Identification of Corneal Neovascularization–Related Long Noncoding RNAs Through Microarray Analysis

Huang, Jin; Li, Yujie; Liu, Jingyu; Zhang, Yangyang; Li, Xiu‐Miao; Wang, Linnong; Yao, Jin; Jiang, Qin; Yan, Biao

doi:10.1097/ico.0000000000000389

Cited by 23 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A 2.5 mm diameter filter paper soaked with 0.1 M NaOH was placed on the central cornea for 30 s, followed by immediate rinsing with 0.9% saline solution for 10 s. Areas of corneal neovascularization were determined using Image J software package The neovascular area was determined by subtracting non-stimulated vascular area from vascular area [23]. …”

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA-MIAT promotes neurovascular remodeling in the eye and brain

et al. 2016

Self Cite

View full text Add to dashboard Cite

Although nervous and vascular systems are functionally different, they usually share similar mechanisms for function maintenance. Neurovascular dysfunction has became the pathogenesis of several vascular and nervous disorders. Here we show that long non-coding RNA-MIAT is aberrantly expressed under neurovascular dysfunction condition. MIAT is shown as a regulator of vascular dysfunction, including retinal angiogenesis, corneal angiogenesis, and vascular permeability. MIAT is also shown as a regulator of retinal neurodegeneration under diabetic condition. Mechanistically, MIAT regulates neural and vascular cell function via MIAT/miR-150-5p/VEGF network. The eye is a valuable model to study central nervous system (CNS) disorders. We show that MIAT knockdown leads to cerebral microvascular degeneration, progressive neuronal loss and neurodegeneration, and behavioral deficits in a CNS neurovascular disorder, Alzheimer's disease. MIAT may represent a pharmacological target for treating neurovascular-related disorders.

show abstract

Section: Methodsmentioning

confidence: 99%

Long non-coding RNA-MIAT promotes neurovascular remodeling in the eye and brain

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…LncRNAs have been reported to be involved in the pathogenesis of several ocular diseases, including corneal neovascularization [32], diabetic retinopathy (DR) [19,33], proliferative vitreoretinopathy (PVR) [34], age-related macular degeneration [35] and cataract [16]. In this study, we used lncRNA sequencing to identify lncRNAs involved in nuclear cataractgenesis.…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Liu

Shan

et al. 2018

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: Age-related cataract (ARC) remains the leading cause of visual impairment among the elderly population. Long non-coding RNAs (lncRNAs) have emerged as potential regulators in many ocular diseases. However, the role of lncRNAs in nuclear ARC, a subtype of ARC, requires further elucidation. Methods: LncRNA sequencing was performed to identify differentially expressed lncRNAs between the capsules of transparent and nuclear ARC lenses. Expression validation was confirmed by qRT-PCR. MTT assay, Calcein-AM and propidium iodide double staining, Rhodamine 123 and Hoechst double staining, EdU and transwell assay were used to determine the role of H19 or miR-675 in the viability, apoptosis, proliferation and migration of primary cultured human lens epithelial cells (HLECs). Bioinformatics and luciferase reporter assays were used to identify the binding target of miR-675. Results: Sixty-three lncRNAs are differentially expressed between the capsules of transparent and nuclear ARC lenses. One top abundantly expressed lncRNA, H19, is significantly up-regulated in the nuclear ARC lens capsules and positively associated with nuclear ARC grade. H19 knockdown accelerates apoptosis development and reduces the proliferation and migration of HLECs upon oxidative stress. H19 is the precursor of miR-675, and a reduction of H19 inhibits miR-675 expression. miR-675 regulates CRYAA expression by targeting the binding site within the 3’UTR. Moreover, miR-675 increases the proliferation and migration while decreasing the apoptosis of HLECs upon oxidative stress. Conclusion: H19 regulates HLECs function through miR-675-mediated CRYAA expression. This finding would provide a novel insight into the pathogenesis of nuclear ARC.

show abstract

“…established a murine CNV model and performed lncRNA expression profiling. They found that NR_033585 was highly expressed in CNV and exhibited an expression pattern similar to proangiogenic factors such as VEGF, matrix metalloproteinase‐9 and angiopoietin II, while the lincRNA:chr8:129102060‐129109035 reverse strand was significantly lower in CNV and exhibited an expression pattern similar to anti‐angiogenic factors, such as platelet‐derived growth factor . The study provides evidence that lncRNAs have pro‐angiogenic or anti‐angiogenic roles in vascularization.…”

Section: Roles Of Lncrnas In Ocular Diseasesmentioning

confidence: 88%

Long non‐coding RNAs in ocular diseases: new and potential therapeutic targets

et al. 2019

View full text Add to dashboard Cite

Long non‐coding RNAs (lncRNAs) are non‐protein coding transcripts containing more than 200 nucleotides. In the past, lncRNAs were considered as ‘transcript noise’ or ‘pseudogenes’ and were thus ignored. However, in recent years, lncRNAs have been proven to regulate gene expression at the epigenetic, transcriptional and translational level, and thereby influence cell proliferation, apoptosis, viability, immune response and oxidative stress. Furthermore, increasing evidence points to their involvement in different diseases, including cancer and heart diseases. Recently, lncRNAs were shown to be differentially expressed in ocular tissues and play a significant role in the pathogenesis of ophthalmological disorders such as glaucoma, corneal diseases, cataract, diabetic retinopathy, proliferative vitreoretinopathy and ocular tumors. In this review, we summarize the classification and mechanisms of known lncRNAs, while detailing their biological functions and roles in ocular diseases. Moreover, we provide a concise review of the clinical relevance of lncRNAs as novel, potential therapeutic targets in the treatment of eye diseases.

show abstract

Identification of Corneal Neovascularization–Related Long Noncoding RNAs Through Microarray Analysis

Abstract: This study provides a novel insight into CN pathogenesis. The intervention of dysregulated lncRNAs may become potential targets for the prevention and treatment of ocular vascular diseases.

Cited by 23 publications

References 29 publications

Long non-coding RNA-MIAT promotes neurovascular remodeling in the eye and brain

Long non-coding RNA-MIAT promotes neurovascular remodeling in the eye and brain

Long Non-Coding RNA H19 Regulates Human Lens Epithelial Cells Function

Long non‐coding RNAs in ocular diseases: new and potential therapeutic targets

Contact Info

Product

Resources

About