaces

Jaroni¹,

Litt²,

Bule³

et al. 2023

Preprint

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Visual Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid On-Site Detection of Escherichia coli O157: H7 in Milk Products

Cui,

Wei,

et al. 2024

Foods

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(1) Background: Rapid on-site testing is an effective method for the detection of Escherichia coli O157: H7(E. coli O157: H7) in food ingredients and the environment. (2) Methods: In this study, we developed colorimetric loop-mediated isothermal amplification (LAMP) and immunochromatographic test strips (ICTs) for the rapid and visual detection of E. coli O157: H7. This study designed new specific LAMP primers for E. coli O157: H7 virulence island genes. After the LAMP amplification, the double-stranded DNA target sequence labeled with digoxin and fluorescein isothiocyanate (FITC) at both ends was bound to the anti-digoxin antibody on the gold nanoparticles. Subsequently, it was further bound to the anti-FITC antibody at the T line of the ICTs, forming a positive test result. Hydroxynaphthyl blue dye was directly added to the LAMP amplification product. A blue color indicated positive results, while a purple color indicated negative results. (3) Results: Two visualization methods showed high specificity for the target strains. The visualization tests had sensitivities of 5.7 CFU mL−1, and the detection limit of the Escherichia coli O157: H7 in artificially contaminated milk samples was 5.7 × 102 CFU mL−1, which was consistent with the results of the standard method (LAMP-electrophoresis method) used in commercial inspection. (4) Conclusions: Both methods could be useful in remote and under-resourced areas.

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Identification of Contamination Sources and Assessment of Risk Factors Associated with the Occurrence of Escherichia coli O157:H7 on Small-scale Cow-calf Operations in Oklahoma and Louisiana

Cited by 2 publications

References 41 publications

Effectiveness of Bacteriophages Against Biofilm-forming Shiga-toxigenic <em>Escherichia coli In-vitro</em> and on Food-Contact Surfaces

Effectiveness of Bacteriophages Against Biofilm-forming Shiga-toxigenic <em>Escherichia coli In-vitro</em> and on Food-Contact Surfaces

Visual Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid On-Site Detection of Escherichia coli O157: H7 in Milk Products

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