Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR

Çetinkaya, Burhan; Karahan, Murat; Atil, Eray; Kalın, Recep; Baère, Thierry De; Vaneechoutte, Mario

doi:10.1016/s0378-1135(02)00089-5

Cited by 88 publications

(77 citation statements)

References 21 publications

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“…There was no amplification in the controls (Figure 5). The present of molecular diagnosis by using PCR for pld gene is agreement with previously studies (Cetinkaya, et al, 2002;Pacheco et al, 2006;Ali et al, 2016). …”

Section: Resultssupporting

confidence: 91%

Suppurative Pneumonia and Lymphadenitis in a Goat Associated with Infection by Corynebacterium Pseudotuberculosis A Case Study

Singh¹,

Kumari²,

Yadav³

et al. 2017

Adv. Anim. Vet. Sci.

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Section: Resultssupporting

confidence: 91%

Suppurative Pneumonia and Lymphadenitis in a Goat Associated with Infection by Corynebacterium Pseudotuberculosis A Case Study

Singh¹,

Kumari²,

Yadav³

et al. 2017

Adv. Anim. Vet. Sci.

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“…The IFN-γ ELISA test appears to be more sensitive than the normal antibody ELISA in detecting prior infection in goats, and it does not seem to be affected by vaccination in sheep [71]. Another novel strategy that holds promise for the diagnosis of CLA is the use of polymerase chain reaction (PCR) tests specific for C. pseudotuberculosis to identify bacteria isolated from abscesses [21].…”

Section: Diagnosis and Control Of Clamentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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-Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis (CLA), a common disease in small ruminant populations throughout the world. Once established, this disease is difficult to eradicate because drug therapy is not effective and because the clinical detection of infected animals is of limited efficiency. We reviewed the microbiological, biochemical and taxonomic features of C. pseudotuberculosis, general aspects of infection, the main virulence determinants and currently available commercial vaccines. We also examined the current molecular strategies for the study of virulence in C. pseudotuberculosis, including the latest research on the identification of novel virulence factors and genes, which will help us to better understand the biology of this microorganism. This knowledge may also contribute to the development of improved CLA vaccines, including subunit and DNA-based types, as well as to improve the diagnosis, treatment and control of this disease.

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“…It is suggested there may be a close genotypic relationship between C. pseudotuberculosis isolates of small ruminant origin from different regions of the world, given the disease spread following new migrations and sheep exports to Australia and the United States (Baird and Fontaine 2007). However, disease spread is reported to be lower than disease prevalence in many countries, where it is stated at 8-90% (Erganis et al 1990;Cetinkaya et al 2002;Al-Gaabary et al 2009;Guimaraes et al 2009;Seyffert et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Corynebacterium pseudotuberculosis in sheep

et al. 2018

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Caseous lymphadenitis is still a serious zoonotic problem in Turkey. Sheep suffer from the disease with yield loss in wool and meat production. Moreover, with inexperienced laboratory staff, biochemical identification may go unrevealed. The scope of this study was to demonstrate the presence of Corynebacterium pseudotuberculosis in sheep by PCR. The sampling was conducted via collecting lymph fluids from the lymph node internal pouch wall of 100 sheep that were examined for the presence of Corynebacterium pseudotuberculosis. Molecular identification of the Corynebacterium pseudotuberculosis isolates was carried out by establishing the presence of the proline iminopeptidase gene. All isolates were confirmed to be Corynebacterium pseudotuberculosis by polymerase chain reaction. The polymerase chain reaction procedure conducted in this research was observed to be reliable and fast, and could be utilized for confirmation of caseous lymphadenitis in sheep as an optional technique to timeconsuming biochemical identification methods. Pleomorphic bacteria, caseous lymphadenitis, proline iminopeptidase, PCR

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Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR

Cited by 88 publications

References 21 publications

Suppurative Pneumonia and Lymphadenitis in a Goat Associated with Infection by Corynebacterium Pseudotuberculosis A Case Study

Suppurative Pneumonia and Lymphadenitis in a Goat Associated with Infection by Corynebacterium Pseudotuberculosis A Case Study

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Molecular identification of Corynebacterium pseudotuberculosis in sheep

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