2019
DOI: 10.1021/acs.biomac.9b00609
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Identification of Critical Surface Parameters Driving Lectin-Mediated Capture of Bacteria from Solution

Abstract: Lectin-functional interfaces are useful for isolation of bacteria from solution because they are low-cost and allow nondestructive, reversible capture. This study provides a systematic investigation of physical and chemical surface parameters that influence bacteria capture over lectin-functionalized polymer interfaces and then applies these findings to construct surfaces with significantly enhanced bacteria capture. The designer block copolymer poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) w… Show more

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Cited by 13 publications
(14 citation statements)
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“…300 μL of the mixture was added over each hydrogel and incubated in the dark for 15 min. SYTO 9 labels both intact and compromised cells, while propidium iodide labels only cells with damaged membranes, resulting in the reduction of expressed fluorescence by SYTO 9 . After staining, the hydrogels were washed thoroughly with a 0.85 wt % NaCl solution and imaged using the inverted fluorescence microscope.…”
Section: Methodsmentioning
confidence: 99%
“…300 μL of the mixture was added over each hydrogel and incubated in the dark for 15 min. SYTO 9 labels both intact and compromised cells, while propidium iodide labels only cells with damaged membranes, resulting in the reduction of expressed fluorescence by SYTO 9 . After staining, the hydrogels were washed thoroughly with a 0.85 wt % NaCl solution and imaged using the inverted fluorescence microscope.…”
Section: Methodsmentioning
confidence: 99%
“…Furthermore, this study is the first showing the effects of antifungal coating towards C. auris. There are a number of studies demonstrating that high-aspect-ratio nanostructures can be used to capture bacteria and disrupt bacterial membranes [18,[37][38][39][40]. Surface nanostructures have been considered to be "promising methods" to stop bacterial adhesion and proliferation; still, there are a number of questions that should be answered before being applied in the actual environments [41].…”
Section: Microbicidal Performance Of Ag Nps-cu Surfaces Over 1-and 7-day Incubationsmentioning
confidence: 99%
“…They are capable of transforming biological response into an electric signal. They include two major components: biorecognition elements (antibody, nucleic acids, enzymes, whole cells, peptides, lectins or glycans), which recognize the target, and physico-chemical transducers, for converting the recognition event into an assessable signal proportional to the analyte target concentration [85,86,87].…”
Section: Label-based and Label-free Biosensorsmentioning
confidence: 99%